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Tissue culture and rapid propagation method for macadimia nuts

A tissue culture and nut technology, which is applied in the field of tissue culture and rapid propagation of macadamia nuts, can solve problems such as difficulty in rooting explants, and achieve the effects of shortening the time of tissue culture, improving the proliferation ability, and improving the quality of varieties.

Active Publication Date: 2016-03-16
广西合山市润鸿农业技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] Still another object of the present invention is to provide a liquid medium, which is atomized by the cultivation bed of the present invention, absorbs moisture and nutrients in the liquid medium through the 2 layers of flax fiber cloth at the bottom of the solid medium, thereby inducing rooting, And increase the rooting rate, thereby solving the problem of difficult rooting of macadamia nut explants

Method used

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  • Tissue culture and rapid propagation method for macadimia nuts

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Embodiment 1

[0034] The present invention provides a tissue culture rapid propagation method of macadamia nuts, comprising:

[0035] Step A, intercepting 4 cm long stem segments containing buds from adult macadamia trees as explants, each stem segment contains an axillary bud, and the explants are sterilized;

[0036] Step B, insert the explants obtained in step A into the induction medium and carry out induction culture for 20 days, the culture temperature is 23°C, the light intensity is 2000Lux, and the light is 13h every day. The formula of the induction medium is: 1 / 2MS, 6-benzyl adenine 0.2mg / L, gibberellin 0.2mg / L, vitamin B 1 0.45mg / L, agar 7g / L and sucrose 25g / L;

[0037] Step C, cutting the new long axillary bud seedlings cultivated in step B into stem segments with only one axillary bud under aseptic conditions, inserting into the rooting medium and cultivating them at a culture temperature of 25°C, including three stages in turn : In the first stage, the light intensity is 22...

Embodiment 2

[0050] A tissue culture rapid propagation method of macadamia nuts, comprising:

[0051] Step A, intercepting 4 cm long stem segments containing buds from adult macadamia trees as explants, each stem segment contains an axillary bud, and the explants are sterilized;

[0052] Step B, insert the explants obtained in step A into the induction medium for induction culture for 20-24 days, the culture temperature is 24°C, the light intensity is 2100Lux, and the light is 14h every day. The formula of the induction medium is: 1 / 2MS, 6-benzyl adenine 0.3mg / L, gibberellin 0.25mg / L, vitamin B 1 0.50mg / L, agar 7g / L and sucrose 25g / L;

[0053] Step C, cutting the new long axillary bud seedlings cultivated in step B into stem segments with only one axillary bud under aseptic conditions, inserting into the rooting medium and cultivating them at a culture temperature of 26°C, including three stages in turn : In the first stage, the light intensity is 2300Lux, and the light is 15h per day fo...

Embodiment 3

[0065] A tissue culture rapid propagation method of macadamia nuts, comprising:

[0066] Step A, intercepting 4 cm long stem segments containing buds from adult macadamia trees as explants, each stem segment contains an axillary bud, and the explants are sterilized;

[0067] Step B, insert the explants obtained in step A into the induction medium for induction culture for 24 days, the culture temperature is 25°C, the light intensity is 2200Lux, and the light is 15h every day. The formula of the induction medium is: 1 / 2MS, 6-benzyl adenine 0.4mg / L, gibberellin 0.3mg / L, vitamin B 1 0.55mg / L, agar 7g / L and sucrose 25g / L;

[0068] Step C, cutting the new long axillary bud seedlings cultivated in step B into stem segments with only one axillary bud under aseptic conditions, inserting into the rooting medium and cultivating them at a culture temperature of 27°C, including three stages in turn : In the first stage, the light intensity is 2400Lux, and the light is 16h per day for 1...

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Abstract

The invention discloses a tissue culture and rapid propagation method for macadimia nuts. The tissue culture and rapid propagation method for the macadimia nuts comprises the steps that stem sections with buds and with the length of 4 cm are cut out of mature macadimia nut trees to serve as explants, and the explants are subjected to disinfection treatment; the obtained explants undergo induced culture for 20-24 days, wherein the temperature is 23-25 DEG C, the light intensity is 2000-2200 Lux, and the lighting time is 13-15 hours every day; cultivated newly-grown axillary bud seedlings are cut into the stem sections under the aseptic conditions, and each stem section is only with one axillary bud and is planted in a rooting medium to be cultivated at the temperature of 25-27 DEG C. The tissue culture and rapid propagation method for the macadimia nuts sequentially comprises three stages: at the first stage, cultivation is performed for 12-14 days under the conditions of the light intensity of 2200-2400 Lux and the lighting time of 14-16 hours every day; at the second stage, cultivation is performed under the dark condition for 8-10 days; at the third stage, cultivation is performed under the conditions of the light intensity of 2800-3000 Lux and the lighting time of 18 hours every day till rooting is achieved. The problems of difficult tissue culture and rooting of the macadimia nuts and low rooting rate are solved by means of the tissue culture and rapid propagation method.

Description

technical field [0001] The invention relates to the field of tissue culture of macadamia nuts. More specifically, the present invention relates to a tissue culture rapid propagation method of macadamia nuts. Background technique [0002] Macadamia nuts, also known as Queensland chestnuts and macadamia nuts. Its kernels are rich in unsaturated fatty acids, proteins, vitamins, etc., with high nutritional value and unique flavor, known as the "king of dried fruits". The market prospect of macadamia nuts is promising, with good ecological and economic benefits. It is an edible nut with high oil content, rich nutrition and unique flavor. It has been in short supply in the international market. [0003] Macadamia nut seedling raising methods include seed seedling raising, cutting seedling raising, grafting seedling raising and plant tissue culture seedling raising method. Due to the poor genetic stability of macadamia seed breeding, seedlings are rarely propagated by single see...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 凌华彪
Owner 广西合山市润鸿农业技术有限公司
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