A method for extracting small molecule humic acid in soil by XAD resin

A humic acid and small molecule technology, applied in the preparation of test samples, etc., can solve the problems of lack and lack of knowledge of small molecule humic acid, and achieve the effect of improving the extraction efficiency

Inactive Publication Date: 2016-09-07
CHINESE RES ACAD OF ENVIRONMENTAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the severe lack of samples, people lack knowledge of small molecule humic acid

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 2

[0083] Embodiment 2: The difference between this embodiment and Embodiment 1 is that the small molecule humic acid solution obtained by each dialysis in step e described in this embodiment needs to measure the dissolved total organic carbon content therein, if If the dissolved total organic carbon content is >1.0mg / L, replace the ultrapure water in the dialysis system again, add ultrapure water each time, and after stirring for 20-40 hours, measure the total dissolved organic carbon in the small molecule humic acid solution again. carbon until the dissolved total organic carbon content in the small molecule humic acid solution is <0.5 mg / L; then proceed to step f.

Embodiment 3

[0084] Example 3: The difference between this example and Example 1 is that this example also includes step h: after drying at 80-100°C for 20-40 h, use a thermogravimetric analysis instrument to measure the soil obtained in step g Ash content of small molecule humic acid components;

[0085] If the ash content is greater than 1.0%, based on dry weight, under the condition of nitrogen protection, use NaOH to dissolve the soil small molecule humic acid component, add deionized water to make the concentration of humic acid in the solution = 1-3 g / L, Repeat steps f and g in turn until the ash content of the small-molecule humic acid component in the soil is less than 1.0%, on a dry weight basis.

Embodiment 4

[0086] Embodiment 4: Disclosed in this embodiment is a kind of method that XAD resin extracts small molecule humic acid in soil, comprises the following steps:

[0087] Step a: soil treatment: removing impurities such as tree roots and stones in the natural soil, after air-drying, grinding and sieving to obtain soil samples for subsequent use;

[0088] Step b: Sodium pyrophosphate extracts soil humic acid: add deionized water to the soil sample in step a to make the solid-liquid ratio reach 1:5-1:15, adjust the solution pH=1.0-3.0 with HCl and NaOH, and then Add HCl solution to it to a solid-to-liquid ratio of 1:10, stir continuously for 4-8 hours, then let stand for 20-40 hours, and centrifuge to obtain solid soil sample 1;

[0089] Add deionized water to solid soil sample 1 to make the solid-liquid ratio reach 1:3-1:5, adjust the pH of the solution to 6-8 with HCl and NaOH, and obtain a solid-liquid mixed solution; Add sodium pyrophosphate solution to the liquid mixed solut...

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Abstract

The invention discloses a method for extracting small molecule humic acid in soil by XAD resin, comprising the following steps: step a: soil treatment, step b: sodium pyrophosphate extracting soil humic acid, step c: sodium hydroxide extracting soil humic acid acid, step d: removal of fulvic acid impurities in humic acid, step e: separation of macromolecule humic acid and small molecule humic acid, step f: removal of silicon-containing minerals in small molecule humic acid, step g : Enrichment and extraction of small molecule humic acid. The method of the invention can well separate and extract small molecule humic acid in soil, and provide sample guarantee for comprehensively understanding the environmental behavior research of humic acid.

Description

technical field [0001] The invention relates to the extraction of organic matter in soil, in particular to a method for extracting small molecule humic acid in soil by using XAD resin. Background technique [0002] Humic acid is a macromolecular organic mixture formed by animal and plant residues through complex physical, chemical, and biological processes. It widely exists in environmental media such as soil, water, and sediment. Humic acid can be dissolved in alkaline and neutral solution, insoluble in acid accounts for about 60-80% of the total soil organic matter, and is the most important component of soil organic matter. [0003] Small molecule humic acid refers to humic acid with a molecular weight less than 10,000 Daltons. There are two schools of thought among researchers on the geochemical effects of small molecule humic acid. One school of researchers believes that the structure of small molecule humic acid is relatively simple, and the degree of humification of...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N1/34
Inventor 吴丰昌白英臣王海燕赵玉杰廖海清陈曲宋凡浩
Owner CHINESE RES ACAD OF ENVIRONMENTAL SCI
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