A method of culturing pearls by using pearl nucleuses with tissue engineering scaffolds

A tissue engineering scaffold and bead core technology, applied in the field of nucleated pearl culture, can solve the problems of pearl shape, uneven color, low cell volume, cell-free area, etc., to avoid high technical and equipment requirements and easy operation. Effect
CN105309346AInactive Publication Date: 2016-02-10FUJIAN NORMAL UNIV
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
FUJIAN NORMAL UNIV
Publication Date
2016-02-10
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention provides a method of culturing pearls by using pearl nucleuses with tissue engineering scaffolds. The method is characterized in that a layer of degradable high-molecular polymer with a uniform thickness, serving as a tissue engineering scaffold, is attached to the surface of a pearl nucleus which is to be implanted into a pearl mussel; the tissue engineering scaffold is three-dimensional network space capable of accommodating and growing cells; mantle tissue of pearl shells is separated into single existent free mantle cells by using the enzyme digestion method and a mantle cell suspension with a certain cell density is prepared; mantle cells are attached to the pearl nucleus and then the pearl nucleus and the mantle cell suspension are implanted in a nucleus insertion position of the pearl mussel for culture of a nucleus pearl. The method is simple in operation, requires no in-vitro culture of cells and solves the problem of high technology and equipment requirements of a cell culture method; aseptically packaged pearl nucleuses can be directly transported to culture sites for operation and the transportation is convenient; the method has low requirements for equipment and operators, reduces the production cost greatly and is suitable for large-scale production and application.
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Description

technical field

[0001] The invention relates to a method for cultivating nucleated pearls, in particular to the application of a bead nucleus with a tissue engineering support adhered to the surface in the cultivation of nucleated pearls. Background technique

[0002] Tissue engineering scaffold is a synthetic framework with three-dimensional structure, which simulates the function of extracellular matrix in body tissue, so that cells can adhere, migrate and proliferate on this three-dimensional structure, and finally the tissue can be reconstructed. Materials commonly used to prepare tissue engineering scaffolds include bioactive ceramics and degradable polymer materials. Degradable polymer materials can be divided into natural polymer materials and synthetic polymer materials.

[0003] At present, the cultivation of nucleated pearls usually all implants the nucleus inserting position of the pearl clam with the mantle cell sheet and the pearl core, and then puts it into th...

Claims

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