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Establishing method of self-adaptive model for detection of content of protein of rapeseeds on basis of mid-infrared spectrum

A protein content and self-adaptive model technology, which is applied in the field of agricultural inspection, can solve the problems of inability to meet the grain size of small samples of rapeseed, and unable to measure the protein content of rapeseed quickly, accurately and non-destructively, and achieves that the real value of the background technology is close to, The effect of reducing the interference of invalid information of the sample and high sensitivity

Active Publication Date: 2016-03-30
INST OF SOIL SCI CHINESE ACAD OF SCI
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  • Description
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  • Application Information

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Problems solved by technology

However, the existing mid-infrared spectroscopy technology still has certain requirements on the demand for test samples, which cannot meet the requirements of small rapeseed samples with low grain content. , precise, non-destructive determination of

Method used

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  • Establishing method of self-adaptive model for detection of content of protein of rapeseeds on basis of mid-infrared spectrum
  • Establishing method of self-adaptive model for detection of content of protein of rapeseeds on basis of mid-infrared spectrum
  • Establishing method of self-adaptive model for detection of content of protein of rapeseeds on basis of mid-infrared spectrum

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Embodiment 1

[0031] Example 1, based on mid-infrared photoacoustic spectroscopy to establish a fast, accurate and non-destructive adaptive model of rapeseed protein content:

[0032] 180 rapeseed samples were tested, and the samples were randomly classified, of which 70% (126 samples) were used as training sample sets, and 30% (54 samples) were sample sets to be tested.

[0033] 1. Take 100-200 mg of each sample from the training sample set and the sample set to be tested, and place it in the sample pool of the Fourier transform mid-infrared spectrometer (Nicolet8700, ThermoFisher Scientific, USA)-photoacoustic accessory (PA300, MTEC, USA), Put the sample into the photoacoustic cell for no more than 2 / 3. After placing the sample, close the sample cell, open the high-purity helium valve, and purge with high-purity helium to remove water vapor and carbon dioxide gas in the photoacoustic cell. Collect mid-infrared photoacoustic spectral information, the collection wavelength range is 4000-800...

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Abstract

An establishing method of a self-adaptive model for detection of content of protein of rapeseeds on the basis of a mid-infrared spectrum comprises steps as follows: I, mid-infrared photoacoustic spectrum information is sampled and acquired, continuous scanning is performed for multiple times, and an average spectrum is obtained; II, the content of the protein of the rapeseeds is chemically analyzed, and a chemical reference value is obtained; III, noise elimination, smoothing and standardized preprocessing are performed on the spectrum information; IV, spectrum data are calculated with an Euclidean distance method, and sequencing from small to large is performed; V, a prediction model based on the spectrum information is established; VI, a mid-infrared photoacoustic spectrum of to-be-tested samples is taken into the model, the content of the protein of the to-be-tested rapeseed samples is calculated and predicted, and a predicted value is obtained. The method is high in sensitivity, simple to operate and nondestructive to samples, the content of required samples is small, no chemical reagent is used, the method has the universality, the prediction result is accurate, invalid information interference of the samples can be effectively reduced, a sample modeling set can be optimized and simplified, and model calculation is quick and accurate.

Description

technical field [0001] The invention belongs to the field of agricultural detection methods, and in particular relates to a method for establishing an adaptive model for rapid, accurate and nondestructive detection of rapeseed protein content based on mid-infrared spectroscopy. Background technique [0002] Rapeseed is one of the four major oil crops in the world. The perennial sown area of ​​rapeseed in China is 7 million hectares, with a total output of 12 million tons, accounting for more than 60% of the total output of edible vegetable oil in the country, far exceeding other major rapeseed producing countries in the world. Rapeseed production occupies an extremely important position in China's national economy and people's livelihood. The detection and monitoring of the nutritional quality of rapeseed is an important technical support for China's rapeseed production. Nitrogen content is an important quality index of rapeseed. The protein content in the rapeseed cake afte...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/3563
CPCG01N21/3563
Inventor 杜昌文马菲周健民
Owner INST OF SOIL SCI CHINESE ACAD OF SCI
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