Method for preparing high-purity xylo-oligosaccarides by taking cottonseed hulls as raw materials

Abstract

The invention discloses a method for preparing high-purity xylo-oligosaccarides by taking cottonseed hulls as raw materials. In the xylo-oligosaccaride preparation process by taking the cottonseed hulls as the raw materials, the high-temperature steaming time and temperature in the pretreatment process are regulated, the xylose content in steaming liquor is controlled, the xylanase adding amount and the enzymolysis time in the enzymolysis process are controlled, and the xylo-oligosaccarides with the purity larger than or equal to 90 percent are prepared. The method has the advantages that the purity of the prepared xylo-oligosaccarides is larger than or equal to 90 percent, and the separation and purification steps such as membrane separation and moving bed simulation in a traditional high-purity xylo-oligosaccaride preparation technology are omitted; the xylo-oligosaccarides are prepared through the cottonseed hulls, the xylanase adding amount accounts for 0.01%-0.2% of the dry weight of the cottonseed hulls, the enzymolysis time is set for 6-10 h, and the xylanase using amount in the prior art is decreased, and the enzymolysis time in the prior art is shortened. According to the method, the cost is reduced, the efficiency is improved, and the product quality is promoted.

Description

technical field [0001] The invention relates to a preparation method of xylooligosaccharides, in particular to a method for efficiently preparing high-purity xylooligosaccharides from cottonseed hulls, and belongs to the technical field of functional sugar preparation. Background technique [0002] Xylo-oligosaccharides are a kind of oligosaccharides, which are formed by β-1,4-endoxylanase with xylan as a substrate and hydrolyze β-1,4 glycosidic bonds with a degree of polymerization of 2-7 of oligosaccharides. Xylo-oligosaccharides can improve the balance of the digestive tract flora of organisms (humans and animals), promote the growth of beneficial bacteria in the digestive tract, inhibit the reproduction of harmful microorganisms, promote nutrient absorption, and improve the body's immunity. In recent years, high-purity xylo-oligosaccharides are in increasing demand due to their more prominent efficacy and higher added value. [0003] Cottonseed hulls are mainly compose...

AI Technical Summary

Problems solved by technology

Although these methods have improved the purity of xylooligosaccharides, the separation process inevitably results in the loss of sugar solution, and requires a large amount of pure water. Increased cost of xylose preparation

[0006] Patent CN1333371A mentions that a kind of bagasse is used as raw material to prepare crude xylan by alkali dissolution and alcohol precipitation, and then directional enzymolysis with xylanase. The purity of the prepared xylooligosaccharides is 92.9%. Up to 24 hours, which affects the preparation efficiency; in addition, because ethanol is flammable, a specific environment is required during use

Patent CN101565468B uses cottonseed hulls as raw materials to prepare xylo-oligosaccharides, but the amount of xylanase added is as high as 1.0-2.5% of the dry weight of cottonseed hulls, and the enzymatic hydrolysis time is as long as 24-36 hours, which affects the cost of cottonseed hulls xylo-oligosaccharides and efficiency, in addition, the content of xylooligosaccharide in the prepared product is about 80%

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