A kind of cell model and screening method for screening CCR4 antagonists

A screening method and technology for antagonists, applied in the field of medicine and cell biology, can solve the problems of difficulty, low safety, and small test window of antagonists
CN105462929BActive Publication Date: 2019-11-22INST OF PHARMACOLOGY & TOXICOLOGY ACAD OF MILITARY MEDICAL SCI P L A
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
INST OF PHARMACOLOGY & TOXICOLOGY ACAD OF MILITARY MEDICAL SCI P L A
Publication Date
2019-11-22

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Abstract

The present invention belongs to the field of pharmacy and cell biology, and relates to an efficient and reliable high content CCR4 antagonist screening cell model using human chemokine receptor 4 as a target, and a screening method thereof. According to the present invention, a cell line being subjected to human CCR4-enhanced green fluorescent protein fusion expression introducing is established, excitation is performed through human thymus activation regulated chemokine and human macrophage chemotatic factor, the CCR4 with green fluorescent protein is induced to produce re-distribution so as to form green fluorescent particles, and the CCR4 antagonist screening cell model for high-content screening is established; the bioactivity of the compound is evaluated by determining the CCR4 green fluorescent particle formation excited or inhibited by the screened compound through the model; and the established drug screening model is the sensitive, efficient and reliable CCR4 antagonist screening cell model suitable for high-throughput and high-content screening, and the method is the sensitive, efficient and reliable method suitable for high-throughput and high-content screening.
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Description

technical field

[0001] The invention belongs to the field of medicine and cell biology, and relates to a high-throughput screening cell model of CCR4 antagonists and its establishment method and application, in particular to a CCR4-based high-content screening CCR4 antagonist cell model and its establishment method and application. Background technique

[0002] Drug screening models are an essential platform for drug activity research in drug development. High-content analysis technology can simultaneously detect the influence of the tested sample on cell morphology, growth, differentiation, migration, apoptosis, metabolic pathway and signal transduction under the premise of maintaining the integrity of cell structure and function. At the same time, it is possible to observe the relevant toxicity information of the test sample. Therefore, the introduction of high-content screening technology is the trend of innovative drug screening research.

[0003] CCR4 (chemokine recep...

Claims

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