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Method for cultivating liquid-state morchella strain

A liquid strain and culture method technology, applied in the biological field, can solve the problems of morel strain growth quality, cumbersome cultivation steps, high labor intensity, etc., and achieve the benefits of absorption and utilization, good cultivation effect, and low utilization rate Effect

Inactive Publication Date: 2015-12-16
宋瑞鹏
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although there are reports at present that the artificial cultivation of hickory chicks has been realized, the components of the hickory chick strain culture medium used often have a greater impact on the growth quality of the hickory chick strains. Need to go through multiple steps such as inoculation, static culture and shake flask culture, and also need to manually introduce sterile air into the culture vessel, resulting in cumbersome culture steps and high labor intensity

Method used

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  • Method for cultivating liquid-state morchella strain

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Embodiment 1

[0028] The present embodiment provides a kind of cultivation method of hickory chick liquid bacterial classification, selects the original species of hickory chick No. 926 as hickory chick cultivar to cultivate, specifically comprises the following steps:

[0029] Mix 200g of pine branches, 200g of potatoes and 1800ml of water, boil and keep for 30 minutes, then cool to room temperature, and filter to obtain a filtrate; add 30g of sucrose to the filtrate and dissolve to obtain a morel liquid strain nutrient filtrate 150ml of the nutrient filtrate of the hickory chick liquid strain is taken and placed in a culture bottle, then 20 g of cleaned and dried chaff and 5 g of calcium carbonate are added to the culture bottle to prepare the hickory chick liquid strain culture medium .

[0030] Place the morel liquid strain culture medium in a sterilizing pot, sterilize it at 1.5kg / cm3 and 110°C for 0.5 hours, take it out and cool it to room temperature, and culture the morel liquid str...

Embodiment 2

[0040]The present embodiment provides a kind of cultivation method of hickory chick liquid bacterial classification, selects morel 926 original species of hickory chick to cultivate as the bacterial strain of hickory chick cultivar, and concrete cultivation step is as follows: pine branch 300g, potato 400g and Mix 1800ml of water, boil and keep for 30 minutes, then cool to room temperature, and filter to obtain the filtrate; take 400g of fresh wheat grains, soak them in clean water for 12 hours, let them germinate at room temperature, and wait until the sprouts are about the size of wheat grains 3 times, take it out and dry it at 60-80°C, grind it into malt powder, then add the malt powder into the filtrate, the added filtrate is 6 times of the malt powder, at 50°C After 6 hours of saccharification and filtering through 4 layers of gauze, the morel liquid strain nutrient filtrate was obtained; 150 ml of the morel liquid strain nutrient filtrate was taken and placed in a culture...

Embodiment 3

[0044] The present embodiment provides a method for cultivating liquid strains of morels. The original species of hickory chick no. The medium raw materials used in the cultivation process of No. 926 cultivar are as follows: wheat bran 40g, corn flour 20g, soybean flour 10g, KH2PO41g, MgSO41g. The average biomass of Duji 926 was 7.148g / L, and the production cost was 0.465 yuan / g.

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Abstract

The invention provides a method for cultivating a liquid-state morchella strain. The method includes the steps that a liquid-state morchella strain culture medium is provided; a stock morchella strain is inoculated into the liquid-state morchella strain culture medium for conducting airtight culture; after hyphae of the stock morchella strain germinate, the liquid-state morchella strain culture medium is shaken once every day till a morchella cultivated species is obtained. According to the method for cultivating the liquid-state morchella strain, operation is easy, the cultivated hyphae of the morchella cultivated species grow fast, the produced hyphae are even and neat, contamination by other strains is small, and the artificial cultivation survival rate and yield of morchella are increased; moreover, the culture process is simple and easy to implement, and cost is low.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for cultivating liquid strains of hickory chick. Background technique [0002] Morchella is a precious and rare wild edible fungus with high nutritional value. Although there are reports at present that the artificial cultivation of hickory chick has been realized, the components of the hickory chick culture medium used often have a greater impact on the growth quality of the hickory chick. Multiple steps such as inoculation, static culture and shake flask culture are required, and sterile air needs to be artificially introduced into the culture vessel, resulting in cumbersome culture steps and high labor intensity. Therefore, it is of great practical significance to the cultivation of hickory chick to design a kind of cultivation method of reasonable hickory chick liquid strain substratum and a kind of cultivation technology simple hickory chick liquid strain culture mediu...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12R1/645
Inventor 宋瑞鹏
Owner 宋瑞鹏
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