A kind of Lactobacillus plantarum with the function of alleviating pfos poisoning and its application
A technology of Lactobacillus plantarum and drugs, applied in the field of microorganisms, can solve problems such as no development of toxic damage, and achieve the effect of good adsorption capacity
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0103] Example 1 : Tolerance of Lactobacillus plantarum CCFM737 to PFOS
[0104] The tolerance of Lactobacillus plantarum CCFM737 to PFOS was determined by the minimum inhibitory concentration (MIC) method. Prepare a 100 mg / L PFOS solution as the mother solution of PFOS, and then filter it with a sterile filter with a pore size of 0.22 μm. Add the sterilized PFOS mother solution in proportion to the MRS agar medium and mix well so that the final concentrations are 1.0, 2.0, 5.0, 10, 20, 30, 50 mg / L, and pour the plates respectively. Divide each agar plate into four equal parts, add 10 μL of Lactobacillus plantarum CCFM737 suspension to each partition, make 2 parallel plates for each PFOS concentration, and use the agar plate without PFOS as a blank control . After incubation at 37°C for 48 h, the growth of the dropwise bacteria in each partition was recorded. The lowest PFOS concentration that inhibits the growth of the strain is the PFOS MIC of the strain. Thus, the MIC...
Embodiment 2
[0107] Example 2 : Adsorption capacity experiment of Lactobacillus plantarum CCFM737 on PFOS
[0108] Incubation in PFOS-containing aqueous solution in vitro has good adsorption capacity for PFOS
[0109]Bacterial adsorption: Under aseptic conditions, according to the screening criteria of acid resistance (growth at pH 3.0), 10 strains of lactic acid bacteria were screened from traditional Chinese foods, such as kimchi and fermented milk wine. After purifying and activating the 10 strains of lactic acid bacteria, they were inoculated into MRS liquid medium according to 1% (v / v) inoculation amount, and cultivated at 37°C for 20h. Then centrifuge under the condition of 10000r / min for 20min, take the precipitate and wash it with ultrapure water, and then continue to centrifuge for 20min under the condition of 10000r / min, take the precipitate to obtain live bacterial cells, that is, wet bacterial cells. The wet bacteria were resuspended in 50mg / L PFOS solution, and the final ba...
Embodiment 3
[0117] Example 3 : Tolerance Dose Experiment of Lactobacillus plantarum CCFM737 Feeding Mice
[0118] The freeze-dried powder of Lactobacillus plantarum CCFM737 was resuspended in skim milk to make a concentration of 5.0×10 9 cfu / mL suspension. Take 10 healthy male C57BL / 6J mice of about 20-25g, orally administer the suspension of this concentration once a day, observe for a week, and record the death and body weight.
[0119] The results of these tests are listed in Appendix Table 3. These results indicated that feeding concentrations of 5.0×10 9 The cfu / mL Lactobacillus plantarum CCFM737 had no significant effect on the mice, the body weight increased significantly, and no death occurred. The appearance of the mice had no obvious pathological symptoms.
[0120] Table 3 Effect of feeding Lactobacillus plantarum CCFM737 on body weight and survival rate of mice
[0121]
[0122] Note: -: no death of mice
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More