A method for comprehensive utilization of pleuromutilin waste bacteria residue resources

A technology of waste and recycling of pleuromutilin, which is applied in the field of resource recycling, can solve the problems of increasing secondary pollution, increasing floor area, management confusion, etc., and achieves the effects of improving economic benefits, increasing utilization rate, and increasing potency

Active Publication Date: 2018-08-07
山东胜利生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, various enterprises dry and seal the waste fungus residue, which not only increases the area occupied, but also increases the possibility of secondary pollution, and the cost is greatly increased; there are also companies that sell the waste fungus residue to other private companies for drying, processing and composting Fermentation to make organic fertilizer, but the management of this method is chaotic; some private companies directly make the fungus residue into feed additives, which has many potential safety hazards

Method used

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  • A method for comprehensive utilization of pleuromutilin waste bacteria residue resources

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] 1) Weigh 800 g of pleuromutilin waste slag with a water content below 2%, add 4Kg of water to the pleuromutilin waste slag, let it stand for 15 minutes, then add 4mol / L phosphoric acid to adjust the pH value to 5.0, to obtain Fungus residue liquid;

[0043] 2) Add 0.08 g of compound enzyme to the bacterial residue liquid obtained in 1), the compound enzyme is composed of cellulase: xylanase: β-glucanase in a mass ratio of 1:1:1, at 30°C Decompose for 60 minutes, filter to remove undecomposed residue, and the filtrate is protein nutrient solution;

[0044] 3) Take the strain and inoculate it into the seed medium, the inoculum size is 1cm 2 The slant was cultivated at 25.5°C for 65 hours, the pH value of the seed medium was 6.15, the ventilation rate was 1.0vvm, and the rotation speed was 220rpm.

[0045] The classification of the strain is named as clitopilus prunulus, and the Latin literary name is clitopilus prunulus. It is preserved in the General Microbiology Cente...

Embodiment 2

[0054] 1) Weigh 1.1Kg of pleuromutilin waste residue with a water content of less than 2%, add 5.5Kg of water to the waste pleuromutilin residue, let it stand for 15 minutes, then add 4mol / L phosphoric acid to adjust the pH to 5.0 , to obtain the slag liquid;

[0055] 2) Add 0.11 g of compound enzyme to the bacterial residue liquid obtained in 1), the compound enzyme is composed of cellulase: xylanase: β-glucanase in a mass ratio of 1:1:1. Decompose for 70 minutes, filter to remove undecomposed residue, and the filtrate is protein nutrient solution;

[0056] 3) Take the strains and inoculate them into the seed culture medium, the inoculum size is 1.5cm 2 The slant was cultivated at 26°C for 68 hours, the pH of the seed medium was 6.2, the ventilation rate was 1.1vvm, and the rotation speed was 230rpm.

[0057] The classification of the strain is named as clitopilus prunulus, and the Latin literary name is clitopilus prunulus. It is preserved in the General Microbiology Cente...

Embodiment 3

[0066] 1) Weigh 50Kg of pleuromutilin waste slag with a water content below 2%, add 250Kg of water to the pleuromutilin waste slag, let it stand for 15min, then add 4mol / L phosphoric acid to adjust the pH value to 5.0, to obtain Fungus residue liquid;

[0067] 2) Add 5 g of compound enzyme to the bacterial residue liquid obtained in 1), the compound enzyme is composed of cellulase: xylanase: β-glucanase in a mass ratio of 1:1:1, and enzymatically hydrolyzed at 30°C 90min, filter to remove undecomposed residue, the filtrate is protein nutrient solution;

[0068] 3) Get the strain and inoculate it into the seed culture medium, the inoculum size is 2cm 2 The inclined plane was cultivated at 26.5°C for 70 hours, the pH value of the seed medium was 6.25, the ventilation rate was 1.1vvm, and the rotation speed was 250rpm.

[0069] The classification of the strain is named as clitopilus prunulus, and the Latin literary name is clitopilus prunulus. It is preserved in the General Mic...

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Abstract

The invention relates to a method for comprehensive utilization of pleuromutilin waste bacterial residue as a resource, comprising: taking pleuromutilin waste bacterial residue with a water content of less than 2%, adding water, standing for 15 minutes, and adding 4mol / L phosphoric acid to adjust pH When the value is 5.0-6.0, the bacterial residue liquid is obtained; add compound enzymes to the bacterial residue liquid, enzymatically hydrolyze for 60-90min at 30°C, filter, and the filtrate is a protein nutrient solution; take the strains, and classify the strains and name them Umbrella oblique, Latin literature The name is clitopilus prunulus, and it was preserved in the General Microorganism Center of China Microbiological Culture Collection Management Committee. The preservation date was May 8, 2012, and the preservation number was CGMCC No.6093. 6.15-6.25, the ventilation rate is 1.0-1.1vvm, the rotation speed is 200-250rpm, inoculate the cultured strains into the fermentation medium, and the inoculation amount of the strains accounts for 8%-10 of the total mass of the strains and the fermentation medium %, cultured at 24.5-26.5°C for 270-300h, the pH value of the fermentation medium was 8.15-8.25, the ventilation rate was 0.6-1.3vvm, and the rotation speed was 70-115rpm to obtain a fermentation broth.

Description

technical field [0001] The invention relates to a resource comprehensive utilization method of waste pleuromutilin residue and belongs to the field of resource recycling. Background technique [0002] The waste pleuromutilin residue is the residue left after the fermentation of pleuromutilin, mainly including mycelia, remaining medium, inorganic salts, metabolites and a small amount of pleuromutilin, etc. After testing, the waste fungus residue is rich in protein, and the content can reach about 20%. In addition to protein, the fungus residue also contains relatively rich polysaccharides, fats, starch, cellulose and other substances. According to the provisions of the National Hazardous Waste Catalogue: the waste bacterial residue produced by the fermentation of pleuromutilin belongs to hazardous waste, code HW-02. According to regulations, the treatment of hazardous waste must be handed over to a qualified formal unit for treatment. The treatment fee per ton is about 3,000...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P15/00C12R1/645
Inventor 邱向东张铜镇何惠欣张善飞王甜
Owner 山东胜利生物工程有限公司
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