Nano-drug carrier and drug with synergistic action of magnetic hyperthermia-chemotherapy and preparation method of nano-drug carrier and drug

A nano-drug carrier and synergistic technology, which is applied in the field of nano-drugs and corresponding preparations, to achieve the effect of promoting entry into tumor cells, reducing the killing effect, and improving the effect

Inactive Publication Date: 2016-05-11
UNIV OF SHANGHAI FOR SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the prior art has not yet provided a solution to the leak: the methods ...

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  • Nano-drug carrier and drug with synergistic action of magnetic hyperthermia-chemotherapy and preparation method of nano-drug carrier and drug
  • Nano-drug carrier and drug with synergistic action of magnetic hyperthermia-chemotherapy and preparation method of nano-drug carrier and drug
  • Nano-drug carrier and drug with synergistic action of magnetic hyperthermia-chemotherapy and preparation method of nano-drug carrier and drug

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] Preparation of γ-aminopropyltriethoxysilane-modified nano drug carrier with magnetic hyperthermia-chemotherapy synergistic effect.

[0059] Step 1: Take 0.4 gram of Fe prepared by co-precipitation method 3 o 4 Nanoparticles were ultrasonically dispersed in 36 mL of ultrapure water, and 0.6836 g of cetyl p-toluenesulfonate (CTAT) and 4 g of triethanolamine (TEA) were added to the above Fe 3 o 4 Nanoparticle solution, and stirred at 80 ° C for 1 hour; then 5.58 milliliters of tetraethyl orthosilicate (TEOS) was added quickly and continued to stir for 2 hours to obtain a tan colloidal particle solution;

[0060] Step 2: Use a magnet block to collect the tan colloidal particles in the above step 1, wash the tan colloidal particles with deionized water and ethanol several times, and then vacuum-dry them at 60°C for 24 hours. The vacuum-dried product is calcined at 550°C for 7 The organic template was removed within 1 hour to obtain the magnetic mesoporous silica nanoparti...

Embodiment 2

[0076] The nano-drug carrier provided in this example is a FITC-modified nano-drug carrier with magnetic hyperthermia-chemotherapy synergistic effect, which is used to observe the uptake of the nano-drug carrier by cells under a confocal microscope. The silane coupling agent that adopts is FITC-APTES silane coupling agent, and its preparation method is:

[0077] Take 15 mg of fluorescein isothiocyanate (fluoresceinisothiocyanate, FITC) and 100 microliters of γ-aminopropyltriethoxysilane, add 5 milliliters of absolute ethanol, and stir for 48 hours in a sealed container under dark room conditions to obtain isothiocyanate Fluorescein thiocyanate modified silane coupling agent (FITC-APTES).

[0078] Steps 1 and 2 are the same as Steps 1 and 2 of Embodiment 1.

[0079] Step 3, take 20 mg of the magnetic mesoporous silica nanoparticles prepared in Step 2 of Example 1, disperse them in 6 ml of absolute ethanol, add 1 ml of FITC-APTES silane coupling agent, and stir in a sealed contai...

Embodiment 3

[0094] This example provides a method for preparing a γ-aminopropyltriethoxysilane-modified nano-drug with magnetic hyperthermia-chemotherapy synergistic effect, which is responsible for the anticancer drug DOX.

[0095] Steps 1, 2, and 3 are the same as in Example 1.

[0096] Step 4, take 150 mg of the nano-drug carrier prepared in step 3 of Example 1, and disperse it in 24 ml of PBS buffer solution of doxorubicin drug (0.5 mg / ml doxorubicin concentration), and shake the above mixed solution in the dark Shake at room temperature for 24 hours, then centrifuge, wash with PBS buffer solution, and finally obtain nano drug carrier for storing doxorubicin drug.

[0097] Step 5, take 200 microliters of DNA molecules with a chain length of 20 nucleotides and a carboxyl group modified at the 5' end (the DNA concentration is 100 micromoles), and add 35.6 microliters of EDC solution (the concentration of the EDC solution is 2.8 millimoles) After activating the carboxyl group for 10 min...

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Abstract

The invention provides a nano-drug carrier with a synergistic action of magnetic hyperthermia-chemotherapy. The nano-drug carrier is used for carrying nano-drug particles, and is characterized by comprising a mesoporous shell layer, a kernel and a deoxyribonucleic acid (DNA) molecular layer, wherein the mesoporous shell layer is formed by mesoporous silica particles; a plurality of mesoporous ducts are formed in the mesoporous shell layer; the mesoporous ducts form mesoporous at the surface of the mesoporous shell layer; the kernel is embedded in the internal part of the mesoporous shell layer and is formed by ferroferric oxide (Fe4O3) nano-particles; the DNA molecular layer is formed by DNA molecules encapsulated on the mesoporous; the mesoporous ducts penetrate the mesoporous shell layer for carrying the nano-drug particles; the mesoporous ducts are distributed in a radial pattern from the kernel to the mesoporous shell layer; the DNA molecules are nucleotide chains 20 to 40 in length. The invention also provides a nano-drug, carrying drugs, with the synergistic action of magnetic hyperthermia-chemotherapy. According to the nano-drug, the mesoporous ducts are encapsulated by using DNA molecules, so that the probability that anti-cancer drugs are leaked accidentally during a drug transportation process is reduced.

Description

technical field [0001] The invention relates to the fields of medicine and medicinal chemistry, in particular to a nano-medicine with magnetic hyperthermia-chemotherapy synergy and a corresponding preparation method. Background technique [0002] At present, cancer is still one of the major diseases threatening human life and health, and the incidence of cancer worldwide is on the rise. The treatment of cancer has always been a difficult point in research at home and abroad. The methods of clinical treatment of cancer include surgery, radiotherapy, hyperthermia and drug chemotherapy. Drug chemotherapy is a common method in cancer treatment, but many chemotherapeutic drugs cannot be transported in the body, are easily degraded and cleared, have poor drug selectivity, are difficult to specifically reach cancer cells, and most chemotherapeutic drugs are very harmful to normal cells. Great lethal effect. Therefore, the use of nano drug carriers for storage and delivery of anti...

Claims

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Application Information

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IPC IPC(8): A61K47/48A61K47/02A61K41/00A61K45/00A61P35/00
CPCA61K47/02A61K41/0052A61K45/00
Inventor 朱钰方徐怡
Owner UNIV OF SHANGHAI FOR SCI & TECH
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