Method for synthesizing Sn-2-monoglyceride through enzymic method

An enzymatic synthesis, sn-2- technology, applied in fermentation and other directions, can solve the problems of high cost and low yield, and achieve the effects of high conversion rate, fast reaction rate and mild reaction conditions

Active Publication Date: 2016-04-13
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the yield of this process is low and the cost is high

Method used

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  • Method for synthesizing Sn-2-monoglyceride through enzymic method
  • Method for synthesizing Sn-2-monoglyceride through enzymic method
  • Method for synthesizing Sn-2-monoglyceride through enzymic method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] The batch enzyme reaction is carried out in a batch-type stirred tank reactor, the mass ratio of high oleic sunflower oil and ethanol is 1:5, 8% immobilized lipase Lipozyme435 is added in a solvent-free system, and stirred at normal pressure 25 ° C Reaction for 3 hours, stirring speed is 400r / min, after the reaction is over, centrifuge at 4000r / min for 10min to remove lipase, and obtain Sn-2-monoglyceride rich in oleic acid. After HPLC analysis, Sn-2-monoglyceride The yield was 93.26%.

Embodiment 2

[0032] The batch enzyme reaction was carried out in a batch-type stirred tank reactor, the mass ratio of tea seed oil and ethanol was 1:7, 5% immobilized lipase Novozym435 was added in a solvent-free system, and the reaction was stirred for 4 hours at normal pressure 30°C , stirring speed is 300r / min, after the reaction finishes, centrifuge 10min under the condition of 4000r / min to remove lipase, obtain the Sn-2-monoglyceride rich in oleic acid, through HPLC analysis, the obtaining of Sn-2-monoglyceride The rate is 88.15%.

Embodiment 3

[0034] The batch enzyme reaction was carried out in a batch stirred tank reactor with fish oil and ethanol at a mass ratio of 1:4, the added reactant to acetone at a ratio of 1:1 (w / v), and 10% immobilized fat Enzyme NS40086, stirred and reacted at normal pressure 20°C for 2 hours, and the stirring speed was 500r / min. After the reaction, centrifuged at 4000r / min for 10 minutes to remove lipase, and obtained Sn-2-monoglyceride. After HPLC analysis, Sn-2 - The yield of monoglycerides is 15.82%.

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Abstract

The invention discloses a method for synthesizing Sn-2-monoglyceride through an enzymic method. The method comprises the steps that glyceride and ethyl alcohol are mixed according to the mass ratio of 1:(1-10), in a solvent-free system, lipase is added, reacting under stirring is performed for a period of time at a certain temperature, an ethanolysis reaction is performed, and a product containing the Sn-2-monoglyceride is obtained. According to the method, an acyl transfer side reaction is inhibited through the specificity and high efficiency of enzymes by controlling the reaction conditions, and the reaction conversion rate is very high; meanwhile, the synthesizing time is shortened, the whole process is easy to control, the reaction conditions are mild, the cost can be greatly lowered through recycling of the enzymes, large-scale industrialization promotion is facilitated, and the application potential of the method is great.

Description

technical field [0001] The invention belongs to the technical field of oil synthesis, and in particular relates to a method for enzymatically synthesizing Sn-2-monoglyceride. Background technique [0002] Monoglyceride is a nonionic surfactant. It has both hydrophilic and lipophilic genes, and has multiple functions such as wetting, emulsifying, and foaming. Monoglyceride is used as an emulsifier in cosmetics and pharmaceutical ointments to make the paste delicate and smooth; it is used as an emulsifier for industrial silk oils and a lubricant for textiles; it is used as a dripping agent and anti-fog agent in plastic films; It can be used as lubricant and antistatic agent in plastic processing, and can be used as defoamer, dispersant, thickener, wetting agent, etc. in other aspects. Especially in food production, it is widely used as an emulsifier and is the largest food emulsifier in the world. At the same time, Sn-2-monoglyceride can also synthesize functional and symme...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/64
CPCC12P7/6445
Inventor 王小三陆继源王盈盈黄健花金青哲王兴国
Owner JIANGNAN UNIV
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