Apple seed oxidation preventing additive and preparation method thereof
An antioxidant additive, apple seed technology, applied in the functions of food ingredients, food ingredients containing natural extracts, food science and other directions, can solve problems such as harming human health, unable to provide nutrients to the human body, etc., to achieve no toxic side effects, The effect of improving antioxidant capacity and high nutritional value
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Embodiment 1
[0033] (1) Preparation of apple seed extract:
[0034] a. Dry and pulverize 1 kg of apple seeds, add 4L of 30% ethanol aqueous solution, extract under reflux at 50°C for 40 minutes, and filter and concentrate to obtain the extract;
[0035] b. Extracting the above extract with ether to obtain the extract;
[0036] c. Carry out column chromatography on the above-mentioned extract with a polyethylene glycol 5000 column, and then elute with a 5% aqueous ethanol solution to obtain the first-stage eluent;
[0037] d. The above-mentioned first-stage eluent is chromatographed with 300 mesh polyamide powder as an adsorbent, and then eluted with 0.1% acetic acid aqueous solution to obtain a second-stage eluent;
[0038] e. Recrystallizing the above-mentioned second-stage eluent twice with ethanol, and drying to obtain the apple seed extract;
[0039] (2) 80 parts by weight of the above-mentioned apple seed extract, 3 parts by weight of sodium chloride, 0.01 part by weight of selenium...
Embodiment 2
[0041] (1) Preparation of apple seed extract:
[0042] a. Dry and pulverize 1 kg of apple seeds, add 4.5 L of 40% ethanol aqueous solution, heat reflux at 55°C for 50 minutes, and filter and concentrate to obtain the extract;
[0043] b. Extracting the above extract with ether to obtain the extract;
[0044] c. Carry out column chromatography on the above-mentioned extract with a polyethylene glycol 5000 column, and then elute with an 8% ethanol aqueous solution to obtain the first-stage eluent;
[0045] d, the above-mentioned first-stage eluent is chromatographed with 300 mesh polyamide powder as an adsorbent, and then eluted with 0.2% acetic acid aqueous solution to obtain a second-stage eluent;
[0046] e. Recrystallizing the above-mentioned second-stage eluent twice with ethanol, and drying to obtain the apple seed extract;
[0047] (2) 85 parts by weight of the above-mentioned apple seed extract, 4 parts by weight of sodium chloride, 0.015 parts by weight of selenium, 0...
Embodiment 3
[0049] (1) Preparation of apple seed extract:
[0050] a. Dry and pulverize 1kg of apple seeds, add 5L of 50% ethanol aqueous solution, extract under reflux at 60°C for 60 minutes, and filter and concentrate to obtain the extract;
[0051] b. Extracting the above extract with ether to obtain the extract;
[0052] c. The above extract is subjected to column chromatography with a polyethylene glycol 5000 column, and then eluted with a 10% aqueous ethanol solution to obtain a first-stage eluent;
[0053] d, the above-mentioned first-stage eluent is chromatographed with 300 mesh polyamide powder as an adsorbent, and then eluted with 0.3% acetic acid aqueous solution to obtain a second-stage eluent;
[0054] e. Recrystallizing the above-mentioned second-stage eluent twice with ethanol, and drying to obtain the apple seed extract;
[0055] (2) 90 parts by weight of the above apple seed extract, 5 parts by weight of sodium chloride, 0.02 parts by weight of selenium, 0.2 parts by we...
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