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High-strength polypeptide hydrogel preparation method

A hydrogel, high-strength technology, used in non-active medical preparations, pharmaceutical formulations, non-active components of polymer compounds, etc. problems, to achieve the effect of improving the mechanical strength and improving the ability to form glue

Active Publication Date: 2016-01-27
CHINA UNIV OF PETROLEUM (EAST CHINA)
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

This process is based on the formation of non-covalent interactions, the ability to form gels is limited, and the cross-linking strength between fibers is often low, resulting in limited gel strength, poor self-supporting performance, and easy to be destroyed; moreover, its fiber cross-linking The pore size of the three-dimensional network structure formed by the association is often small and difficult to effectively control, which greatly limits its application in the field of tissue engineering materials.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] (1) Preparation I 3 K polypeptide molecular 4.0mmol / L aqueous solution, ultrasonic mixing, ultrasonic power 100W, ultrasonic time 10min; stand at room temperature for 3 days to make it self-assemble into a long fiber structure, at this time the system can not form a hydrogel;

[0036] (2) Add GO sheets with a length of 300±200nm, a width of 300±200nm, and a thickness of 1-2nm in the solution containing the polypeptide long fiber structure, so that the concentration of the GO sheet is 0.1 mg / mL (that is, every mL Add 0.1 mg GO flakes to the solution), and ultrasonically mix, the ultrasonic power is 100W, and the ultrasonic time is 12min; the pH value of the mixed solution is adjusted to 10.5 by using NaOH solution with a concentration of 0.5mol / L, and it is allowed to stand at room temperature for 6 hours to form hybrid water gel.

[0037]The storage modulus (G') of the hydrogel obtained in Example 1 can reach 4000Pa, the energy dissipation modulus (G") can reach 500Pa,...

Embodiment 2

[0039] (1) Prepare K 3 A 3 I 3 G 3 V 3 Polypeptide molecule 4.0mmol / L aqueous solution, ultrasonic mixing, ultrasonic power 100W, ultrasonic time 10min; stand at room temperature for 3 days to make it self-assemble into a long fiber structure;

[0040] (2) Add GO sheets with a length of 300±200nm, a width of 300±200nm, and a thickness of 1-2nm to the above-mentioned long fiber structure solution, so that the concentration of the GO sheet is 0.02mg / mL, ultrasonically mix, and the ultrasonic power 100W, ultrasonic time is 10min; use 0.5mol / L NaOH solution to adjust the pH of the mixed solution to 10.0, and let it stand at room temperature for 6 hours to form a hybrid hydrogel.

[0041] The storage modulus (G') of the hydrogel obtained in Example 2 can reach 5000Pa, the energy dissipation modulus (G") can reach 600Pa, and the gel pore size is less than 500nm; the self-supporting hybrid Hydrogels.

Embodiment 3

[0043] (1) Prepare K 3 A 3 I 3 G 3 V 3 Polypeptide molecule 4.0mmol / L aqueous solution, ultrasonic mixing, ultrasonic power 100W, ultrasonic time 10min; stand at room temperature for 3 days to make it self-assemble into a long fiber structure;

[0044] (2) Add GO sheets with a length of 300±200nm, a width of 300±200nm, and a thickness of 1-2nm to the above-mentioned long fiber structure solution, so that the concentration of the GO sheet is 0.1mg / mL, ultrasonically mix, and the ultrasonic power 100W, ultrasonic time is 10min; use 0.5mol / L NaOH solution to adjust the pH of the mixed solution to 10.0, and let it stand at room temperature for 6 hours to form a hybrid hydrogel.

[0045] The storage modulus (G') of the hydrogel obtained in Example 3 can reach 6000Pa, the loss modulus (G") can reach 1000Pa, and the gel pore size is less than 500nm. image 3 It is the rheological measurement result of the hybrid hydrogel of this embodiment; a self-supporting hybrid hydrogel can ...

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Abstract

The invention relates to a high-strength polypeptide hydrogel preparation method and belongs to the field of chemical preparation. The high-strength polypeptide hydrogel preparation method includes (1) selecting polypeptide molecules, which are provided with positive charges and capable of forming long fiber structures by self-assembly, to prepare an aqueous solution, mixing uniformly ultrasonically and standing so as to enable the aqueous solution to form the long fiber structures by self-assembly; (2) adding GO (graphene oxide) lamellas into the solution of the long fiber structures, mixing uniformly ultrasonically, regulating a pH (potential of hydrogen) value, standing and forming hybrid hydrogel after a system is aged and stabilizes. The high-strength polypeptide hydrogel preparation method has the advantages that the GO lamellas are introduced into the polypeptide hydrogel, and gelling capability of the system is improved, so that a high-strength polypeptide / GO hybrid hydrogel material is obtained; properties of mechanical strength, aperture size and the like of the polypeptide / GO hybrid hydrogel can be controlled by controlling the size, surface charge density and surface charge concentration of the GO lamellas.

Description

technical field [0001] The invention relates to a preparation method of a hydrogel, in particular to a preparation method of a high-strength polypeptide hydrogel; it belongs to the field of chemical preparation. Background technique [0002] Hydrogel is a gel material with water as the dispersion medium. Due to its rich pore structure and three-dimensional network structure that can provide better permeability and mechanical support, it has broad application prospects in many fields. In recent years, hydrogel materials have attracted the attention of scientists at home and abroad, and a lot of research has been done on their applications in biomedicine, such as tissue engineering and drug delivery. Ordinary hydrogels can be obtained by covalent cross-linking between high-molecular-weight synthetic polymers or between some small molecules, but such cross-linking methods need to be carried out by chemical synthesis. Since most of the chemical reagents are toxic, the hydrogel ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/24A61L27/08A61L27/52A61L27/56A61K9/06A61K47/42A61K47/04
Inventor 曹美文徐海汪蕾王继乾王栋吕建仁
Owner CHINA UNIV OF PETROLEUM (EAST CHINA)
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