Antimicrobial peptide XYZ-1 and application thereof

A technology of XYZ-1 and antibacterial peptide, which is applied to antibacterial peptide XYZ-1 and its application field to achieve the effect of broad antibacterial spectrum, high efficiency antibacterial effect and good clinical application effect

Inactive Publication Date: 2015-12-23
HENAN INST OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no unified understanding of the mechanism of action of antimicrobial peptides; howeve

Method used

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  • Antimicrobial peptide XYZ-1 and application thereof
  • Antimicrobial peptide XYZ-1 and application thereof
  • Antimicrobial peptide XYZ-1 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030]An antibacterial peptide XYZ-1, the amino acid sequence of the antibacterial peptide XYZ-1 is arginine-glycine-glycine-arginine-leucine-cysteine-tyrosine-cysteine-arginine Acid-arginine-arginine-proline-cysteine-valine-cysteine-valine-glycine-arginine-lysine (SEQ ID NO. 1).

[0031] The antimicrobial peptide XYZ-1 is a linear polypeptide containing 19 amino acid residues, a molecular weight of 2288.81 Da, and an isoelectric point of 10.83.

[0032] The antimicrobial peptide XYZ-1 product of this example is synthesized by an automatic peptide synthesizer according to a conventional solid-phase method, and the finally obtained antimicrobial peptide XYZ-1 is purified and analyzed by a semi-preparative high performance liquid chromatograph, and its purity is 95%.

[0033] Identification of the gained antimicrobial peptide XYZ-1 product of the present invention:

[0034] (1) Determination of molecular weight: use liquid chromatography mass spectrometry (WatersmicromassZQ-200...

Embodiment 2

[0039] Embodiment 2: the application implementation of antimicrobial peptide XYZ-1 of the present invention

[0040] 1. Detection of the antibacterial activity of the antibacterial peptide XYZ-1 of the present invention:

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Abstract

The invention discloses an antimicrobial peptide XYZ-1. The antimicrobial peptide XYZ-1 is a straight-chain peptide, and contains 19 amino acid residues, the molecular weight is 2288.81Da, the isoelectric point is 10.83, and the amino acid sequence of the antimicrobial peptide XYZ-1 is arginine-glycine-glycine-arginine-leucine-cysteine-tyrosine-cysteine-arginine-arginine-arginine-proline-cysteine-valine-cysteine-valine-glycine-arginine-lysine (SEQ ID NO.1). The antimicrobial peptide XYZ-1 disclosed by the invention does not produce hemolysis, the antimicrobial spectrum is wide, the antimicrobial peptide XYZ-1 has a highly effective antimicrobial effect on gram-positive bacteria, gram-negative bacteria and fungi, and therefore can be applied perfectly in the preparation of drugs for treating gram-positive bacterium, gram-negative bacterium or/and fungus disease infections, the clinical application effect of the antimicrobial peptide XYZ-1 is better, and moreover, the antimicrobial peptide XYZ-1 can also be used in the preparation of feed additives or food additives.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and in particular relates to an antibacterial peptide XYZ-1 and its application. Background technique [0002] Antimicrobial peptides (AMPs) are a class of defensive small peptides produced by biological organisms when resisting pathogenic microorganisms, and are an important part of the biological immune defense system. In addition to broad-spectrum antibacterial (Gram-negative bacteria and Gram-positive bacteria) activity, antimicrobial peptides also have a strong ability to kill fungi, viruses and parasites. Compared with traditional antibiotics, antimicrobial peptides have the advantages of small molecular weight, good thermal stability, good water solubility, broad antibacterial spectrum, are not easy to induce bacterial resistance, and do not produce drug allergic reactions. They are currently the most internationally recognized antibiotics. good substitute. [0003] However, natur...

Claims

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Application Information

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IPC IPC(8): C07K7/08A61K38/10A61P31/04A61P31/10A23K1/16A23L1/30
Inventor 王青徐彦召胡建和杭柏林孙亚伟刘保国王秋霞余娟张庆华
Owner HENAN INST OF SCI & TECH
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