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Porcine reproductive and respiratory syndrome virus diluent and preparation method thereof

A respiratory syndrome and diluent technology, applied in biochemical equipment and methods, viruses, viruses/phages, etc., can solve the problems of increasing production costs, reducing the immunogenicity of PRRS virus, etc., to increase the virus content, maintain The effect of normal cell growth and increased virus titer

Inactive Publication Date: 2016-04-20
浙江美保龙生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, in order to increase the virus content, the usual practice is to concentrate the PRRS antigen by ten times or even dozens of times, which not only increases the production cost, but also reduces the immunogenicity of the PRRS virus during the concentration process.

Method used

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  • Porcine reproductive and respiratory syndrome virus diluent and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] A dilution of porcine reproductive and respiratory syndrome virus, which contains per 1000ml of phosphate buffer: 20g of amino acid combination (4g of folic acid, 6g of riboflavin sodium phosphate, 4g of dexpanthenol, 6g of ascorbic acid), 20g of compound Chinese medicine polysaccharide and liposome 20g, the pH of the diluent is 7.2.

[0041] A preparation method of porcine reproductive and respiratory syndrome virus dilution, the steps are:

[0042](1) Preparation of phosphate buffer solution: Accurately weigh 8 g of sodium chloride, 0.2 g of potassium chloride, 1.15 g of disodium hydrogen phosphate, 0.2 g of potassium dihydrogen phosphate, and 0.1 g of calcium chloride according to the above formula of phosphate buffer solution. Dissolve 0.1 g of magnesium chloride containing 6 crystal waters in 1000 ml of double distilled water, pass through a 0.22 μm filter membrane under aseptic conditions, and filter and sterilize for later use.

[0043] (2) Preparation of compou...

Embodiment 2

[0055] A porcine reproductive and respiratory syndrome virus dilution, the virus dilution per 1000ml phosphate buffer (containing sodium chloride 6g, potassium chloride 0.05g, disodium hydrogen phosphate 1g, potassium dihydrogen phosphate 0.05g, calcium chloride 0.05 g, magnesium chloride containing 6 crystal water 0.05g) contains: amino acid combination 30g (folic acid 12g, riboflavin sodium phosphate 3g, dexpanthenol 3g, ascorbic acid 12g), compound Chinese medicine polysaccharide 30g (congduhuo 10 parts, Yun vanilla 20 parts, 25 parts of Vitex, 25 parts of Budisma japonica, 30 parts of Radix Scrophulariae and 25 parts of Radix Paeoniae Rubra), liposome 25g (prepared according to Example 1), and the pH of the dilution is 7.5.

[0056] A dilution of porcine reproductive and respiratory syndrome virus and a preparation method thereof, the steps of which are the same as in Example 1.

Embodiment 3

[0058] A porcine reproductive and respiratory syndrome virus dilution and preparation method thereof, the virus dilution per 1000ml phosphate buffer (containing 10g of sodium chloride, 0.5g of potassium chloride, 1.2g of disodium hydrogen phosphate, 0.5g of potassium dihydrogen phosphate , calcium chloride 0.2g, magnesium chloride containing 6 crystal water 0.2g) contains: amino acid combination 10g (folic acid 2g, riboflavin sodium phosphate 2.5g, dexpanthenol 1.5g, ascorbic acid 4g), compound Chinese medicine polysaccharide 25g ( Extracted from 20 parts of lovage, 20 parts of Yunxiangcao, 25 parts of Vitex, 25 parts of Budisma japonica, 30 parts of Radix Scrophulariae and 25 parts of Radix Paeoniae Rubra), 10 g of liposomes (prepared according to Example 1), the diluted The liquid pH was 7.4.

[0059] A dilution of porcine reproductive and respiratory syndrome virus and a preparation method thereof, the steps of which are the same as in Example 1.

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Abstract

The invention provides a porcine and respiratory syndrome virus diluent and a preparation method thereof and belongs to the technical field of veterinary biological products. Every 1000 ml phosphate buffer of the diluent is prepared from, by weight, 10-30 g of amino acid combination, 10-30 g of compound traditional Chinese medicine polysaccharides and 10-30 g of liposome, wherein the amino acid combination comprises folic acid, riboflavin sodium phosphate, dexpanthenol and ascorbic acid, and the compound traditional Chinese medicine polysaccharides are extracted from pubescent angelica roots, Cymbopogon distans (Nees)A. Camus, chastetree fruit, butterflybush flowers, radix scrophulariae and red peony roots. The diluent has the advantages that after virus inoculation, cell growth can be promoted, and normal growth of cells can be maintained; viruses can infect the cells more easily after processing, and the probability that the viruses infect the cells is greatly increased; virus titer can be greatly increased in the same culture time, and virus content is increased by 10-100 times.

Description

technical field [0001] The invention belongs to the technical field of veterinary biological products, and in particular relates to a diluent of porcine reproductive and respiratory syndrome virus and a preparation method thereof. Background technique [0002] Pig blue-ear disease, also known as porcine reproductive and respiratory syndrome (Porcinereproductiveandrespiratorysyndrome, PRRS), is caused by porcine reproductive and respiratory syndrome virus (Porcinereproductiveandrespiratorysyndromevirus, PRRSV), and pregnant sows have premature birth, abortion, stillbirth, weak piglets and Disease with respiratory symptoms in mummified fetuses, piglets and finishing pigs. It is difficult to effectively prevent and control PRRS. Firstly, PRRSV targets lung macrophages, which has antibody-dependent enhancement (ADE), causing certain immunosuppression. Secondly, PRRSV is constantly mutating, and there is no effective treatment for PRRS Drugs and immunization are currently the mo...

Claims

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Application Information

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IPC IPC(8): C12N7/00C12R1/93
CPCC12N7/00C12N2770/10051
Inventor 沈建军张秀文李阳
Owner 浙江美保龙生物技术有限公司
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