Ginger stem rot antagonistic bacterium and applications
A technology for root rot and germs of ginger stems, applied in the directions of application, bacteria, biocides, etc.
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Embodiment 1
[0031] Example 1: Obtaining and identifying the antagonistic bacteria C3 of ginger stem rot fungus
[0032] Antagonistic bacteria were screened from the rhizosphere of scallions, and the screened antagonistic bacteria were identified by morphological and molecular biology methods.
[0033] (1) Morphological identification:
[0034] Bacterial morphology is relatively stable under appropriate conditions. Streak the screened antagonistic bacteria on NA medium and observe the characteristics of single colony after 24 hours of culture, including colony shape, size, color uniformity, bacterial odor, and whether the medium has changed color. etc. for preliminary identification, the results are as follows figure 1 shown;
[0035] (2) Molecular biological identification:
[0036] Through 16SrDNA sequencing and RecA gene sequencing, the sequence of the sequencing result was compared with the submitted sequence in GenBank, and the strain C3 was identified as Burkholderia cepacia (Burk...
Embodiment 2
[0038] Embodiment 2: antibacterial effect
[0039] (1) The inhibitory effect of antagonistic bacteria C3 on the pathogenic bacteria of ginger stem rot
[0040] Inhibitory effect of C3 on root rot pathogen of ginger stem, such as figure 2 As shown, there is C3 in the middle of the petri dish, surrounded by ginger stem rot fungus, therefore, C3 obviously has inhibitory effect on ginger stem root rot fungus. Such as image 3 As shown, among them, picture A is the control, and the mycelium is smooth in surface, uniform in thickness, and tubular in shape; picture B is the mycelium after 2 days of confrontation culture between C3 and the root rot pathogen of ginger, showing local swelling of the mycelium , Abnormal branching and other abnormal growth.
[0041] (2) Inhibitory effect of antagonistic bacteria C3 of ginger stem rot pathogen on indicator pathogens
[0042] Such as Figure 4 , 5 As shown, C3 showed obvious inhibitory effect on stem rot fungus, R. solanacearum and R...
Embodiment 3
[0043] Example 3: Indoor control effect of ginger stalk rot pathogen antagonistic bacteria C3 on stalk rot
[0044] (1) Preparation of biocontrol agent and pathogenic inoculum
[0045] Bacterial suspension preparation: Cultivate the antagonistic bacterium C3 of Ginger stem rot fungus with optimized medium, when the 600nmOD value is 1, centrifuge the bacterial suspension at 6000rpm for 10min, resuspend the bacteria with an equal volume of sterile water or phosphate buffer, and dilute to Bacteria concentration is 5×10 8 cfu / ml, after aliquoting, store at 4°C for later use.
[0046] Auxiliary carrier: mix the three substances according to the ratio of 60% maltodextrin, 10% yeast powder and 30% wheat bran.
[0047] Pathogen inoculum: Inoculate stem rot pathogens into sterilized wheat grains, culture at 28°C for 7 days, and set aside. See Table 1 for details of the experimental design.
[0048] Table 1 Experimental design and different treatments
[0049]
[0050] First, mix t...
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