Ramie biological degumming method

A biological degumming and ramie technology, which is applied in the chemical post-treatment of plant raw materials, textiles and papermaking, and fibers, can solve the problems of harsh process conditions, ramie degumming pollution, and low product quality, and achieve low residual glue rate and degumming The effect of high efficiency and low cost

Active Publication Date: 2016-04-27
HUAZHONG UNIV OF SCI & TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The technical problem to be solved by the present invention is to provide a biological degumming method for ramie to solve the problems of serious pollution, harsh process conditions, long production cycle and low product quality existing in the existing ramie degumming industry

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030]Strain activation step: move the centrifuge tube containing the bacterial solution B to room temperature for 5 minutes, then inoculate the bacterial solution B into a 1L Erlenmeyer flask containing No. 200 No. 1 medium at 38 Cultivate at a temperature of 180r / min at a speed of 180r / min, and culture on a shaker for 6 hours to obtain bacterial solution C; the formula of No. 1 medium is: 10g / L of peptone and 5g / L of yeast extract powder are mixed with water per unit volume With 10g / L NaCl, and adjust the initial pH value to 7.

[0031] Expand the cultivation step: inoculate the bacterium liquid C into the 50L seed tank that 35L No. 2 medium is housed by the inoculum amount of 5%, under the temperature of 33 ℃, keep the rotating speed of 90r / min and the ventilation of 5L / min, Cultivate for 5 hours to obtain bacterial solution D; then inoculate bacterial solution D into a 1t seed tank containing 700kg of No. 3 The air flow per hour was cultivated for 2 hours to obtain bacter...

Embodiment 2

[0036] Strain activation step: move the centrifuge tube containing the bacterial solution B to room temperature for 15 minutes, then inoculate the bacterial solution B into a 1L Erlenmeyer flask containing 400mL No. 1 medium at 33 Cultivate at a temperature of 90r / min at a temperature of 90r / min, and cultivate on a shaker for 3 hours to obtain bacterial solution C; the formula of No. 1 medium is: 10g / L of peptone and 5g / L of yeast extract powder mixed with water per unit volume With 10g / L NaCl, and adjust the initial pH value to 7.2.

[0037] Expand the cultivation step: inoculate the bacterium liquid C into the 50L seed tank that 25L No. 2 medium is housed according to the inoculum amount of 1%, at the temperature of 38 ℃, keep the rotating speed of 180r / min and the ventilation of 30L / min, Cultivate for 2 hours to obtain bacterial solution D; the formula of medium No. 2 is: 10 g / L peptone, 5 g / L yeast extract powder, and 5 g / L NaCl are mixed in unit volume of water, and the i...

Embodiment 3

[0042] Strain activation step: move the centrifuge tube containing the bacterial solution B to room temperature for 10 minutes, then inoculate the bacterial solution B into a 1L Erlenmeyer flask containing 300mL No. 2 medium at 37 Cultivate at a temperature of 120r / min at a temperature of 120r / min, and cultivate on a shaker for 4 hours to obtain bacterial solution C; the formula of No. 2 medium is: 10g / L of peptone and 5g / L of yeast extract powder are mixed with unit volume of water , 5g / L of NaCl, and adjust the initial pH value to 7.7.

[0043] Expanded cultivation step: inoculate the bacterium solution C into a 50L seed tank equipped with 30L No. 2 medium by an inoculum amount of 3%, at a temperature of 37°C, maintain a rotating speed of 120r / min and an aeration of 20L / min amount, cultivated for 4 hours to obtain bacterial solution D; then inoculated bacterial solution D into a 1t seed tank containing 500kg No. 3 The ventilation rate per hour was cultivated for 4 hours to ...

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Abstract

The invention relates to a ramie biological degumming method. The method comprises carrying out activation on Bacillus sp. HG-28 as a strain, carrying out enlarge cultivation, carrying out dilution to obtain a degumming bacterial liquid F for ramie biological degumming, immersing raw ramie in the degumming bacterial liquid F and carrying out biological degumming. The ramie biological degumming method has small energy consumption, light pollution, short biological degumming time, process operation easiness, high degumming efficiency, short bacterial culture time and a low cost. The refined dry ramie has high quality, a low residual gum rate and high breaking strength, has appearance and all indexes superior to first-order refined dry ramie national standards GB / T 20793-2006 and has a wide large scale production prospect.

Description

technical field [0001] The invention belongs to the field of biotechnology, and more specifically relates to a method for biological degumming of ramie by using Bacillus sp.HG-28. Background technique [0002] Ramie, scientific name Boehmerianivea (L.) Gaud., is a commercial crop that provides important textile fibers. Ramie has long single fiber, high strength, fast moisture absorption and moisture dissipation, and good thermal conductivity. It is an excellent textile raw material and medical material, and has a wide range of uses. Ramie fiber can be spun purely or blended with cotton, wool, silk, etc. The fabric has the advantages of antiseptic, antibacterial, breathable, and crisp. my country is a big producer of ramie fiber, and its output accounts for more than 95% of the world. It has a great demand in the international market, and it is a characteristic industry with competitive advantages in the international market. [0003] At present, the main bottleneck problem...

Claims

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Application Information

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IPC IPC(8): D01C1/00
Inventor 余龙江樊培杨英何峰敖明章胡祖德胡振华
Owner HUAZHONG UNIV OF SCI & TECH
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