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Method for preparing phase-separation giant phospholipid vesicle array by using point-surface electrode electric field and on basis of micro contact stripping technique

A point-surface electrode and giant phospholipid technology, which is applied in the field of preparing phase-separated giant phospholipid vesicle arrays, can solve the problems that phase-separated giant phospholipid vesicle arrays cannot be prepared, and achieve clear phase separation, simple operation, and excellent reaction conditions warm effect

Active Publication Date: 2016-04-27
HARBIN INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The purpose of the present invention is to solve the existing problem that phase-separated giant phospholipid vesicle arrays cannot be prepared, and to provide a method for preparing phase-separated giant phospholipid vesicle arrays based on micro-contact stripping technology using point-surface electrode electric field

Method used

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  • Method for preparing phase-separation giant phospholipid vesicle array by using point-surface electrode electric field and on basis of micro contact stripping technique
  • Method for preparing phase-separation giant phospholipid vesicle array by using point-surface electrode electric field and on basis of micro contact stripping technique
  • Method for preparing phase-separation giant phospholipid vesicle array by using point-surface electrode electric field and on basis of micro contact stripping technique

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specific Embodiment approach 1

[0027] Specific embodiment 1: This embodiment is a method for preparing phase-separated giant phospholipid vesicle arrays based on micro-contact stripping technology using the electric field of point-surface electrodes, which is completed according to the following steps:

[0028] 1. Clean the electrode: first use absolute ethanol to ultrasonically clean the ITO electrode, then use distilled water to ultrasonically clean the ITO electrode, then use nitrogen to dry it, and then use a plasma cleaner to clean the ITO electrode; the cleaned ITO electrode is obtained Electrode: Soak the tungsten needle electrode in hydrochloric acid with a substance concentration of 2mol / L for 3h to 6h, then rinse it with distilled water for 3 to 6 times, and then dry it with nitrogen to obtain the cleaned tungsten needle electrode;

[0029] 2. Cleaning of polydimethylsiloxane stamps with patterns: First, use distilled water to ultrasonically clean the polydimethylsiloxane stamps with patterns, and ...

specific Embodiment approach 2

[0047] Specific embodiment 2: The difference between this embodiment and specific embodiment 1 is that the number of ultrasonic cleaning of the ITO electrode described in step 1 using absolute ethanol is 2 to 3 times, and the time for each ultrasonic cleaning is 5 minutes. ~15min, the ultrasonic power is 40W~80W. Other steps are the same as in the first embodiment.

specific Embodiment approach 3

[0048] Embodiment 3: The difference between this embodiment and Embodiment 1 or 2 is that the number of ultrasonic cleaning of the ITO electrode with distilled water described in step 1 is 2 to 3 times, and the time for each ultrasonic cleaning The time is 5min~15min, and the ultrasonic power is 40W~80W. Other steps are the same as those in Embodiment 1 or 2.

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Abstract

The invention discloses a method for preparing a phase-separation giant phospholipid vesicle array by using a point-surface electrode electric field and on the basis of a micro contact stripping technique, and relates to the method for preparing the phase-separation giant phospholipid vesicle array. The invention aims to solve the problem that a phase-separation giant phospholipid vesicle array cannot be prepared at present. The method comprises the steps: 1, cleaning an electrode; 2, cleaning a polydimethyl siloxane stamp with a pattern; 3, preparing a mixed phospholipid dry film; 4, preparing an ITO electrode of a ternary mixed phospholipid dry film array; 5, assembling an airtight preparation device; and 6, performing a phase-separation giant phospholipid vesicle array formation process, to obtain the phase-separation giant phospholipid vesicle array. The diameter of phospholipid vesicles in the prepared phase-separation giant phospholipid vesicle array is 30 [mu]m to 50 [mu]m. The method for preparing the phase-separation giant phospholipid vesicle array by using the point-surface electrode electric field and on the basis of the micro contact stripping technique is obtained.

Description

technical field [0001] The invention relates to a method for preparing phase-separated giant phospholipid vesicle arrays. Background technique [0002] Lipid rafts are microdomains containing special lipids and proteins in the lipid bilayer, which are dispersed in the cell membrane in a liquid ordered phase, and play an important role in physiological processes such as signal transduction, protein transport, cell membrane structure, and pathogen invasion in vivo. very important role. However, the lipid rafts in the cell membrane cannot be observed directly with a microscope. At present, indirect methods are mainly used to study lipid rafts. Because giant phospholipid vesicles are similar in structure to cell membranes, close in size to cells, and easy to observe with a microscope, the phase separation of ternary component giant phospholipid vesicles has been widely used in the simulation of lipid rafts. At present, the preparation of phase-separated giant phospholipid vesi...

Claims

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Application Information

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IPC IPC(8): G01N1/28
CPCG01N1/28
Inventor 韩晓军朱春桃
Owner HARBIN INST OF TECH
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