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Use Of Ginsenoside F2 For Prophylaxis And Treatment Of Liver Disease

A technology of ginsenosides and uses, applied in the field of liver disease prevention or treatment of ginsenoside F2, which can solve the problems that the therapeutic effect of ginsenoside F2 on liver diseases has not been revealed

Active Publication Date: 2016-03-16
KOREA ADVANCED INST OF SCI & TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the liver disease therapeutic effect of ginsenoside F2 has not been revealed

Method used

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  • Use Of Ginsenoside F2 For Prophylaxis And Treatment Of Liver Disease
  • Use Of Ginsenoside F2 For Prophylaxis And Treatment Of Liver Disease
  • Use Of Ginsenoside F2 For Prophylaxis And Treatment Of Liver Disease

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0074] Embodiment 1. The effect of alleviating the liver damage caused by alcohol and inhibiting the formation of fatty liver

[0075] The 8-week-old male rat (weight is 25~28g) is fed normally for two weeks, and at the same time, the ginsenoside F2 (expressed as GF2 in the accompanying drawing) of 50mg / kg is dissolved in the ethanol of 3g / kg and in the afternoon every day It was orally administered to male mice at about 3-4 o'clock, and the male mice were killed two weeks later to perform the following experiments.

[0076] The preparation method of ginsenoside F2 is as follows. Put the leaves and roots of other ginseng including Korean ginseng, American ginseng, and bamboo ginseng into 20 times the volume of 80% alcohol, extract twice or more, and dry to obtain crude saponins. The crude saponin was dissolved in water again, and after being adsorbed to HP-20 resin, 100% water was used to wash to remove sugar, and then 40% alcohol was used to wash once to remove preferentiall...

Embodiment 2

[0091] Example 2. Experiments on co-cultured hepatocytes and hepatic stellate cells

[0092] (1) Inhibition of fat synthesis in hepatocytes and hepatic stellate cells

[0093] Such as Figure 5a In the schematic diagram shown, after isolating mouse hepatocytes and hepatic stellate cells, hepatic stellate cells (HSCs) were placed on the bottom surface of the lower side and hepatocytes were placed on the upper side, and co-culture was carried out. When culturing, 100 mM ethanol was treated for 12 hours, and then 30 μM ginsenoside F2 was treated for 6 hours or 12 hours.

[0094] Figure 5b The results of Western blotting were performed on co-cultured hepatocytes recovered. Unlike the hepatocytes treated with ethanol alone, the expression of fat-reducing pAMPK was increased, and conversely, the expression of fat-synthesizing SREBP1c and FAS was decreased in the case of simultaneous treatment with ginsenoside F2.

[0095] Figure 5c It is the result of real-time PCR of co-cult...

Embodiment 3

[0101] Example 3. Inhibitory effect of liver inflammatory changes by increasing regulatory T cells

[0102] Rats were fed normally for two weeks, and at the same time, 3 g / kg was administered to each individual for two weeks. At this time, the vehicle was administered to the first group (E+Veh), and ginsenoside F2 was administered to the second group (E+GF2).

[0103] Thereafter, mononuclear cells were isolated from the liver, and stained with macrophage markers CD11b and F4 / 80 for flow cytometry analysis. As a result, it was confirmed that the proportion of macrophages decreased when treated with ginsenoside F2 ( Figure 6a ), flow cytometry analysis was carried out by staining with markers CD11b and Gr-1 of neutrophils, and it was confirmed that when ginsenoside F2 was used for treatment, the proportion of neutrophils decreased ( Figure 6b ). Macrophages and neutrophils are cells closely related to inflammatory response, so it can be seen that ginsenoside F2 can relieve i...

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Abstract

The inventoin provides a use of ginsenoside F2 in the prevention, improvement or treatment of liver disease, and a pharmaceutical composition, a health functional food, and a feed composition including ginsenoside F2. Ginsenoside F2 inhibits fat synthesis and accumulation in the liver, and increases distribution of regulatory T cells capable of inhibiting activity of inflammatory cells, thereby preventing hepatitis, and also increases expression of anti-inflammatory cytokine IL-10 in regulatory T cells, and inhibits differentiation of naive T cells into Th17 cells, and is thereby effectively used for the treatment of various liver diseases.

Description

technical field [0001] The invention relates to a ginsenoside F2 pharmaceutical composition for preventing, improving or treating liver disease, a health functional food and a feed composition. Background technique [0002] Alcoholic liver disease can be roughly divided into alcoholic fatty liver, alcoholic hepatitis, and alcoholic cirrhosis based on clinical symptoms. Although there are individual differences depending on genetic characteristics and gender, it is known that if you consume more than 80g of alcohol per day, The possibility of inducing liver disease is higher. [0003] In particular, alcoholic fatty liver is a disease in which fatty acid is excessively produced by the metabolism of alcohol and fat accumulates in the liver. When the proportion of lipid accumulated in the liver is 5% or more, it is defined as fatty liver. Most of the accumulated lipids are neutral fats (triglyceride (triglyceride)). [0004] There are many causes of alcoholic fatty liver. Fir...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/704A61P1/16A23L33/11A23K20/163
CPCA61K31/704A23L33/10A61P1/16A61P3/06A61P29/00A61P35/00
Inventor 郑元日郑朱延金善昌林完泽
Owner KOREA ADVANCED INST OF SCI & TECH
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