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Oridonin-nano-selenium compound modified by GE11 as well as preparation method and application of oridonin-nano-selenium compound

A technology of oridonin and GE11, which is applied in the field of oridonin-nano-selenium complex and its preparation, can solve the problems of oridonin-nano-selenium complex without patents and literature reports, and achieve Good EGFR targeting function, inhibition of proliferation, and good stability

Inactive Publication Date: 2016-03-02
MACAU UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] At present, there have been related reports on melatonin nano-selenium, glucosidan nano-selenium, chitin nano-selenium, and liquid-phase polysaccharide nano-selenium in the prior art, but Oridonin A modified by GE11 polypeptide- Nano-selenium complex has no patents and literature reports so far

Method used

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  • Oridonin-nano-selenium compound modified by GE11 as well as preparation method and application of oridonin-nano-selenium compound
  • Oridonin-nano-selenium compound modified by GE11 as well as preparation method and application of oridonin-nano-selenium compound
  • Oridonin-nano-selenium compound modified by GE11 as well as preparation method and application of oridonin-nano-selenium compound

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] In this embodiment, a method for preparing a GE11 polypeptide-modified oridonin-nano-selenium complex comprises the following steps:

[0043] (1) sodium selenite is dissolved in water, is mixed with the sodium selenite solution of 50mM;

[0044] (2) Dissolving Rubescensin A in methanol to prepare 50 mM Rubescensin A solution;

[0045] (3) ascorbic acid is dissolved in water, is mixed with the ascorbic acid solution of 50mM;

[0046] (4) Chitosan is dissolved in the acetic acid aqueous solution of 0.1-1% by volume fraction, is mixed with the chitosan solution that concentration is 0.5wt%;

[0047] (5) Dissolving the GE11 polypeptide in water to prepare a 50 mg / mL GE11 polypeptide solution;

[0048] (6) Dissolving 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC) in water to prepare a 500 mM EDC solution;

[0049] (7) Mix 200 μL of sodium selenite solution with 100 μL of oridonin A solution and 600 μL of chitosan solution, then add 1600 μL of ascorbic a...

Embodiment 2

[0053] In this embodiment, a method for preparing a GE11 polypeptide-modified oridonin-nano-selenium complex comprises the following steps:

[0054] (1) sodium selenite is dissolved in water, is mixed with the sodium selenite solution of 40mM;

[0055] (2) Dissolving Rubescensin A in methanol to prepare 40 mM Rubescensin A solution;

[0056] (3) ascorbic acid is dissolved in water, is mixed with the ascorbic acid solution of 40mM;

[0057] (4) Chitosan is dissolved in the acetic acid aqueous solution of 0.1-1% by volume fraction, is mixed with the chitosan solution that concentration is 20wt%;

[0058] (5) Dissolving the GE11 polypeptide in water to prepare a 40 mg / mL GE11 polypeptide solution;

[0059] (6) Dissolving 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC) in water to prepare a 400mM EDC solution;

[0060] (7) Mix 25 μL of sodium selenite solution with 25 μL of oridonin A solution and 100 μL of chitosan solution, then add 250 μL of ascorbic acid s...

Embodiment 3

[0063] In this embodiment, a method for preparing a GE11 polypeptide-modified oridonin-nano-selenium complex comprises the following steps:

[0064] (1) sodium selenite is dissolved in water, is mixed with the sodium selenite solution of 60mM;

[0065] (2) Dissolving Rubescensin A in methanol to prepare 60 mM Rubescensin A solution;

[0066] (3) ascorbic acid is dissolved in water, is mixed with the ascorbic acid solution of 60mM;

[0067] (4) Chitosan is dissolved in the acetic acid aqueous solution of 0.1-1% by volume fraction, is mixed with the chitosan solution that concentration is 20wt%;

[0068] (5) Dissolving the GE11 polypeptide in water to prepare a 60 mg / mL GE11 polypeptide solution;

[0069] (6) Dissolving 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC) in water to prepare a 600mM EDC solution;

[0070] (7) Mix 1667 μL of sodium selenite solution with 1667 μL of oridonin A solution and 5000 μL of chitosan solution, then add 3333 μL of ascorbic ...

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Abstract

The invention relates to oridonin-nano-selenium compound modified by GE11 as well as a preparation method and an application of the oridonin-nano-selenium compound. The preparation method of the oridonin-nano-selenium compound comprises the following steps: (1) preparing a selenium compound, oridonin, a reducing agent and a stabilizer into solutions respectively, mixing the solutions, carrying out reaction at 0-37 DEG C for 5-20 h, and thus obtaining oridonin-nano-selenium solution; and (2) preparing GE11 polypeptide and a carboxyl activating agent into solutions respectively, adding the solutions into the oridonin-nano-selenium solution, and carrying out reaction at 0-37 DEG C for 5-20 h, and thus obtaining the oridonin-nano-selenium compound solution modified by the GE11 polypeptide. The oridonin-nano-selenium compound modified by GE11 is uniform in particle size distribution, good in water solubility, and good in stability. The pharmacological research proves that the oridonin-nano-selenium compound modified by GE11 has good EGFR targeting function, and the proliferation of cancer cells and the vascular endothelial cells can be effectively inhibited.

Description

technical field [0001] The invention relates to the field of nutrition, healthcare and medical treatment, in particular to a GE11 polypeptide-modified oridonin-nano selenium complex and a preparation method and application thereof. Background technique [0002] Dongling grass belongs to the Lamiaceae Fragrant Tea Vegetables genus, and its scientific name is also called Sumiya. It is mainly produced in Jiyuan County, Xinyang County, Henan Province, Taihang Mountain Hebi District, Shanxi Province, Taibai Mountain District, Shaanxi Province, Nanchuan County, Sichuan Province, Shibing County, Guizhou Province, and Xishui County. And Fenggang County in Zunyi Prefecture and other areas. Donglingcao tastes bitter, sweet, and slightly cold. It has the effects of clearing heat, relieving the exterior, reducing inflammation and relieving pain. Therefore, it can be used for the treatment of sore throat, tonsillitis, snake bites and rheumatic bone pain. At the same time, Rubescens japo...

Claims

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Application Information

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IPC IPC(8): A61K47/48A61K47/04A61K33/04A61K31/352A61P35/00
Inventor 皮江蔡继业金花江金环杨芬
Owner MACAU UNIV OF SCI & TECH
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