Method for controlling biological safety of pickled vegetable
A microbial and safety technology, applied in microbial-based methods, microbial determination/inspection, microorganisms, etc., can solve problems such as spoilage, food-borne diseases, human health, and impact, and achieve simple steps, simple and effective microbial safety. The effect of shortening the experiment time
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[0019] Homemade 3 kinds of kimchi with different salt concentration: the raw material is Chinese cabbage, the mass ratio of vegetable and water is 1:1, the salt concentration is 2%, 5%, and 10% respectively (numbers are 1, 2, 3), room temperature Ferment for 6 days; buy a bag of kimchi near the expiration date (number 4). Cultivate 4 kinds of pathogenic bacteria Salmonella, Staphylococcus aureus, Escherichia coli, and Listeria monocytogenes. Salmonella, Staphylococcus aureus, and Escherichia coli were expanded with nutrient broth medium, and Listeria monocytogenes was grown with brain heart infusion (BHI) broth medium. 10 per bacteria 6 -10 7 The amount of CFU / g was mixed and inoculated into 4 parts of kimchi. Place it at room temperature, and measure the viable counts of the 4 pathogenic bacteria on the 3rd and 6th days.
[0020] The detection methods of the four pathogenic bacteria are as follows: The detection method of coliforms refers to the national standard GB47893-2010 ...
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