Sample pretreatment method for extracting microorganism total DNA in biological aerosol
A sample pretreatment and bioaerosol technology, which is applied in the field of microbial research in air aerosol, can solve the problems of complex buffer configuration process, large DNA loss, large filter membrane area, etc. Effects of poisoning and simplification of operation steps
Active Publication Date: 2016-04-06
BEIJING AGRO BIOTECH RES CENT
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Abstract
The invention relates to a sample pretreatment method for extracting microorganism total DNA in biological aerosol. The method includes the steps that a filter membrane is adopted to collect air microorganisms, 0.8% normal saline is used as a buffer solution, the microorganisms are fully separated under the oscillation action of a shaking table supplemented by glass beads, and membrane stripping liquid is filtered through a 25-mm filter membrane, so that the microorganisms are concentrated on the surface of a membrane with the area of 1.96 cm<2> and oscillated, walls of the microorganisms, with thick cell walls, on the surface of the membrane are broken preliminarily, and follow-up DNA extraction is facilitated. The sample pretreatment method is easy to operate, harmless to the human body and capable of effectively increasing the DNA extraction amount.
Application Domain
DNA preparation
Technology Topic
Saline waterChemistry +10
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- Experimental program(2)
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Example Embodiment
PUM
Property | Measurement | Unit |
Diameter | 5.0 | mm |
Diameter | 25.0 | mm |
Aperture | 0.22 | µm |
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