Preparation method for heparin sodium
A technology of heparin sodium and heparinase, which is applied in the biological field, can solve the problems of large investment in hardware facilities for the production of heparin sodium, high requirements for material and environmental temperature control, and large hardware investment. The effect of low environmental temperature requirements and small investment in hardware facilities
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Examples
Embodiment 1
[0020] Step 1: Stir and dissolve the crude sodium heparin raw material (commercially available raw material, potency 65-105IU / mg) with 1%-3% NaCl solution, the concentration of crude sodium heparin is 8%-16% (mass percentage concentration ), adjust the pH to 7.5-9.0 with 5M NaOH solution, heat up to 50-55°C, add heparin enzymolysis trypsin according to 0.1-0.3% of the mass of the crude product heparin sodium solution, keep warm for 4-5 hours, and use 5M NaOH Adjust the pH value of the solution to 9.0-11, heat up to 85°C-90°C, cool to room temperature, filter to remove the precipitate, and precipitate the filtrate with 0.8-1.5 times the volume of 95% ethanol for more than 4 hours, and collect the precipitate A;
[0021] Step 2: Stir and dissolve the precipitate A with pure water, the dissolved concentration is 10%-20% (mass percentage), adjust the pH to 8.5-11 with 5M NaOH solution, and press 0.1%-5% of the mass of the precipitate A aqueous solution Add protein flocculation and...
Embodiment 2
[0026] The heparin enzymolysis trypsin in step 1 is selected as compound trypsin containing trypsin;
[0027] The protein flocculation precipitant in step 2 is selected polyaluminum chloride for use;
[0028] The other steps were the same as in Example 1 to obtain the refined heparin sodium.
Embodiment 3
[0030] The heparin enzymolysis trypsin in step 1 is selected as neutral trypsin;
[0031] The protein flocculation precipitant in step 2 is selected sodium polyacrylate for use;
[0032] The other steps were the same as in Example 1 to obtain the refined heparin sodium.
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com