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Chip for polymerase chain reaction (PCR), and real-time detection device and system

A real-time detection and chip technology, used in enzymology/microbiology devices, bioreactors/fermenters for specific purposes, biochemical cleaning devices, etc. and other issues to achieve the effect of improving convenience, ensuring consistency, and being easy to carry.

Inactive Publication Date: 2016-02-03
PEKING UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to overcome the shortcomings of the complex structure of the current fluorescent quantitative PCR instrument and the difficulty in real-time monitoring of the electrochemical chip, the present invention proposes a new polymerase chain reaction chip and its real-time detection device and system

Method used

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  • Chip for polymerase chain reaction (PCR), and real-time detection device and system
  • Chip for polymerase chain reaction (PCR), and real-time detection device and system
  • Chip for polymerase chain reaction (PCR), and real-time detection device and system

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Embodiment 1

[0023] In this example, if figure 1 and 2 Shown is a schematic structural view of the PCR chip of the present invention, including: a thermal cover 1, an injection joint plate 2, an inlet and outlet orifice plate 3, a reaction pool plate 4, a stopper 5, an electrochemical substrate 6 and a temperature-adjusting base 7, Wherein: heat cover 1 completes the heating of the top layer of the chip; under heat cover 1 is injection joint plate 2, and injection joint plate 2 is provided with a plurality of reagent injection joints, which are docked with the microfluidic module of the external real-time detection device; The positions of the injection joints are corresponding, and there are reagent inlet and outlet holes on the inlet and outlet orifice plate 3; each group of inlet and outlet holes corresponds to a reaction cell arranged on the reaction cell plate 4; The substrate 6 has a reaction surface and a circuit connection point, and a PCR reaction area corresponding to the reacti...

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Abstract

The invention discloses a chip for a polymerase chain reaction (PCR), and a real-time detection device and system. The PCR chip disclosed by the invention adopts a multilayer structure and is connected with a temperature control module, a microfluidic control module, an ultra-weak electronic signal detection module and a constant-voltage source module of the detection device; PCR temperature control, microfluidic control and weak signal acquisition and measurement are finished on the miniature chip to realize real-time electrochemical detection of PCR amplification; light source and photoelectric conversion is not involved; real-time monitoring is performed in a reaction process to avoid pollution. The PCR chip and the real-time detection device thereof are small in size and convenient to carry and are easily promoted and used in a basic level.

Description

technical field [0001] The invention relates to a polymerase chain reaction (PCR) chip and a real-time detection device, in particular to an electrochemical-based biological chip and a weak current detection device for the chip. Background technique [0002] Polymerase Chain Reaction (Polymerase Chain Reaction, referred to as PCR), is a method of enzymatically synthesizing specific DNA fragments in vitro. DNA can be rapidly amplified, and has the characteristics of strong specificity, high sensitivity, easy operation, and time saving. It can not only be used in basic research such as gene isolation, cloning and nucleic acid sequence analysis, but also in medical, food and health fields such as pathogen detection, prenatal diagnosis, tumor gene detection, efficacy evaluation, food safety detection, and food genetically modified detection. [0003] Real-time fluorescence quantitative PCR (QuantitativeReal-timePCR), during the PCR amplification process, by adding fluorescent s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/38C12M1/36C12M1/34G01N27/26
CPCB01L7/52G01N27/26
Inventor 陆祖宏李清宁严勇孙利魏颖颖李成强孙杰王磊
Owner PEKING UNIV