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Bactericidal composition containing bacillus subtilis and kresoxim-methyl

A technology of Bacillus subtilis and kresoxim-methyl, which is applied in the direction of fungicides, biocides, biocides, etc.

Inactive Publication Date: 2016-02-24
海南博士威生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

And there is no report about the compounding of kresoxystrobin and Bacillus subtilis at home and abroad

Method used

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  • Bactericidal composition containing bacillus subtilis and kresoxim-methyl
  • Bactericidal composition containing bacillus subtilis and kresoxim-methyl
  • Bactericidal composition containing bacillus subtilis and kresoxim-methyl

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example One Bacillus subtilis XM16 (Bacillus subtilis XM16) was cultured with NA medium (beef extract 3.0g, yeast extract 1g, peptone 5g, glucose 10g, agar 20g, distilled water 1000mL) at 28°C for 2 days with shaking (150r / m), and set aside . Dilute 20% kresoxim-methyl 500×, 1000×, 2000× and put it in the NA medium, and spread the cultured Bacillus subtilis XM16 diluted 109 times in the prepared NA medium, set clear water as the control, and make respectively Three repetitions were performed, and the experimental results are shown in Table 1.

[0033] Table 1

[0034]

[0035] Wherein the proliferation rate is an index for increasing the growth effect, and the proliferation rate=(the number of grown strains of the treated bacteria-the number of grown strains of the control bacteria) / the number of grown strains of the control bacteria×100%. The experimental results showed that kresoxystrobin could promote the growth of Bacillus subtilis XM16.

Embodiment 2

[0036] The preparation of embodiment Bacillus subtilis parent powder

[0037] Bacillus subtilis XM16 (Bacillus subtilisXM16) is cultivated on a slant, expanded on a shaker, fermented in a seed tank and fermented in a fermenter; 20L of the fermentation liquid of the strain is adsorbed with 120kg of medical stone in an adsorption stirring device, and then stirred evenly. Pulverize to obtain Bacillus subtilis mother powder, wherein the required bacterial content is 10-100 billion CFU / g.

Embodiment 3

[0038] Embodiment 3 The bactericidal wettable powder of Bacillus subtilis and kresoxim-methyl provided by the present invention is composed of the following components in weight percentage:

[0039] Bacillus subtilis mother powder 15%,

[0040] Kresoxim-methyl 15%,

[0041] Carrier 60.5%,

[0042] Dispersant 4.5%,

[0043] Wetting agent 2%,

[0044] Penetrant 3%.

[0045] Add 15 kg of Bacillus subtilis parent powder, 15 kg of kresoxim-methyl, 60.5 kg of kaolin, 4.5 kg of sodium lignosulfonate, 2 kg of dodecylbenzenesulfonic acid and 3 kg of fatty alcohol polyoxyethylene ether into the reaction kettle Stir evenly, take out the reactant after 30 minutes, put it into a pulverizer, pulverize, and sieve. The resultant is the bactericidal wettable powder of Bacillus subtilis and kresoxim-methyl.

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Abstract

The invention relates to bactericidal composition, in particular to bactericidal composition containing bacillus subtilis and kresoxim-methyl and a preparation method of the bactericidal composition. The bactericidal composition comprises components in percentage by weight as follows: 1%-25% of bacillus subtilis powder, 10%-30% of kresoxim-methyl and the balance of auxiliaries. The invention provides a bactericide containing bacillus subtilis and kresoxim-methyl. The bactericide effect can be improved, the use time of the bactericide can be prolonged, the use cost can be reduced, the synergistic interaction effect is remarkable, the control effect on diseases is good, and the bactericidal composition is mainly used for controlling diseases such as tomato fusarium wilt, tomato bacterial wilt, wheat scab, powdery mildew, tomato anthracnose, grape powdery mildew, botrytis, a soybean root rot disease, apple molding core, cotton damping-off, rice blast and the like.

Description

technical field [0001] The invention relates to a fungicide composition, in particular to a fungicide formula containing Bacillus subtilis and kresoxim-methyl, and a preparation method of the fungicide. Background technique [0002] Bacillus subtilis, English name (Bacillus subtilis), is a kind of Bacillus, Gram-positive bacteria, the surface of the colony is rough and opaque, dirty white or yellowish. Studies have confirmed that Bacillus subtilis can produce subtilisin, polymyxin, nystatin, gramicidin and other antibacterial substances with different structures and functions. It has obvious inhibitory effect on germs and has a wide antibacterial spectrum, which can prevent and control various plant diseases. Bacillus subtilis also has the following advantages: it has strong competition and colonization ability, seizes the living space of pathogenic bacteria, improves the soil microbial environment, prevents and interferes with the infection of pathogenic bacteria on plant ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/00A01N25/04A01P3/00A01N37/50
CPCA01N63/00A01N25/04A01N37/50
Inventor 吴淑科任承才杜荣锴
Owner 海南博士威生物科技有限公司
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