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Co-production even-molecular-weight-distribution fibroin-polypeptide and sericin-polypeptide powder and preparing method thereof

A molecular weight distribution, sericin polypeptide technology, applied in the preparation methods of peptides, chemical instruments and methods, peptides, etc., to achieve the effects of shortening soaking time, obvious water saving, and uniform molecular weight distribution

Inactive Publication Date: 2016-03-09
湖州新天丝生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a co-production of silk fibroin polypeptide and sericin polypeptide with uniform molecular weight distribution and its preparation method, which comprises cleaning, steam swelling, high-pressure degumming, secondary enzymatic hydrolysis, ultrafiltration, membrane separation and concentration, drying, etc. The composition of the process has the characteristics of water saving, energy saving, silk fibroin polypeptide, sericin polypeptide co-production, etc., which solves the shortcomings of the existing process

Method used

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Effect test

Embodiment 1

[0029] A method for co-producing silk fibroin polypeptides with uniform molecular weight distribution and sericin polypeptides, comprising the steps of:

[0030] 1. Cleaning and degumming of silk:

[0031] ① Weigh 100 kilograms of silk, soak the silk with tap water for 3 hours, then rinse it with pure water countercurrently for 3 times, and dry it with a centrifuge;

[0032] ②Wash the silk and swell with wet steam for 1.5 hours

[0033] ③According to the bath ratio (weight ratio) of silk: water = 1:6, add 100kg silk and 600kg pure water into the high-pressure degumming kettle, heat the high-temperature steam to boiling, keep the steam pressure of 0.15mPa, release the liquid for 1 hour, and use silk: Boil pure water = 1:5, wash 5 times, combine sericin solution and cleaning solution, take out silk fibroin, weigh 72kg dry.

[0034] 2. Enzymatic hydrolysis of silk protein:

[0035] ④ Put the combined sericin solution into the enzymatic hydrolysis kettle and heat it to 45°C, ad...

Embodiment 2

[0044] A method for co-producing silk fibroin polypeptides with uniform molecular weight distribution and sericin polypeptides, comprising the steps of:

[0045] 1. Cleaning and degumming of silk:

[0046] ① Weigh 100 kilograms of silk, soak the silk with tap water for 4 hours, then rinse it with pure water countercurrently for 3 times, and dry it with a centrifuge;

[0047] ②Wash the silk and swell with wet steam for 1 hour

[0048] ③According to the bath ratio (weight ratio) of silk: water = 1:6, add 100kg silk and 600kg pure water into the high-pressure degumming kettle, heat the high-temperature steam to boiling, keep the steam pressure of 0.15mPa, release the liquid for 1 hour, and use silk: Boil pure water = 1:5, wash 5 times, combine sericin solution and cleaning solution, take out silk fibroin, weigh 71.5kg dry.

[0049] 2. Enzymatic hydrolysis of silk protein:

[0050] ④ Put the combined sericin solution into the enzymatic hydrolysis kettle and heat it to 45°C, adj...

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Abstract

The invention discloses a co-production even-molecular-weight-distribution fibroin-polypeptide and sericin-polypeptide powder and a preparing method thereof. The method includes the working procedures of cleaning, steam swelling, high-pressure degumming, secondary enzymolysis, ultra-filtration, membrane separation, concentration, drying and the like. The method has the advantages that molecular weight distribution is even, water and energy are saved, and co-production of fibroin polypeptide and sericin polypeptide is achieved; the defects of existing technologies are well overcome. The silk polypeptide powder comprises the fibroin polypeptide and the sericin polypeptide, and has the advantages of being high in purity and bioactivity, moderate in molecular weight, white, free of peculiar smells and the like. The powder can be used for preparing biological materials, medical materials, health food and the like, and the pure natural aim, the high activity aim, the excellent functionality aim, the rich nutrient aim and the like can be achieved.

Description

technical field [0001] The invention relates to a method for co-producing silk fibroin polypeptide with uniform molecular weight distribution and sericin polypeptide Background technique [0002] Silk protein includes silk fibroin and sericin, which are rich in 18 kinds of amino acids, among which glycine, alanine and serine in silk fibroin account for more than 70%, and polar side group amino acids in sericin account for more than 73%. It is a natural nutritional substance with outstanding functions, which is widely used in biology, medicine, food, cosmetics, electronic materials, leather and fabrics, etc. [0003] At present, domestic silk reeling, silk weaving and other enterprises produce a large amount of silk waste every year. Due to many shortcomings in the existing preparation process, there are certain problems in the molecular weight distribution and quality of silk protein products, which affects the expansion of application fields, and the process water, energy ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/00C07K1/34
CPCC12P21/00Y02P20/10
Inventor 徐国文郭明方张敏彦刘玉梅
Owner 湖州新天丝生物技术有限公司
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