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Preparation method of high-purity rubusoside

A kind of sweet tea glycoside, high-purity technology, applied in the field of high-purity sweet tea glycoside preparation, can solve the problems of high equipment requirements, environmental pollution, solvent residue and the like

Inactive Publication Date: 2015-12-23
CHONGQING JOYWIN NATURAL PROD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the extraction method of high-purity rubusoside generally includes: extraction→concentration→centrifugation→column separation→concentration (recovery of ethanol)→recrystallization→drying. Separation uses one or more of macroporous adsorption resin columns, amide columns, ion exchange resin columns or chromatographic columns, all of which are accompanied by the use and recovery of a large amount of organic solvents, which not only pollute the environment but also result in products separated by columns It needs to be processed by eluent, the concentration of rubusoside solution after elution is low, and it needs to be concentrated before recrystallization can be carried out. However, the existing concentration is generally carried out by controlling the pressure to evaporate, which requires high equipment. Solvent residues may exist during the concentration process of recovered ethanol

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] 100g sweet tea leaves are crushed through a 40-mesh sieve, added with 1.5 liters of water, extracted twice at 85°C, the extract is filtered to remove insoluble matter, the filtrate is passed through an ultrafiltration membrane system with a molecular weight cut-off of 5KDa, and the ultrafiltration permeate is passed through a reverse osmosis membrane system Make the solid content in the concentrated solution reach 15%, and the concentrated solution is dried in a hot air circulation oven or granulated by a spray tower to obtain 5.4g of a yellow product with a purity of 74.42%. The 5.4g product is dissolved in 43ml of absolute ethanol at 60°C. Hot filtration, the filtrate was left standing at room temperature for 24 hours, filtered to obtain white crystals, and dried in a hot air circulation oven to obtain 2.9g of a white product with a purity of 91.58%. Dissolve 2.9g of the product in 20ml of absolute ethanol at 60°C. After hot filtration and cooling, the filtrate was lef...

Embodiment 2

[0021] 100g of sweet tea leaves are crushed and passed through a 40-mesh sieve, added with 1.5 liters of water, extracted twice at 90°C, the extract is filtered to remove insoluble matter, the filtrate passes through an ultrafiltration membrane system with a molecular weight cut-off of 10KDa, and the ultrafiltration permeate passes through a reverse osmosis membrane system Make the solid content in the concentrated solution reach 15%. The concentrated solution is dried in a hot air circulation oven or granulated by a spray tower to obtain 6.2g of a brownish-yellow product with a purity of 68.54%. The 6.2g product is dissolved in 62ml of absolute ethanol at 60°C. Filtrate while hot, let the filtrate stand at room temperature for 24 hours, filter to obtain white crystals, and dry in a hot air circulation oven to obtain 3.1g of a white product with a purity of 89.26%. Dissolve 3.1g of the product in 25ml of absolute ethanol at 60°C. After cooling while hot, the filtrate was allowe...

Embodiment 3

[0023] 100g of sweet tea leaves is crushed through a 20-mesh sieve, added with 1.5 liters of water, extracted twice at 65°C, the extract is filtered to remove insoluble matter, the filtrate is passed through an ultrafiltration membrane system with a molecular weight cut-off of 1KDa, and the ultrafiltration permeate is passed through a reverse osmosis membrane system Make the solid content in the concentrated solution reach 10%, and the concentrated solution is dried in a hot air circulation oven or granulated by a spray tower to obtain 5.5 g of a brownish-yellow product with a purity of 67.22%, and 5.5 g of the product is dissolved in 50 ml of absolute ethanol at 60° C. Filtrate while hot, let the filtrate stand at room temperature for 24 hours, filter to obtain white crystals, and dry in a hot air circulation oven to obtain 2.7g of a white product with a purity of 89.71%. Dissolve 2.7g of the product in 19ml of absolute ethanol at 60°C. After cooling while hot, the filtrate wa...

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Abstract

The invention discloses a preparation method for high-purity rubusoside and relates to the field of food processing. In order to overcome the defects, the preparation method for high-purity rubusoside is simple in treatment process, free of contamination and low in energy consumption and makes the product high in purity. The preparation method includes the following steps that 1, strigose hydrangea leaves are smashed, and through water extraction, strigose hydrangea powder extract is obtained; 2, the extract passes through a hollow fiber ultrafiltration membrane and a reverse osmosis membrane to obtain concentrated liquor; 3, the concentrated liquor is dried and smashed to obtain a rubusoside crude product; 4, the rubusoside crude product is dissolved through ethyl alcohol, and then filtering and recrystallizing are performed to obtain a primary-crystallization product; 5, the primary-crystallization product is dissolved through ethyl alcohol, and filtering and recrystallizing are performed to obtain high-purity rubusoside. The preparation method is free of contamination in the treatment process, through a film system, separating and concentrating are combined, treatment difficulty is lowered, and the purity of obtained rubusoside can reach over 98%.

Description

technical field [0001] The invention relates to the field of food processing, in particular to a method for preparing high-purity rubusoside. Background technique [0002] sweet tea ( Rubussuavissimus S. Lee ) is a perennial deciduous shrub of the genus Rubus in the Rosaceae family. It is also known as the three major sweet plants in Guangxi together with Luo Han Guo and Stevia. Sweet tea contains rubusoside, a sweet substance with excellent flavor. [0003] Rubusoside, also known as sweetener and rubusoside, is a tetracyclic diterpene glycoside formed by combining Steviol and glucose, with molecular formula C 32 h 50 o 13 , molecular weight 642.739. The sweetness of rubusoside is about 300 times that of sucrose, and its calorific value is only 1% of that of sucrose. It is a natural sweetener with high sweetness and low calorie value, suitable for diabetes, hypertension, obesity, hyperlipidemia It is widely used in the market of natural non-sugar sweeteners such as foo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23F3/18
Inventor 杨刚柱徐小琴卫伟薛荣涛
Owner CHONGQING JOYWIN NATURAL PROD
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