Application of ginseng pectin in resisting depressive behavior

An antidepressant and pectin technology, which can be applied to medical preparations containing active ingredients, plant raw materials, nervous system diseases, etc., can solve the problems of the active components and molecular mechanisms of antidepressants that have not been verified yet.

Inactive Publication Date: 2016-02-03
JIANGSU UNIV
View PDF1 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The inventor's previous experimental results have proved that ginseng pectin WGPA can significantly reverse the fixed time of the forced swimming test in mice, and has antidepressant activity (Wang, J, Flaisher-Grinberg, S., Li, S.S., Liu, H.B., Sun, L.,Zhou,Y.F.,etal.(2010).Antidepressant-like effect of the active acidic polysaccharide portion of ginsenginmice.Journal of Ethnopharmacology,132,65-69.), however, its antidepressant active components and molecular mechanism have not yet been verified

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of ginseng pectin in resisting depressive behavior
  • Application of ginseng pectin in resisting depressive behavior
  • Application of ginseng pectin in resisting depressive behavior

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Embodiment 1: the preparation of ginseng pectin

[0026]Preparation of Ginseng Pectin WGPA:

[0027] The extraction and separation process is as follows: ginseng root (commercially available) is boiled and extracted in hot water, ginsenosides are separated from the macroporous resin, and the residue is alcohol-precipitated to obtain crude polysaccharides. Crude polysaccharides were deproteinized by Sevag method and refined by alcohol precipitation to obtain total polysaccharides WGP (Watersolubleginsengpolysaccharides). WGP was separated into amyloid polysaccharide WGPN (Starch-likeglucans) and ginseng pectin WGPA (Ginsengpectin) by DEAE-Cellulose ion exchange column. WGPA was redissolved in water, separated by DEAE-Cellulose ion exchange column, and gradient eluted with NaCl aqueous solution (0M, 0.5M), so as to obtain the neutral component WGPA-N and acidic component WGPA-A of ginseng pectin.

Embodiment 2

[0028] Embodiment 2: Behavioral experiment

[0029] BlackSwiss mice were purchased from Jiangsu University, aged 10-12 weeks, and divided into three groups, 10 mice in each group. The control group was the normal saline group, and the administration volume was 10mL / kg; kg group and 200mg / kg group.

[0030] (1) Opening autonomous activity experiment

[0031] On day 8 after dosing, mice were housed in a 50 cm x 25 cm x 20 cm transparent automatic activity monitor (OptoM3, Columbus Instruments, Columbus, OH). Infrared monitoring was recorded once every 10 minutes, and the autonomous activities of the mice were continuously recorded 6 times for a total of 60 minutes. After the experiment was completed, the mice were sent back to the breeding cage, and the monitor was wiped clean with 10% alcohol.

[0032] The results of the opening autonomous activity experiment are shown in Table 1. Whether in the first 10 minutes or within 60 minutes, WGPA-A did not affect the autonomous a...

Embodiment 3

[0046] Example 3: Mechanism studies

[0047] Preparation of brain tissue: On the 12th day of administration, mice were sacrificed by CO2 anesthesia overdose 1 hour after administration, and the hippocampus was placed in liquid nitrogen and transferred to a -80°C refrigerator. Homogenization: Brain tissue samples were added to 9× volume of 50mM KPO4 buffer, 0.5% Triton-X-100 pH7.2 and 1× EDTA-free protease inhibitor, homogenized using Barnant homogenizer, brain homogenate Centrifuge at 13,000 g for 15 min at 4°C, take the supernatant and store it in a -80°C refrigerator, and use the BCA protein assay kit to detect the protein concentration.

[0048] β-catenin Western Blotting analysis: Beta-catenin was loaded at 1 μg / well, loaded on 8% gel, electrophoresed on SDS-PAGE (100V, 1h), separated protein was transferred to PVDF membrane, 5% delipidated Blocked with milk powder for 1 hour, incubated with rabbit-derived Anti-betacatenin polyclonal primary antibody (1:5000, Abcam, Inc...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to application of ginseng pectin in resisting depressive behavior, and belongs to the field of traditional Chinese medicine application. Ginseng pectin is given to a mouse for 12 days in force feeding manner, and the activity of resisting a depressive behavior is evaluated by an open-field locomotor activity test, an elevated plus maze test, a social interference test and a forced swimming test. Results indicate that by using the ginseng pectin, the interference time and frequency in a mouse social interference test can be increased, the invasion time and frequency of the mouse can be reduced, the time rate and frequency rate of an open-arm of the mouse entering an elevated plus maze can be increased, the standing time of the mouse forced swimming test can be reduced, and protein expression of hippocampus beta-catenin and brain-derived neurotrophic factor (BDNF) of the mouse can be increased along with changes of the behaviors, namely the ginseng pectin can be used for enhancing release of transmitters among synapses and connection among synapses by inducing the protein expression of hippocampus beta-catenin and BDNF so as to influence the neuron plasticity and improve neuron development and achieve the activity of resisting the depressive behavior.

Description

technical field [0001] The invention relates to the application of ginseng pectin in antidepressant-like behavior, belonging to the application field of traditional Chinese medicine. Background technique [0002] Ginseng has been known for strengthening the body and prolonging life since ancient times. It has the effects of anti-fatigue, anti-oxidation, regulating substance metabolism, and regulating the central nervous system. The neuroprotective effects of ginseng and its extracts have been widely confirmed. For example, ginseng antagonizes Parkinson's disease through a neuroprotective mechanism (VanKampen, J.M., Baranowski, D.B., Shaw, C.A., & Kay, D.G. (2014). PanaxginsengisneuroprotectiveinanovelprogressivemodelofParkinson'sdisease. 50,95-105.), in addition, its extracts such as ginsenoside Rg3 have also been shown to antagonize 24-hydroxy-cholesterol-induced cytotoxicity and protect cortical neurons (Roh, Y.S., Kim, H.B., Kang, C.W. , Kim, B.S, Nah, S.Y, & Kim, J.H. (...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/258A61K31/732A61P25/24A61P25/22
Inventor 王佳张维宁王玉聪陈嘉欣鲁京欣査利晨宗慧敏王文欣彭颢陈宇璇周露周甜甜侯卓然
Owner JIANGSU UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap