The invention aims at providing a radiopharmaceutical automatic split charging process method and system based on an industrial robot. The system mainly consists of a split charging heat chamber, a control system, an industrial robot, an end picker module, a feeding and discharging module, a radiopharmaceutical tank bracket module, an activity detection module, a protection combination bracket module and an injector discharging module. The system uses the industrial robot as a core for connecting all work procedures for the radiopharmaceutical split charging in series, and the work links such as the automatic material feeding and discharging, injector protection sleeve and injector needle cap detaching, penicillin bottle radiopharmaceutical total activity detection, needle insertion and liquid extraction, injector activity detection, injector protection sleeve installation and injector output are realized. The system has the advantages that the automatic split charging and detection technical process of radiopharmaceuticals can be accurately and reliably completed, the work mode conforms to relevant industrial standards, the artificial participation is avoided, the liquid extraction precision is high, no bubble or liquid residue exists, and the use of purpose-made high-cost consumables and the detection and the processing of residue radiopharmaceuticals are avoided.