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Method for constructing sequencing library based on blood samples, and uses of method in determination of fetal genetic abnormality

A technology for blood samples and sequencing libraries, which is applied in the field of biomedicine and can solve problems that need to be improved.

Active Publication Date: 2016-03-16
北京六合华大基因科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, the current related technologies for nucleic acid sequencing still need to be improved

Method used

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  • Method for constructing sequencing library based on blood samples, and uses of method in determination of fetal genetic abnormality
  • Method for constructing sequencing library based on blood samples, and uses of method in determination of fetal genetic abnormality
  • Method for constructing sequencing library based on blood samples, and uses of method in determination of fetal genetic abnormality

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Experimental program
Comparison scheme
Effect test

Embodiment

[0123] Example: The detection of fetal chromosome triploid variation was performed on the blood plasma of 36 pregnant women.

[0124] 1. Sample collection and processing:

[0125] (1) Take 2ml of maternal peripheral blood during pregnancy, centrifuge at 1600g, 4°C for 10 minutes, separate blood cells and plasma, and then centrifuge the plasma at 16000g, 4°C for 10 minutes to further remove residual white blood cells. SnoMag for Maternal Plasma TM CirculatingDNAKit (SNOVA) was used to extract plasma DNA, and finally the DNA was dissolved in 50ulTE solution.

[0126] 2. Construction of sequencing library

[0127] 2.1 rSAP processing

[0128] Use the following reaction system for rSAP treatment for dephosphorylation

[0129] DNA50ul

[0130] rSAP(1U / ul)6ul

[0131] 10XNEBuffer26ul

[0132] After mixing, put it on the PCR machine for reaction, the program is: 37°C / 45min, 65°C / 10min, 0.1°C / s uniform cooling to 4°C.

[0133] 2.2 End Repair

[0134] The reaction is carried o...

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Abstract

The present invention provides a method for constructing a sequencing library based on blood samples, a nucleic acid sequencing method, a method for determining fetal genetic abnormality in a sample, an apparatus for constructing a sequencing library based on blood samples, a nucleic acid device, and a system for determining fetal genetic abnormality in a sample.

Description

technical field [0001] The present invention relates to the field of biomedicine, in particular, the present invention relates to a method for constructing a sequencing library based on a blood sample and its use in determining fetal genetic abnormalities. More specifically, the present invention relates to providing a method for constructing a sequencing library based on a blood sample, a nucleic acid sequencing method, a method for determining fetal genetic abnormalities in a sample, a device for constructing a sequencing library based on a blood sample, A nucleic acid sequencing device and a system for determining fetal genetic abnormalities in a sample. Background technique [0002] High-throughput sequencing has become one of the foundations of modern molecular biology, biotechnology, medicine and many other fields. In recent years, research on rapid, accurate, and economical methods for determining gene expression levels and nucleotide sequences has continued to innov...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12M1/34C12M1/00C40B50/06C40B60/14
Inventor 张艳艳陈芳蒋慧郭宇来曾鹏徐讯杨焕明丹尼斯·G·博林格弗拉迪米尔·I·巴什基洛夫海曼舒·塞西
Owner 北京六合华大基因科技有限公司
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