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A kind of preparation method of glyceride type pufa

A technology of glyceride type and glycerin, which is applied in the field of preparation of glyceride type PUFA, can solve the problems of low triglyceride content, low substrate conversion rate, and low enzyme reaction efficiency, so as to improve conversion rate and good economy and environmental protection, and the effect of improving catalytic efficiency

Active Publication Date: 2018-11-02
SOUTH CHINA UNIV OF TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the problems of low enzymatic reaction efficiency, low substrate conversion rate and low triglyceride content in glycerides in the existing preparation method of glyceride PUFA, the purpose of the present invention is to provide a rapid preparation method of glyceride PUFA

Method used

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  • A kind of preparation method of glyceride type pufa

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Add 10Kg of PUFA concentrate (from tuna oil, PUFA composition is ALA 1.6%, AA 9.8%, EPA7.8%, DPA 16.4%, DHA 50.8%) and glycerin 1Kg into the mixing tank, under the condition of magnetic stirring, such as figure 1 As shown, the reactant is pumped and flows through the packed bed enzyme reactor 5 (the temperature of the enzyme reaction is 50°C, and the contact time of the material with immobilized lipase Novozym 435 is 5min), and then heat treatment is carried out in the heat treatment device 7, at 100 stay in the state of ℃ for 3 minutes, then return to the material tank 1 after cooling down to 50 ℃ through the cooling device 8, the dosage of immobilized lipase Novozym435 is 100g, the reaction temperature is 50 ℃, and the whole reaction system is placed in a vacuum with a pressure of 100Pa state. Adjust the flow rate of the material to make it circulate in the system. After 12 hours, take a sample to analyze the composition of the reaction product. The conversion rate of...

Embodiment 2

[0026] Add glycerin 1Kg, CLA ethyl ester concentrate 9Kg (CLA content is 80.3%) in mixing tank, under the situation of magnetic stirring, as figure 1 As shown, the reactant is transported through the pump and flows through the enzyme reactor 5 (the temperature of the enzyme reaction is 50° C., and the contact time of the material with the immobilized lipase Novozym435 is 5 minutes), and then heat treatment is carried out in the heat treatment device 7, at 110° C. stay at 2.5min, then return to the material tank 1 after being cooled to 50°C by the cooling device 8, the amount of immobilized lipase Novozym435 is 100g, the reaction temperature is 50°C, and the whole reaction system is placed in a vacuum state with a pressure of 100Pa . Adjust the flow rate of the material to make it circulate in the system. After 12 hours, take a sample to analyze the composition of the reaction product. The conversion rate of CLA is 93.1%, the content of triglyceride is 68.4%, the content of dig...

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Abstract

The invention discloses a method for preparing glyceride type polyunsaturated fatty acids, which comprises steps: (1) acyl donors of PUFA and glycerinum make contact with immobilized lipase to react after being mixed, and the whole reaction is completed in a vacuum condition; (2) a materials after the reaction in the step (1) is thermally treated at least 1.5n min in 90-120 DEG C, n=(150-T) / 20, the T is the temperature of thermal treatment, the material is added into a mixed material in the step (1) to recycle the reaction of the step (1) and (2) after the material is cooled to the temperature of the reaction in the step (1); and (3) when the reaction basically achieves balance, reaction products are separated, an oil phase is recycled, and is purified at last to obtain a glyceride type PUFA product. The method for preparing the glyceride type polyunsaturated fatty acids improves reaction efficiency by controlling components of glyceride through thermal treatment, reduces reaction time, improves yield of triglycerides, and enables conversion ratio of the acyl donors of the PUFA to be above 90%.

Description

technical field [0001] The invention relates to a preparation method of glyceride type PUFA. Background technique [0002] Polyunsaturated fatty acids (PUFA) generally refer to straight-chain fatty acids containing two or more double bonds and a carbon chain length of 18 to 22 carbon atoms. Common PUFAs that have special physiological functions on the human body mainly include eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA), eicosapentaenoic acid (DPA), arachidonic acid (AA), Stearidonic acid (SDA), α-linolenic acid (ALA), γ-linolenic acid (GLA). Due to their unique physiological functions, PUFA has more and more applications in the fields of functional food, medicine, and health care products. These PUFAs are mostly derived from marine fish oil, algae oil or certain vegetable oils. However, the PUFA content in products from these sources is low, especially the PUFA content in marine fish oil and algae oil is generally not higher than 50%, and they also contain a ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/64
Inventor 谢应根王卫飞王永华严慧玲杨博马永钧周小敏
Owner SOUTH CHINA UNIV OF TECH