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A kind of extraction method of high-purity anthocyanin

An extraction method, anthocyanin technology, applied in the direction of chemical instruments and methods, azo dyes, organic dyes, etc., can solve the problems of extraction and purification limitations, anthocyanin instability, etc., to achieve stability, low price, Achieve pollution-free effect

Active Publication Date: 2017-08-25
丁新华
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, since anthocyanins are unstable under light, heat and alkaline conditions, and stable under weak acid conditions, this makes the extraction and purification of anthocyanins subject to certain conditions, which makes the extraction and purification of anthocyanins a current Emerging Frontiers of Research Topics

Method used

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  • A kind of extraction method of high-purity anthocyanin
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  • A kind of extraction method of high-purity anthocyanin

Examples

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Embodiment 1

[0037] A kind of extraction method of high-purity purple tomato anthocyanin of the present embodiment, it comprises the following steps:

[0038] (1) Pretreatment of raw materials: Remove the petiole and wash the purple tomato fruit that has been stored at low temperature and thawed, weigh 2.2kg, then cut into pieces and homogenize with a juicer.

[0039] (2) Acid water extraction: first prepare the hydrochloric acid aqueous solution extract with pH=1 according to the ratio of 1:3 (2.2kg tomato fresh weight corresponds to 6.6L pH=1 hydrochloric acid water), and then extract the purple tomato in step (1) The pomace after juicing is ultrasonically extracted with the prepared acid water, the ultrasonic temperature is 20-25°C, the ultrasonic power is 500-700W, ultrasonic 3 times, the time is 30min each time, and after three times, it is filtered with 200 mesh gauze to obtain the pomace. extract; at the same time, adjust the pH=1 with the hydrochloric acid used for the homogenized ...

Embodiment 2

[0047] A kind of extraction method of high-purity solanum anthocyanins of the present embodiment, it comprises the following steps:

[0048] (1) Pretreatment of raw materials: Remove the petiole and wash the fresh nightshade fruit that has been stored at low temperature and thawed, weigh 950g, squeeze the juice with a juicer, and separate the juice and pomace.

[0049] (2) Acid water extraction: first prepare a hydrochloric acid aqueous solution extract with pH=1 at a ratio of 1:3 (0.95kg tomato fresh weight corresponds to 2.85L pH=1 hydrochloric acid water), and then extract the purple tomato in step (1) The pomace after juicing is ultrasonically extracted with the prepared acid water, the ultrasonic temperature is 20-25°C, the ultrasonic power is 500-700W, ultrasonic 3 times, the time is 30min each time, and after three times, it is filtered with 200 mesh gauze to obtain the pomace. extract; at the same time, adjust the pH=1 with the hydrochloric acid used for the homogenize...

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Abstract

The invention relates to a method for extracting high purity anthocyanin. According to the method, purple tomatoes and nightshade are utilized as raw materials respectively. The purple tomatoes and the nightshade are smashed and homogenated first and then are mixed with acid water with the pH of 1 and undergo supersonic extraction and filtration, filter liquor is adsorbed by macroporous resin, impurities are removed, the acid with the pH of 1 and 95% ethyl alcohol are utilized to perform elution, and obtained elution liquid undergoes mist spray and drying by a spray dryer to obtain high purity anthocyanin powder. According to the method, the problems that the loss is large, the yield is low, and the purity is low in the extracting process of plant anthocyanin are solved, the purity of the obtained purple potato anthocyanin reaches up to 54%, the yield accounts for 0.48% of fresh weight, the purity of nightshade anthocyanin reaches up to 56%, and the yield reaches up to 1.3% of the fresh weight. The problem that the purity of the plant anthocyanin sold in the domestic market is generally lower than 24% is solved, the device is simple to operate, the production process is clean and harmless, the product quality is stable, and the method is suitable for industrial production.

Description

technical field [0001] The invention relates to the technical field of extracting natural active substances, in particular to a method for extracting high-purity anthocyanins, so as to realize high-efficiency extraction and purification of plant anthocyanins. Background technique [0002] Anthocyanin is a natural water-soluble plant pigment belonging to flavonoid compounds. It mainly exists in plant cell vacuoles in the form of glycosides combined with sugars. The main color-forming component is methylcyanidin acyl glucoside of anthocyanins. And cyanidinyl glucoside, precisely because anthocyanins have acyl and glycosyl structures, the stability during pigment extraction is improved, and the physiological health care skills are also enhanced. It can be widely used in the fields of health care products, food and cosmetics, and it is also very effective in anti-oxidation, anti-mutation, anti-inflammation, anti-allergic, diuretic, antispasmodic, antitussive, blood lipid lowerin...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C09B61/00C07D311/62
CPCC07D311/62C09B61/00
Inventor 丁新华储昭辉汪少丽李明赵长宝
Owner 丁新华
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