Method for extracting and gathering total alkaloids of hypecoum leptocarpum

Abstract

The invention relates to a method for extracting and gathering total alkaloids of hypecoum leptocarpum. The method comprises the following steps: (1) extraction, crushing a medicinal material hypecoum leptocarpum, then leaching the hypecoum leptocarpum with ethanol with the volume concentration of 10%-69% to obtain an extract solution, and recovering ethanol from the extract solution through vacuum reduced pressure until a liquid extract is formed, so as to obtain a hypecoum leptocarpum extract; (2) gathering of alkaloids, dissolving the hypecoum leptocarpum extract, carrying out treatment with a strong cation exchange material silica SCX column of silica gel matrix, sequentially eluting the hypecoum leptocarpum extract with pure methanol and an ammonium perchlorate-methanol solution, and collecting a salt elution part; and (3) desalting treatment, carrying out desalting on the salt elution part with a polystyrene-based reverse resin column, eluting with water until colorless, eluting with pure methanol, collecting a pure methanol eluent, and recovering methanol from the pure methanol eluent, so as to obtain the total alkaloids of the hypecoum leptocarpum. The method is simple in technology, low in cost and high in efficiency.

Description

technical field [0001] The invention relates to a method for extracting total alkaloids, in particular to a method for extracting and enriching total alkaloids of anise fennel. Background technique [0002] The Tibetan medicine fennel fennel is Papaveraceae fennel genus fennel fennel ( Hypecoumleptocarpum The dry whole grass of Hook.f.etThoms.) grows on grasslands, meadows, and gravel ground below an altitude of 4,300 meters. "Jingzhu Materia Medica" records that it tastes bitter and cool, clears blood heat, warm and toxic heat; "Chinese Herbal Medicine Commonly Used in Tibet" claims that it has antipyretic, analgesic, anti-inflammatory and detoxifying effects, and can cure colds, headaches, limbs and joint pains , cholecystitis, and detoxification of food poisoning; "Shaangan Ningqing Chinese Herbal Medicine Selection" said: fennel with fine fruit horns can cure influenza, sore throat, red eyes and other diseases. [0003] Existing literature mainly studies the chemical c...

AI Technical Summary

Problems solved by technology

[0006] Among them, the extraction method of alkaloids mostly uses 90%~95% ethanol as the extraction solvent, and steps such as pH value adjustment are added; the published Chinese patent CN104327071A "Method for extracting and separating four kinds of alkaloids from the Tibetan medicine fennel" adopts Total alkaloids are extracted with ethanol with a volume fraction of 70-95%. The extraction solvent used in this type of extraction method is high-concentration ethanol. In actual production, there are not only the disadvantages of high production costs, but also the safety risk of being flammable and explosive. Therefore, this type of process is not suitable for use in industrial production.