Detecting kit for five kinds of food-borne pathogenic bacteria and detection method
A food-borne pathogenic bacteria and detection kit technology, applied in the fields of molecular biology and food safety detection, can solve the problems of unfavorable food quality control, poor detection sensitivity, long operation time, etc., and achieve fast detection speed and low consumption The effect of short time and simple detection procedure
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Embodiment 1
[0048] The test kit includes the following components:
[0049] (1) The concentration of the primer pair for Salmonella is 0.3 μmol / L: upstream primer 5'-GTCCAGCTCTGTCGCCTTAATC-3', downstream primer 5'-ACGGCTCCCTGCTACGCT-3';
[0050] (2) The primer pair concentration of Staphylococcus aureus is 0.3 μmol / L: upstream primer 5'-TCTGATTCAATTCGTGTTTACTATGA-3', downstream primer 5'-CGCTACCTTTAGGCACTGACA-3';
[0051] (3) The primer pair concentration of Escherichia coli O157:H7 is 0.5 μmol / L: upstream primer 5'-TATTTGATGATGATCCAGGGG-3', downstream primer 5'-ATTTTGTTCTCCGTCTTGTCCTA-3';
[0052] (4) The primer pair concentration of Vibrio parahaemolyticus is 0.5 μmol / L: upstream primer 5'-ACTAAGTTGGTCGGTTCGATATG-3', downstream primer 5'-TCTGGCTGCTTAGTTTGTGTTTAG-3';
[0053] (5) The concentration of the primer pair for Listeria monocytogenes is 0.9 μmol / L: upstream primer 5'-GTTAATGAACCTACAAGACCTTCC-3', downstream primer 5'-AACCGTTTCTCCACCATTCCCA-3';
[0054] (6) The concentration of ...
Embodiment 2
[0063] The test kit includes the following components:
[0064] (1) The concentration of the primer pair for Salmonella is 0.5 μmol / L: upstream primer 5'-GTCCAGCTCTGTCGCCTTAATC-3', downstream primer 5'-ACGGCTCCCTGCTACGCT-3';
[0065] (2) The primer pair concentration of Staphylococcus aureus is 0.5 μmol / L: upstream primer 5'-TCTGATTCAATTCGTGTTTACTATGA-3', downstream primer 5'-CGCTACCTTTAGGCACTGACA-3';
[0066] (3) The primer pair concentration of Escherichia coli O157:H7 is 0.3 μmol / L: upstream primer 5'-TATTTGATGATGATCCAGGGG-3', downstream primer 5'-ATTTTGTTCTCCGTCTTGTCCTA-3';
[0067] (4) The primer pair concentration of Vibrio parahaemolyticus is 0.3 μmol / L: upstream primer 5'-ACTAAGTTGGTCGGTTCGATATG-3', downstream primer 5'-TCTGGCTGCTTAGTTTGTGTTTAG-3';
[0068] (5) The concentration of the primer pair for Listeria monocytogenes is 0.7 μmol / L: upstream primer 5'-GTTAATGAACCTACAAGACCTTCC-3', downstream primer 5'-AACCGTTTCTCCACCATTCCCA-3';
[0069] (6) TaqDNA polymerase con...
Embodiment 3
[0078] The test kit includes the following components:
[0079] (1) The concentration of the primer pair for Salmonella is 0.4 μmol / L: upstream primer 5'-GTCCAGCTCTGTCGCCTTAATC-3', downstream primer 5'-ACGGCTCCCTGCTACGCT-3';
[0080] (2) The primer pair concentration of Staphylococcus aureus is 0.4 μmol / L: upstream primer 5'-TCTGATTCAATTCGTGTTTACTATGA-3', downstream primer 5'-CGCTACCTTTAGGCACTGACA-3';
[0081] (3) The primer pair concentration of Escherichia coli O157:H7 is 0.4 μmol / L: upstream primer 5'-TATTTGATGATGATCCAGGGG-3', downstream primer 5'-ATTTTGTTCTCCGTCTTGTCCTA-3';
[0082] (4) The primer pair concentration of Vibrio parahaemolyticus is 0.4 μmol / L: upstream primer 5'-ACTAAGTTGGTCGGTTCGATATG-3', downstream primer 5'-TCTGGCTGCTTAGTTTGTGTTTAG-3';
[0083] (5) The concentration of the primer pair for Listeria monocytogenes is 0.8 μmol / L: upstream primer 5'-GTTAATGAACCTACAAGACCTTCC-3', downstream primer 5'-AACCGTTTCTCCACCATTCCCA-3';
[0084] (6) The concentration of ...
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