A method of culturing pearls by using pearl nucleuses with tissue engineering scaffolds

A tissue engineering scaffold and bead core technology, applied in the field of nucleated pearl culture, can solve the problems of pearl shape, uneven color, low cell volume, cell-free area, etc., to avoid high technical and equipment requirements and easy operation. Effect

Inactive Publication Date: 2016-02-10
FUJIAN NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method has the following three problems: ⑴ epidermal epithelial cells are the only cells that can attach to the surface of the pearl nucleus, but the formation of pearl sacs also requires connective tissue cells from the mantle (reference 3); Under culture conditions, it can only grow into a monolayer by adherence, and the amount of cells is too small
(3) This method also has the problem that cells cannot be attached to the contact surface between the bead nucleus and the cu

Method used

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  • A method of culturing pearls by using pearl nucleuses with tissue engineering scaffolds
  • A method of culturing pearls by using pearl nucleuses with tissue engineering scaffolds
  • A method of culturing pearls by using pearl nucleuses with tissue engineering scaffolds

Examples

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Effect test

Embodiment 1

[0039] 1. Autoclave the polished pearl nuclei, put them into 0.01wt% polylysine solution, shake and soak for 30 minutes, take them out, and dry them under aseptic conditions.

[0040] 2. Preparation of clam serum: extract blood from the blood sinus of Pinctada martensii, centrifuge at 2000r / min for 10 minutes, take the supernatant, put it in a 56°C water bath for 30 minutes, and then pass the obtained serum through 0.2μm Filtered with a membrane filter, prepared with PBS to a concentration of 10vol%, and stored at 4°C for later use.

[0041] 3. Preparation of bead-core tissue engineering scaffold layer mold: Mold Ⅰ is composed of two hollow hemispheres with the same structure, and when used, the two hemispheres are closed to form a complete sphere. On the inner surface of each hemisphere, there are 3 strip-shaped protrusions (hereinafter referred to as ribs) arranged in the direction of the north and south poles (the location of the glue injection hole is the south pole, and t...

Embodiment 2

[0052] 1. Autoclave the polished pearl nuclei, put them into 0.1wt% polylysine solution, shake and soak for 10 minutes, take them out, and dry them under aseptic conditions.

[0053] 2. Preparation of clam serum: extract blood from the blood sinus of Pinctada martensii, centrifuge at 2000r / min for 10 minutes, take the supernatant, put it in a 56°C water bath for 30 minutes, and then pass the obtained serum through 0.2μm Filtered with a membrane filter, prepared with PBS to a concentration of 10vol%, and stored at 4°C for later use.

[0054] 3. Preparation of bead-core tissue engineering scaffold layer mold: Mold Ⅰ is composed of two hollow hemispheres, each hemisphere has three raised edges on the inner surface, the length of the edges is slightly shorter than the circumference of the hemisphere, and two of the edges are located at On the edge of the hemisphere, the other edge is located in the middle of the hemisphere, the middle edge is arranged in parallel with the edge edg...

Embodiment 3

[0065] 1. Autoclave the polished pearl nuclei, put them into 0.1wt% mouse-derived type I collagen solution, shake and soak for 30 minutes, take them out, and dry them under aseptic conditions.

[0066] 2. Preparation of clam serum: extract blood from the blood sinus of Pinctada martensii, centrifuge at 2000r / min for 10 minutes, take the supernatant, put it in a 56°C water bath for 30 minutes, and then pass the obtained serum through 0.2μm Filtered with a membrane filter, prepared with PBS to a concentration of 10vol%, and stored at 4°C for later use.

[0067] 3. Preparation of bead-core tissue engineering scaffold layer mold: Mold Ⅰ is composed of two hollow hemispheres, each hemisphere has three raised edges on the inner surface, the length of the edges is slightly shorter than the circumference of the hemisphere, and two of the edges are located at On the edge of the hemisphere, the other edge is located in the middle of the hemisphere, the middle edge is arranged parallel t...

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Abstract

The invention provides a method of culturing pearls by using pearl nucleuses with tissue engineering scaffolds. The method is characterized in that a layer of degradable high-molecular polymer with a uniform thickness, serving as a tissue engineering scaffold, is attached to the surface of a pearl nucleus which is to be implanted into a pearl mussel; the tissue engineering scaffold is three-dimensional network space capable of accommodating and growing cells; mantle tissue of pearl shells is separated into single existent free mantle cells by using the enzyme digestion method and a mantle cell suspension with a certain cell density is prepared; mantle cells are attached to the pearl nucleus and then the pearl nucleus and the mantle cell suspension are implanted in a nucleus insertion position of the pearl mussel for culture of a nucleus pearl. The method is simple in operation, requires no in-vitro culture of cells and solves the problem of high technology and equipment requirements of a cell culture method; aseptically packaged pearl nucleuses can be directly transported to culture sites for operation and the transportation is convenient; the method has low requirements for equipment and operators, reduces the production cost greatly and is suitable for large-scale production and application.

Description

technical field [0001] The invention relates to a method for cultivating nucleated pearls, in particular to the application of a bead nucleus with a tissue engineering support adhered to the surface in the cultivation of nucleated pearls. Background technique [0002] Tissue engineering scaffold is a synthetic framework with three-dimensional structure, which simulates the function of extracellular matrix in body tissue, so that cells can adhere, migrate and proliferate on this three-dimensional structure, and finally the tissue can be reconstructed. Materials commonly used to prepare tissue engineering scaffolds include bioactive ceramics and degradable polymer materials. Degradable polymer materials can be divided into natural polymer materials and synthetic polymer materials. [0003] At present, the cultivation of nucleated pearls usually all implants the nucleus inserting position of the pearl clam with the mantle cell sheet and the pearl core, and then puts it into th...

Claims

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Application Information

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IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 林尧肖义军黄义德陈元仲王正朝肖宽诚
Owner FUJIAN NORMAL UNIV
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