Composite nanodot based on carbon nanodot and preparation technology thereof

A technology of carbon nano-dots and preparation process, which is applied in preparations for in vivo experiments, luminescent/biological dyeing preparations, drug delivery, etc., can solve problems such as poor stability, high sex-to-noise ratio, short metabolic cycle, etc., and achieve stability Good, good monodispersity, low toxicity effect

Inactive Publication Date: 2016-03-16
WENZHOU INST OF BIOMATERIALS & ENG
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] In recent years, near-infrared fluorescent probes have become a research hotspot because of their large sex-to-noise ratio, strong background interference, and strong biological penetration (can image deeper tissues). The fluorescent imaging dye certified by the U.S. Food and Drug Administration (FDA) has an emission wavelength of about 700 nanometers and belongs to the near-infrared region. However, due to the poor stability of this dye in vivo and the short metabolic cycle, it is necessary to construct a carrier for loading dye molecules. To improve its stability in the biological environment

Method used

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  • Composite nanodot based on carbon nanodot and preparation technology thereof
  • Composite nanodot based on carbon nanodot and preparation technology thereof
  • Composite nanodot based on carbon nanodot and preparation technology thereof

Examples

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Effect test

Embodiment 1

[0033] Example 1: Take 2 milliliters of Lycium barbarum extract and 2 milliliters of methylene blue solution (0.5 mg / ml) and mix them, and dilute it with ultra-pure water 1 time to prepare a precursor solution, put the precursor solution into a 5 milliliter microwave reactor special glass bottle, And it was placed in a microwave reactor, and the reaction conditions were set at 180 degrees Celsius for 30 minutes. The reaction system was left to stand for 50 minutes, centrifuged, and the supernatant liquid was retained, and the composite carbon nano-dots were obtained after ultrafiltration. The concentration of methylene blue in the composite nano-dots measured by the ultraviolet spectrophotometer standard curve method is 5.6 micrograms per milliliter; measured by the laser particle size analyzer based on the principle of dynamic light scattering, the particle size distribution of the composite carbon nano-dots is 179 ± 77.1 nanometers (see attached Figure 2a ), the surface pot...

Embodiment 2

[0034] Example 2: Mix 10 ml of freshly ground soybean milk with 1 ml of methylene blue solution (0.5 mg / ml) and dilute it 10 times with ultrapure water to prepare a precursor solution. Take 5 ml of the precursor solution and put it into a 5 ml microwave reactor glass bottle, and it was placed in a microwave reactor, and the reaction conditions were set at 100 degrees Celsius for 5 hours. The reaction system was left to stand for 60 minutes, then centrifuged, and the supernatant liquid was retained, and the composite carbon nano-dots were obtained after ultrafiltration. The concentration of methylene blue in the composite carbon nano-dots was measured to be 8.6 μg / ml by the UV spectrophotometer standard curve method. According to the principle of dynamic light scattering and measured by laser particle size analyzer, the particle size distribution of the composite carbon nano-dots supported by methylene blue is 197.9±79.7 nm (see attached Figure 3a ), the surface potential is ...

Embodiment 3

[0035] Example 3: Mix 1 ml of edible milk with 10 ml of methylene blue solution (0.5 mg / ml) and dilute it 5 times with ultrapure water to prepare a precursor solution, take 5 ml of the precursor solution and put it into 5 ml of special glass for microwave reactor bottle, and it was placed in a microwave reactor, and the reaction conditions were set at 160 degrees Celsius for 2 hours. The reaction system was left to stand for 1 hour, and the supernatant was retained by centrifugation, and the composite carbon nano-dots were obtained after dialysis treatment. The concentration of methylene blue in the composite carbon nano-dots measured by the standard curve method was 1.8 μg / ml. According to the principle of dynamic light scattering and measured by a laser particle size analyzer, the particle size of the composite carbon nano-dots loaded with methylene blue is 434.4±196.8 nanometers (see attached Figure 4a ), the surface potential is 4.92±2.88 mV (see attached Figure 4b); t...

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Abstract

The invention discloses a preparation technology of a composite nanodot based on a carbon nanodot, and application of the composite nanodot in the field of fluorescence imaging. According to the structure of the composite nanodot, a main component is the carbon nanodot which is the material with the quite good biocompatibility, a load component is methylthionine chloride, the particle diameter ranges from 100 nanometers to 500 nanometers, and the potential ranges from -30 mV to 10 mV. The preparation technology is safe, fast, easy and convenient to operate, the cost is low, industrial production is easy to achieve, and the technology is general for preparing the composite carbon nanodot. On the basis that the composite carbon nanopot has the good biocompatibility and safety and is high in fluorescence imaging sensitivity, the composite carbon nanopot is expected to be more widely applied in the fields of biomedical imaging, targeting diagnosis and therapy, medicine screening and optimization, in-vivo marking and tracking and the like, and has the potential value in the aspects of individual medical treatment and the like.

Description

technical field [0001] The invention relates to a composite carbon nano-dot applied to fluorescent bioimaging technology and its preparation and purification process. Background technique [0002] Fluorescence imaging is an emerging in vivo and ex vivo bio-optical imaging technology in the past ten years. It has become an in vitro biological sample due to its many advantages such as easy operation, little damage to organisms, intuitive imaging results, fast measurement, high sensitivity and low measurement cost. , An ideal method for imaging small living animals. Using this imaging technology, the distribution of marked genes or cells in living animals and the participating biological and pathological processes and responses can be directly observed in real time, so it is widely used to study the occurrence and development of various diseases, the mechanism of action of drugs, In vivo metabolism and screening of new drugs. Fluorescent probe is the core component of fluores...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K49/00
CPCA61K49/005A61K49/0032A61K49/0093
Inventor 刘哲徐齐恩汪婕宋远会李宜鸿
Owner WENZHOU INST OF BIOMATERIALS & ENG
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