A kind of protein and sugar grafting reaction equipment and preparation method of glycosylated protein

A technology of grafting reaction and protein, applied in the field of protein deep processing, can solve the problems of low production efficiency, discontinuous reaction, long grafting reaction time of protein and sugar, etc. Effect

Active Publication Date: 2017-08-08
SHENYANG NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In order to solve the above technical problems, the present invention provides a protein and sugar grafting reaction equipment and a preparation method for glycosylated protein, which improves the degree of grafting of protein and sugar grafting reaction, and improves the glycosylated protein. Emulsification and solubility solve the problems of long reaction time, low production efficiency and discontinuous reaction of protein and sugar grafting

Method used

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  • A kind of protein and sugar grafting reaction equipment and preparation method of glycosylated protein
  • A kind of protein and sugar grafting reaction equipment and preparation method of glycosylated protein
  • A kind of protein and sugar grafting reaction equipment and preparation method of glycosylated protein

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] 1) Set the amount of rice protein required for the grafting reaction on the feed control display to be 10g, and the amount of distilled water to be set to 1000mL;

[0047] 2) Set the pH required for preparing the protein solution on the pH control display to 12, and set the stirring speed required for preparing the protein solution on the stirring control display to 300r / min;

[0048] 3) Start the grafting reaction equipment, the reaction time is 30min, and suspend the equipment after making protein solution;

[0049] 4) The amount of sugar required for the grafting reaction is set to 30g on the feed control display, and the connection is set on the pH control display, temperature control display, microwave power control display, pressure control display, stirring control display and discharge control display. The pH required for the branching reaction is 10.5, the temperature is 110°C, the microwave power is 100W, the pressure is 100MPa, the stirring speed is 300r / min,...

Embodiment 2

[0052] The difference from Example 1 is:

[0053] In step 1), the amount of rice protein added is set to 20g, the amount of distilled water is set to 1500mL, and the stirring speed of the protein liquid is 400r / min;

[0054] The sugar added in step 4) is maltodextrin, the added amount is set to 60g, the reaction temperature is 100°C, the microwave power is 300W, the pressure is 80MPa, the stirring speed is 400r / min, and the reaction time is 15min.

Embodiment 3

[0056] The difference from Example 1 is:

[0057] In step 1), the amount of rice protein added is set to 10g, the amount of distilled water is set to 1500mL, and the stirring speed of the protein liquid is 500r / min;

[0058] The sugar added in step 4) is glucose, the amount added is 20g, the reaction temperature is 90°C, the microwave power is 500W, the pressure is 60MPa, the stirring speed is 500r / min, and the reaction time is 25min.

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Abstract

The invention belongs to the technical field of protein deep processing, and in particular relates to a protein and sugar grafting reaction equipment and a preparation method of glycosylated protein. When using this equipment for the grafting reaction of protein and sugar, the reaction substrate enters the reaction chamber from the feeding component, and the pH control component, temperature control component, pressure control component and stirring control component jointly set and adjust the reaction conditions. After the reaction is completed , the reaction product is discharged from the discharge assembly into the post-processing device, and the target glycosylated protein is obtained after processing. Using the equipment provided by the invention to carry out the grafting reaction improves the grafting degree of the protein and sugar grafting reaction, improves the emulsification and solubility of the protein after glycosylation, and solves the problem of long grafting reaction time between the protein and sugar. Low production efficiency and discontinuous response.

Description

technical field [0001] The invention belongs to the technical field of protein deep processing, and in particular relates to a protein and sugar grafting reaction equipment and a preparation method of glycosylated protein. Background technique [0002] Glycosylation modification is based on the carbonylamino reaction between the free amino group of the amino acid side chain in the protein molecule and the carbonyl group at the reducing end of the sugar molecule - Maillard reaction, which does not require any chemical reagents as a catalyst, and the reaction can be achieved only by heating Do it spontaneously. Mainly used in soy protein, rice protein, whey protein, casein, etc. Judging from the literature reported so far, there are mainly two methods for grafting proteins and sugars: dry heat method and wet heat method, but the reaction time of these two methods is longer, especially the dry reaction, and few Ten hours, as many as a few days or even weeks, is not conducive ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01J19/18B01J19/00B01J4/02C07K1/107
CPCB01J4/001B01J4/02B01J19/0006B01J19/0013B01J19/18B01J2204/002B01J2204/005B01J2219/00141B01J2219/00162B01J2219/00189C07K1/1077
Inventor 肖志刚许岩高育哲王鹏刘春景李雪武英华
Owner SHENYANG NORMAL UNIV
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