Supercharge Your Innovation With Domain-Expert AI Agents!

A kind of polyphenol diglycoside compound and its preparation method and application

A technology of diglycoside and compound, applied in the field of polyphenol diglycoside compound and preparation thereof, can solve the problem of less research on polyphenol glycoside compound, and achieve the effects of high product purity, simple operation and high extraction yield

Inactive Publication Date: 2018-07-06
ZHEJIANG UNIV
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few studies on polyphenol glycosides. For this reason, the research on the active ingredients of natural polyphenol glycosides in the whole plant of Zhulan can provide new ideas for the utilization of the whole plant.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of polyphenol diglycoside compound and its preparation method and application
  • A kind of polyphenol diglycoside compound and its preparation method and application
  • A kind of polyphenol diglycoside compound and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1 Preparation of polyphenol diglycoside compound

[0026] Take 5kg of the whole plant of Chloranthus spicatus (Thunb.) Makino, dry it, crush it, soak it in methanol (40L) for 2 weeks, suspend the soaking solution in 1L distilled water after concentration (100mL), and add Butanol extraction, the n-butanol extract is concentrated to obtain the extract; use silica gel (100 mesh, 100g) to mix the sample, and carry out normal phase silica gel column chromatography separation (200-300 mesh, 1kg; silica gel column size L 500mm, ), followed by gradient elution with chloroform / methanol with a volume ratio of 9:1, 5:1, 3:1, 1:1, 1:3, 1:9, 5L each time; TLC detected fractions, and collected the elution ratio The 3:1 fractions were combined and recrystallized from methanol.

Embodiment 2

[0027] Example 2 Preparation of polyphenol diglycoside compound

[0028] Take 5kg of Zhulan whole herb, dry, crush, extract with 5L of ethanol under reflux, concentrate the extract, suspend with 1L of distilled water, extract the aqueous suspension with 1L of chloroform, and discard the chloroform layer. The aqueous layer was extracted with 1L n-butanol; the n-butanol was partially concentrated, and the sample was mixed with silica gel (100 mesh, 100 g), and separated by normal phase silica gel column chromatography (200-300 mesh, 1 kg; silica gel column size L 500mm, ), followed by gradient elution with chloroform / methanol with a volume ratio of 9:1, 5:1, 3:1, 1:1, 1:3, 1:9, 5L each time; TLC detected fractions, and collected the elution ratio The 3:1 fractions were combined and recrystallized from methanol.

Embodiment 3

[0029] Example 3 Preparation of polyphenol diglycoside compound

[0030] Take 5kg of Zhulan whole herb, pulverize it, extract with 5L of methanol / ethanol mixture, concentrate the extract, mix the sample with 100g of diatomaceous earth, heat extract with 5L of n-butanol; carry out normal phase silica gel column chromatography (200-300 mesh , 1kg; silica gel column size L 500mm, ), followed by gradient elution with chloroform / methanol mixtures with a volume ratio of 9:1, 5:1, 3:1, 1:1, 1:3, and 1:9, 5 L each time; Fractions with a separation ratio of 3:1 were combined and then recrystallized with acetone / n-hexane (volume ratio 1:1).

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
sizeaaaaaaaaaa
Login to View More

Abstract

A polyphenol diglucoside compound, a preparing method thereof and applications of the compound are disclosed. The structure of the polyphenol diglucoside compound is shown as a formula (I). The preparing method includes putting smashed whole plant of chloranthus spicatus (Thunb.) makino into an organic solvent, digesting, separating to obtain leach liquor, extracting the leach liquor, concentrating extract liquid to obtain an extract, and subjecting the extract to chromatographic separation for purification to obtain the polyphenol diglucoside compound. The organic solvent is one or a mixture of a plurality of solvents selected from methanol, ethanol, n-butanol and acetone. The polyphenol diglucoside compound having a novel structure is obtained by extracting the whole plant of the chloranthus spicatus (Thunb.) makino and separating. The compound is good in tyrosinase inhibiting activity, can be used for preparing tyrosinase inhibiting medicines, whitening cosmetics or whitening healthcare foods, and has a good development prospect.

Description

technical field [0001] The invention relates to the field of plant active ingredients, in particular to a polyphenol diglycoside compound and its preparation method and application. Background technique [0002] Zhulan (Latin scientific name: Chloranthus spicatus (Thunb.) Makino). Also known as caviar and tea orchid, it belongs to the Chrysanthemum family and Chrysanthemum genus. It is a perennial evergreen herb or semi-shrub flower. Stand upright or slightly prostrate, 30-60 cm high. The leaves are opposite, obovate-elliptic, with blunt teeth on the edge, and a gland at the tip of the teeth; the leaf surface is smooth, dark green, slightly bubbly and wrinkled. The spikes are terminal and arranged in panicles. The flowers are small, bisexual, without perianth, yellow-green, with strong orchid fragrance. The flowering period is from August to October (Chen Haishan, Cheng Yongqian, Journal of Tropical and Subtropical Botany, 1994, 2(4): 31-34.). [0003] Current research ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C07H15/04C07H1/08A61K31/7032A61K8/60A61Q19/02A23L33/105
CPCA23V2002/00A23V2200/318A61K8/602A61Q19/02C07H1/08C07H15/04
Inventor 吴斌
Owner ZHEJIANG UNIV
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com