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Lactobacillus.crispatus for preventing female urogenital tract infection

A kind of Lactobacillus Campylobacter, bacteria technology, applied in the field of Lactobacillus Campylobacter prevention and treatment of women's genitourinary tract infection, can solve problems such as poor effect, toxic and side effects, etc.

Active Publication Date: 2016-02-24
INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the poor effect of antibiotics on genitourinary tract infections and the side effects of liver and kidney damage caused by antibiotics, efforts have been made to explore microecological therapies based on probiotic Lactobacillus preparations at home and abroad.

Method used

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  • Lactobacillus.crispatus for preventing female urogenital tract infection
  • Lactobacillus.crispatus for preventing female urogenital tract infection
  • Lactobacillus.crispatus for preventing female urogenital tract infection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Embodiment 1, isolation and identification of Lactobacillus flexus strain

[0032] 1. Isolation of Lactobacillus flexus strains

[0033] MRS medium: peptone 10.0g; beef extract 10.0g; yeast extract 5.0g; diammonium hydrogen citrate 2.0g; glucose 20.0g; Tween 80, 1.0mL; sodium acetate (CH 3 COONa·3H 2 O) 5.0g; Dipotassium hydrogen phosphate (K 2 HPO4·3H 2 O) 2.0g; Magnesium sulfate (MgSO 4 ·7H 2 O) 0.58g; manganese sulfate (MnSO 4 ·H 2 O) 0.25g; distilled water 1000mL; adjust to pH6.2~6.6.

[0034] More than 200 samples of vaginal secretions from healthy women without symptoms and signs of vaginitis were collected. The samples were numbered sequentially, inoculated in MRS liquid medium, and incubated anaerobically at 37°C for 24 hours. For each sample, 5 single colonies were randomly selected and cultured in 2 mL MRS liquid medium for 24 hours, and the colonies were numbered sequentially. Bacteria are amplified for bacterial conservation and DNA preparation. Hu...

Embodiment 2

[0045] Example 2, Functional analysis of Lactobacillus crispatus (Lactobacillus.crispatus) IMC-25

[0046] 1. Detection of antibacterial ability of Lactobacillus.crispatus IMC-25

[0047] The IMC-25 lactobacilli that were activated overnight were inoculated into 10mL MRS liquid medium at a ratio of 10% and cultured at 37°C for 16h, then cultured on a shaker at 37°C and 220r / min for 3h, and the fermentation broth was filtered and sterilized Store at 4°C for later use.

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Abstract

The invention discloses lactobacillus.crispatus for preventing female urogenital tract infection. The lactobacillus.crispatus specifically refers to lactobacillus.crispatus IMC-25. The lactobacillus.crispatus IMC-25 has higher anti-bacterial capacity, can effectively inhibit growth of extraneous pathogenic bacteria and other conditioned pathogens, also has higher adhesive function on vaginal epithelial cells and guarantees colonization of strains on vaginal epitheliums. The lactobacillus.crispatus IMC-25 has certain application prospect in the aspect of prevention of urogenital tract infection.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a lactobacillus flexus and its application, in particular to a lactobacillus flexus used for preventing and treating women's urogenital tract infection. Background technique [0002] Urogenital tract infection is a common disease that seriously affects the physical and mental health of women. Most of them are Escherichia coli or Staphylococcus aureus infection, and the prevalence rate is as high as 40%. Its treatment mainly relies on antibiotics, but the cure rate is low, and the recurrence rate can reach 40%. %above. [0003] In order to reduce the recurrence rate of genitourinary tract infection, new treatment options are urgently needed clinically. Due to the ineffectiveness of antibiotics in the treatment of urogenital tract infections and the side effects of liver and kidney damage caused by antibiotics, efforts have been made at home and abroad to explore microecological therapi...

Claims

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Application Information

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IPC IPC(8): C12N1/20A61K35/74A61P31/04C12R1/225
CPCA61K35/747C12N1/205C12R2001/225
Inventor 朱宝利张瑞芬律娜王黎明李晶
Owner INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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