Aqueous two-phase system and application thereof to enrichment of trace amount of aromadendrene

An aqueous two-phase system, the technology of vanillene, which is applied in the technological fields of food, biotechnology and analytical chemistry, can solve the problems of low vanillene residue, large error of analysis method, interference of determination purity, etc. Pure, easy to handle, fast mass transfer and equilibration results

Inactive Publication Date: 2016-03-23
CHANGAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In view of the complex operation existing in the existing detection method of sweetnillene, the residual amount of sweetnillene in the sample is low, the detection is difficult, the error of the analysis method is large, the sample matrix and the extraction process have pure interference on the determination of sweetnillene, etc. Difficulty, the object of the present invention is to provide a new method for separating and enriching trace amounts of sweetmanillene in the food ingredient Zanthoxylum oleoresin of imidazole ionic liquid two-phase system

Method used

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  • Aqueous two-phase system and application thereof to enrichment of trace amount of aromadendrene
  • Aqueous two-phase system and application thereof to enrichment of trace amount of aromadendrene
  • Aqueous two-phase system and application thereof to enrichment of trace amount of aromadendrene

Examples

Experimental program
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Effect test

Embodiment 1

[0058] Take 5.8 of 1-ethyl-3-methylimidazole tetrafluoroborate ionic liquid, add 1.411g of acetone and an appropriate amount of distilled water to make the total mass of the system 10g. Then add 2 g of vanillaene to be enriched solution, shake in a constant temperature shaker at 40° C. and pH 2.0 for 30 min, and then stand for extraction for 2 h after phase separation.

[0059] The concentration of remaining vanillane in the organic phase was determined, and the extraction rate of vanillane was calculated from the content of vanillane in the organic phase before and after the extraction. The extraction rate of vanillane was 96%, indicating that this invention has a good effect on trace vanillane in Zanthoxylum oleoresin. Separation and enrichment.

Embodiment 2

[0061] The extraction method in this example is the same as that in Example 1, except that the conditions in the extraction process are different. In this example, the amount of ionic liquid 1-ethyl-3-methylimidazole tetrafluoroborate added is 6.339g, The remaining conditions remain unchanged.

[0062] The concentration of remaining vanillane in the organic phase was determined, and the extraction rate of vanillane was calculated from the content of vanillane in the organic phase before and after extraction to 92.4%, indicating that this invention has a general extraction effect on trace vanillene in Zanthoxylum oleoresin .

Embodiment 3

[0064] The extraction method in this example is the same as that in example 1, except that the conditions in the extraction process are different. In this example, the amount of ionic liquid 1-ethyl-3-methylimidazole tetrafluoroborate added is 5.335g. The remaining conditions remain unchanged.

[0065] The concentration of remaining vanillane in the organic phase was determined, and the extraction rate of vanillane was calculated from the content of vanillane in the organic phase before and after extraction to 91.3%, indicating that this invention has a general extraction effect on trace vanillane in Zanthoxylum oleoresin .

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Abstract

The invention discloses an aqueous two-phase system and application thereof to enrichment of trace amount of aromadendrene. The aqueous two-phase system is prepared from, by mass, 56.7-60.38% of 1-ethyl-3-methylimidazole tetrafluoroborate ion liquid, 13.02-15.48% of acetone and the balance water, wherein the mass ratio of the 1-ethyl-3-methylimidazole tetrafluoroborate ion liquid, the acetone and the water is 100%. The extraction rate of the aromadendrene of the aqueous two-phase system can reach 96%, and the aqueous two-phase system has the advantages that extract is pure, equipment is simple, operation is easy to conduct, and the detection and recovery rate of samples is high. Processing of seasoner of the type of the nation can be facilitated, meanwhile operation is simple, interference of alpha-caryophyllene impurity can be excluded, and the aqueous two-phase system is applicable to quantitative analysis of aromadendrene in xanthoxylum oleoresin.

Description

Technical field [0001] The invention belongs to the technical field of food and biotechnology and analytical chemistry, and specifically relates to a two-phase aqueous system and its application for enriching trace vanillene. Background technique [0002] Zanthoxylum bungeanum is a main spice seasoning. Its use form is whole grain and Zanthoxylum bungeanum powder, which affects the sensory quality of food. Zanthoxylum bungeanum is easy to oxidize and mold, and it is difficult to store. At the same time, it is difficult to measure during the food supply process. Seriously affect the quality of food in our country. At present, my country’s export of pickles, such as Fuling mustard tuber, has adopted Zanthoxylum oleoresin as the seasoning. The main component of Zanthoxylum oleoresin is vanillaene, which makes the oleoresin have a stronger fragrance and lifelike effect. Vanillane is a Sesquiterpene , Existing in the Eucalyptus family Essential oil ( Eucalyptus oil ), it can be us...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/34
Inventor 李宇亮邱斯思徐帅卢筱佳孙钰琨
Owner CHANGAN UNIV
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