A kind of aspergillus that transforms ginsenoside rb1 to produce rd and its application
A technology of ginsenoside and Aspergillus, which is applied in the transformation of Aspergillus ginsenoside Rb1 to produce Rd and the application field, and achieves the effect of high transformation activity
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0040] Screening of ginsenoside Rb1 transformed strain LFJ1403
[0041] (1) Take 1g of soil from the ginseng garden of Jilin Agricultural University, dissolve it in 5mL sterile water to obtain the mother solution of the soil, and then dilute the mother solution with sterile water for 10 -1 、10 -2 、10 -3 、10 -4 、10 -5 Five gradients. Take 200 μL of each gradient and coat a PDA plate containing 0.1 g / L chloramphenicol. Cultivate upside down at 30°C until single colonies grow out, and each grown single colony is continuously transferred to a PDA plate until a purified single colony is obtained and the slant is stored at 4°C for later use.
[0042] The 45 single bacterial colonies grown on the PDA plate were washed with an appropriate amount of 20mmol / L acetate buffer (pH=5.0), and at the same time, the bacterial spores were gently scraped off, transferred to a sterile Erlenmeyer flask, and shaken at 120rpm for 2 hours until The spores were mixed in the buffer. Filter the s...
Embodiment 2
[0047] Morphological and genetic identification of strain LFJ1403
[0048] Morphological identification of strain LFJ1403:
[0049] The spore suspension was obtained from the strain LFJ1403 according to the operation in step (1) of Example 1, and the PDA plate was coated with the spore suspension, cultured upside down at 30°C, and the colony morphology was observed every 24 hours and recorded by taking pictures. After observation, the colonies cultivated for 24 hours were white and fluffy, and some of the colonies were light green. After 48 hours of cultivation, the colony gradually becomes green and fluffy, the diameter of a single colony is 2-5 mm, and the back of the colony is light yellow. The colonies cultivated for 72 hours were completely gray-green and densely fluffy, the edges of the colonies were white, the back was dark yellow, and had a musty smell. The gray-green color of the colonies cultured for 96 hours continued to deepen, the spore clusters on the surface w...
Embodiment 3
[0063] 1. Determination of the best sporulation time
[0064] The aspergillus LFJ1403 spores cultured upside down on the PDA plate under the condition of 30° C. were washed down with the Tween80 solution of 0.1% by volume percentage concentration when culturing to 2, 3, 4, 5 and 6 days respectively, and the solvent of the Tween80 solution was pH= 5.0 acetate buffer to control the spore concentration at 5.0×10 6 cells / mL to make a spore suspension. The spore suspension prepared at the above 5 different sporulation times was mixed with an equal volume of 0.25mM ginsenoside Rb1 solution (the solvent of the ginsenoside Rb1 solution was acetate buffer at pH=5.0) and cultured at 30°C and 200rpm. The conversion rate of Rb1 was measured by HPLC analysis at 96 hours of transformation. The Rd yield of the spore suspension prepared by taking spores at 2 days and 3 days was higher than that at 4, 5, and 6 days, so the spores were taken at 2 days to 3 days Prepare the spore suspension tr...
PUM
| Property | Measurement | Unit |
|---|---|---|
| molecular weight | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
Login to View More