Production technology for high-density culture of chlorella by utilizing fermentation method

A production process and fermentation culture technology, which is applied in the production process field of high-density cultivation of chlorella by fermentation method, can solve the problems of easy pollution, low quality, and low density of algae cells, so as to reduce production costs, increase protein content, and shorten abnormal The effect of the incubation period

Inactive Publication Date: 2016-04-13
QINGDAO KEHAI BIOLOGICAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] At present, the large-scale cultivation of chlorella mainly adopts outdoor open ponds, which have problems such as low algae cell density, low quality, and easy pollution.

Method used

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  • Production technology for high-density culture of chlorella by utilizing fermentation method
  • Production technology for high-density culture of chlorella by utilizing fermentation method
  • Production technology for high-density culture of chlorella by utilizing fermentation method

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Effect test

Embodiment 1

[0042] The present invention, the production technique of chlorella, specifically adopts following method:

[0043] (1) Put the activated Chlorella algae and the newly connected shake flask into the shaker for light culture. After 3 days of normal culture, insert it into the 5L shake flask according to the subculture operation and cultivate it for 2 generations. Continue to pass on to the third generation. During generation, cultivate under light in a shaker, and after 3 days of normal culture, the concentration of the algae liquid reaches 10g / L;

[0044] (2) Step (1) is used to cultivate the chlorella seed solution in the logarithmic phase of growth, and insert it into a 150L seed tank by 10% (v / v) to carry out different culture;

[0045] 150L seed tank with fixed capacity of 100L,

[0046] The medium formula is:

[0047] Glucose: 30-60g / L, magnesium sulfate: 0.5-1.6g / L, calcium citrate: 0.1-0.5g / L, sodium phosphate: 1.0-2.0g / L, dipotassium hydrogen phosphate: 0.3-0.8g / L ,...

Embodiment 2

[0078] The present invention, the production technique of chlorella, specifically adopts following method:

[0079] (1) Put the activated Chlorella algae and the newly connected shake flask into the shaker for light culture. After 3 days of normal culture, insert it into the 5L shake flask according to the subculture operation and cultivate it for 2 generations. Continue to pass on to the third generation. During the generation, culture in the shaker under light, and after 3 days of normal culture, the concentration of the algae liquid reaches 8g / L;

[0080] (2) The chlorella seed solution cultivated in step (1) to the logarithmic phase of growth is inserted into a 150L seed tank at 10% (v / v) for abnormal cultivation;

[0081] 150L seed tank with fixed capacity of 100L;

[0082] The medium formula is as follows:

[0083] Glucose: 30-60g / L, magnesium sulfate: 0.5-1.6g / L, calcium citrate: 0.1-0.5g / L, sodium phosphate: 1.0-2.0g / L, dipotassium hydrogen phosphate: 0.3-0.8g / L , f...

Embodiment 3

[0114] The present invention, the production technique of chlorella, specifically adopts following method:

[0115] (1) Put the activated Chlorella algae and the newly connected shake flask into the shaker for light culture. After 3 days of normal culture, insert it into the 5L shake flask according to the subculture operation and cultivate it for 2 generations. Continue to pass on to the third generation. During the generation, culture in the shaker under light, after 3 days of normal culture, the concentration of the algae liquid reaches 11g / L;

[0116] (2) Step (1) is used to cultivate the chlorella seed solution in the logarithmic phase of growth, and insert it into a 150L seed tank by 10% (v / v) to carry out different culture;

[0117] 150L seed tank with fixed capacity of 100L;

[0118] The medium formula is as follows:

[0119] Glucose: 30-60g / L, magnesium sulfate: 0.5-1.6g / L, calcium citrate: 0.1-0.5g / L, sodium phosphate: 1.0-2.0g / L, dipotassium hydrogen phosphate: 0....

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Abstract

The invention discloses a production technology for high-density culture of chlorella by utilizing a fermentation method. The production technology is characterized in that firstly, chlorella seed culture is carried out through heterotrophic cultivation, in the fourth stage of the heterotrophic cultivation process, a basal culture medium and a fed-batch culture medium are selected and used for co-culture, and the two media are utilized in combination with fermentation tanks to perform high-density heterotrophic cultivation on chlorella pyrenoidosa; besides, by adopting a semi-continuous fed-batch cultivation method and measuring the growth rate, dry weight, protein content and the like of chlorella, the optimum culture condition for maintaining of the stable growth of chlorella strains is determined; the experimental result shows that the pH is 5.5-7.5, DO is larger than or equal to 20%, the best ratio of carbon to nitrogen C/N for feed-batch culture equals to 100, the suitable carbon concentration for feed-batch culture is 40-60 g/L, the reclaiming rate is 1/5, chlorella can realize high-density stable growth, and after 4-5 days' fermentation, the dry weight can reach 100 g/L or above, and the protein content can reach 58 g/L or above. The production technology not only realizes pure culture, but also improves the algae quality to a greater extent; besides, chlorella prepared by adopting the production technology contains other multivitamins at the same time, and further meets the nutritional requirements of human bodies.

Description

technical field [0001] The invention relates to the technical field of large-scale cultivation of chlorella, in particular to a production process for high-density cultivation of chlorella by a fermentation method. Background technique [0002] Chlorella is a general unicellular algae of the genus Chlorella in the Chlorophyta phylum. It is a simple photosynthetic organism. The most common Chlorella pyrenoidosa is commercially exploited for its rich protein Contains a variety of vitamins, polysaccharides, unsaturated fatty acids, amino acids, dietary fiber, trace elements and other active substances, with anti-tumor, anti-radiation, anti-infection, anti-atherosclerosis, lowering blood pressure, lowering blood lipids, preventing anemia and other series It is one of the microalgae that can be commercially produced in the current health food application. [0003] At present, the large-scale cultivation of chlorella mainly adopts outdoor open ponds, which have problems such as l...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12R1/89
CPCC12N1/12
Inventor 李悦明夏修峦徐建春崔建新李霞王道会迟慎丽孙慧彬赵守鹏赵世宝王彬崔显俊
Owner QINGDAO KEHAI BIOLOGICAL
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