Method for extracting and gathering total alkaloids of hypecoum leptocarpum

A technology of fennel and total alkaloids, which is applied in the direction of pharmaceutical formula, plant raw materials, medical preparations containing active ingredients, etc., can solve the problems of high production cost, unsuitable for industrial production, flammability and explosion, and achieve Cost savings, avoidance of damage, good mechanical strength and reproducible effects

Active Publication Date: 2015-12-23
CHINA ACAD OF SCI NORTHWEST HIGHLAND BIOLOGY INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Among them, the extraction method of alkaloids mostly uses 90%~95% ethanol as the extraction solvent, and steps such as pH value adjustment are added; the published Chinese patent CN104327071A "Method for extracting and separating four kinds of alkaloids from the Tibetan medicine fennel" adopts Total alkaloids are extracted with ethanol with a volume fraction of 70-95%. The extraction solvent used in this type of extraction method is high-concentration ethanol. In actual production, there are not only the disadvantages of high production costs, but also the safety risk of being flammable and explosive. Therefore, this type of process is not suitable for use in industrial production.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1 A method for extracting and enriching total alkaloids of anise fennel, comprising the following steps:

[0023] (1) Extraction: Grind 1.58Kg of the fennel medicinal material to 20 meshes, and extract it with ethanol with a volume concentration of 10% of 15 times its mass to obtain an extract, which is kept under the condition of a vacuum of -0.09MPa Recover the ethanol to the liquid extract under vacuum and decompression to obtain the fennel extract.

[0024] Wherein: the extraction condition refers to that the temperature is room temperature, and the number of extractions is 3 times, 24 hours each time.

[0025] (2) Enrichment of alkaloids: After dissolving the extract of fennel fennel with 10 times its mass of pure methanol, it is first treated with silica SCX column of strong cation exchange material with silica gel matrix, and then sequentially treated with 8 times of column volume of pure methanol and 3 times of column volume The ammonium perchlorate-m...

Embodiment 2

[0029] Example 2 A method for extracting and enriching total alkaloids of anise fennel, comprising the following steps:

[0030] (1) Extraction: grind 20Kg of the fennel medicinal material to 20 meshes, and extract it with ethanol with a volume concentration of 69% of 6 times its mass to obtain an extract, which is vacuum-treated under the condition of a vacuum of 0 MPa. Recover the ethanol under reduced pressure to the liquid extract to obtain the fennel extract.

[0031] Wherein: the extraction condition refers to room temperature, and the number of extractions is 2 times, 48 ​​hours each time.

[0032] (2) Enrichment of alkaloids: After the fennel extract is dissolved with 2 times of its pure methanol, it is first treated with silica SCX column of strong cation exchange material with silica gel matrix, and then with 5 times of column volume of pure methanol and ammonium salt concentration of 5mmol / L ammonium perchlorate-methanol solution was used for elution, and the sal...

Embodiment 3

[0036] Example 3 A method for extracting and enriching total alkaloids of anise fennel, comprising the following steps:

[0037] (1) Extraction: Grind 0.5Kg of the fennel medicinal material into 60 meshes, and extract it with ethanol with a volume concentration of 55% 10 times its mass to obtain an extract, which is kept under the condition of a vacuum of -0.02MPa Recover the ethanol to the liquid extract under vacuum and decompression to obtain the fennel extract.

[0038] Wherein: the extraction condition refers to room temperature, and the number of extractions is 3 times, 36 hours each time.

[0039] (2) Enrichment of alkaloids: After dissolving the extract of fennel fennel with 5 times its mass of pure methanol, it is first treated with silica SCX column of strong cation exchange material with silica gel matrix, and then sequentially treated with 3 times of column volume of pure methanol and 5 times of ammonium The ammonium perchlorate-methanol solution with a salt con...

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PUM

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Abstract

The invention relates to a method for extracting and gathering total alkaloids of hypecoum leptocarpum. The method comprises the following steps: (1) extraction, crushing a medicinal material hypecoum leptocarpum, then leaching the hypecoum leptocarpum with ethanol with the volume concentration of 10%-69% to obtain an extract solution, and recovering ethanol from the extract solution through vacuum reduced pressure until a liquid extract is formed, so as to obtain a hypecoum leptocarpum extract; (2) gathering of alkaloids, dissolving the hypecoum leptocarpum extract, carrying out treatment with a strong cation exchange material silica SCX column of silica gel matrix, sequentially eluting the hypecoum leptocarpum extract with pure methanol and an ammonium perchlorate-methanol solution, and collecting a salt elution part; and (3) desalting treatment, carrying out desalting on the salt elution part with a polystyrene-based reverse resin column, eluting with water until colorless, eluting with pure methanol, collecting a pure methanol eluent, and recovering methanol from the pure methanol eluent, so as to obtain the total alkaloids of the hypecoum leptocarpum. The method is simple in technology, low in cost and high in efficiency.

Description

technical field [0001] The invention relates to a method for extracting total alkaloids, in particular to a method for extracting and enriching total alkaloids of anise fennel. Background technique [0002] The Tibetan medicine fennel fennel is Papaveraceae fennel genus fennel fennel ( Hypecoumleptocarpum The dry whole grass of Hook.f.etThoms.) grows on grasslands, meadows, and gravel ground below an altitude of 4,300 meters. "Jingzhu Materia Medica" records that it tastes bitter and cool, clears blood heat, warm and toxic heat; "Chinese Herbal Medicine Commonly Used in Tibet" claims that it has antipyretic, analgesic, anti-inflammatory and detoxifying effects, and can cure colds, headaches, limbs and joint pains , cholecystitis, and detoxification of food poisoning; "Shaangan Ningqing Chinese Herbal Medicine Selection" said: fennel with fine fruit horns can cure influenza, sore throat, red eyes and other diseases. [0003] Existing literature mainly studies the chemical c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/66
Inventor 文怀秀邵赟梅丽娟陶燕铎党军
Owner CHINA ACAD OF SCI NORTHWEST HIGHLAND BIOLOGY INST
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