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Drop array chip and preparation method thereof

An array chip and droplet technology, applied in the field of biochemical analysis, can solve the problems of high cost, unstable flow rate, complex processing technology, etc., and achieve the effects of accurate and efficient morphology, excellent performance and simple operation.

Inactive Publication Date: 2016-04-20
SHENZHEN INST OF ADVANCED TECH CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method is not only complicated in processing technology, but also requires auxiliary equipment such as microfluidic drivers and droplet loaders. It is difficult to effectively control the size and uniformity of microdroplets, and the cost is high and the operation is inconvenient.
On the other hand, microfluidic chip droplet technology cannot form any droplet structure with any shape and arrangement due to the limitations of microchannels, and it is prone to adverse phenomena such as channel blockage and unstable flow rate.
In addition, it is difficult to realize the generation, capture, screening and analysis of large quantities of droplets at the same time based on the technical approaches of droplet generation such as electrical screening, optical manipulation and pneumatic valve technology, which limits its wide application in practice.

Method used

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  • Drop array chip and preparation method thereof
  • Drop array chip and preparation method thereof
  • Drop array chip and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] droplet array chip

[0056] A droplet array chip, comprising: a substrate; the substrate has hydrophilic regions and hydrophobic regions arranged alternately to form a hydrophilic-hydrophobic array;

[0057] The hydrophilic region is formed by oxygen plasma treatment; the hydrophobic region is formed by physical volatilization deposition of perfluorodecyltriethoxysilane; the hydrophilic and hydrophobic array is a planar droplet array; the hydrophilic region is A square array with a side length of 200um and a pitch of 400um;

[0058] The base material is single crystal silicon.

[0059] Preparation method of droplet array chip

[0060] A method for preparing a droplet array chip, comprising the following steps:

[0061] 1) Take a substrate and clean its surface to remove surface organic and inorganic impurities; the specific cleaning steps are: sequentially ultrasonically clean in acetone, absolute ethanol and ultrapure water, blow dry with nitrogen and put it in an...

Embodiment 2

[0069] droplet array chip

[0070] Except that the shapes of the hydrophilic and hydrophobic regions are different and the positions of the hydrophilic and hydrophobic regions are replaced with each other, the rest are the same as in Example 1; the hydrophobic regions of the droplet array chip in this embodiment are circular, with a diameter of 20um and a pitch of 200um. array arrangement.

[0071] Preparation method of droplet array chip

[0072] Except that the mask plate in step b is arranged in a circular array with a diameter of 20 um and a pitch of 200 um, and a circular concave array pattern surrounded by photoresist is obtained by developing, the rest is the same as that of embodiment 1.

[0073] The above-mentioned droplet array chip is immersed in hexadecene, and the droplet array formed by hexadecene droplets is obtained after taking it out, as shown in image 3 shown.

Embodiment 3

[0075] droplet array chip

[0076] Except that the shapes of the hydrophilic and hydrophobic regions are different and the positions of the hydrophilic and hydrophobic regions are replaced with each other, the rest are the same as in Embodiment 1; the hydrophobic region of the droplet array chip in this embodiment is a square array with a side length of 100um and a pitch of 200um;

[0077] Preparation method of droplet array chip

[0078] A method for preparing a droplet array chip, comprising the following steps:

[0079] 1) Take a substrate and clean its surface to remove surface organic and inorganic impurities; the specific cleaning steps are: sequentially ultrasonically clean in acetone, absolute ethanol and ultrapure water, blow dry with nitrogen and put it in an oven for drying. Remove the water vapor attached to the substrate itself; the substrate material is monocrystalline silicon;

[0080] 2) Treat the substrate to obtain the affinity array

[0081] a. Carry ...

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Abstract

The invention discloses a drop array chip which comprises a substrate. The substrate is provided with hydrophilic areas and hydrophobic areas, and the hydrophilic areas and the hydrophobic areas are alternately arranged to form a hydrophilic-hydrophobic array. The invention further discloses a preparation method of the drop array chip. According to the drop array chip, a large number of array type drop structures with uniform and controllable morphologies can be precisely and efficiently generated through the hydrophilic-hydrophobic property of the surface of the drop array chip, a foundation is provided for complex biochemical application based on drops, and meanwhile the advantages of being low in cost and capable of being reused are achieved.

Description

technical field [0001] The invention belongs to the field of biochemical analysis, in particular to a droplet array chip and a preparation method thereof. Background technique [0002] In recent years, micro-total analysis systems based on droplet arrays have been widely used in the fields of life analysis chemistry research due to their advantages of miniaturization, integration, automation, throughput, speed, sensitivity, and portability. Many typical experimental operations, Such as mixing, stirring, sampling, metering, etc. can all be carried out in the droplet array, and multi-component simultaneous detection can be realized. At present, a variety of droplet array chips have been put into practical use, such as for the preparation of sample DNA, PCR reaction, electrophoresis detection, electrospray ion mass spectrometry detection, etc. [0003] However, at present, most commonly used droplet structure generation methods are microfluidic chip droplet technology, such as...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/00G01N27/447G01N27/62
CPCB01J19/0093G01N27/447G01N27/62C12N15/1006B01J2219/00317B01J2219/00608B01J2219/00529B01J2219/00722
Inventor 杜学敏王娟吴天准唐天洪崔欢庆
Owner SHENZHEN INST OF ADVANCED TECH CHINESE ACAD OF SCI
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