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Method for efficiently preparing cycloastragenol

A high-efficiency technology for cycloastragenol, which is applied in the field of high-efficiency preparation of cycloastragenol, can solve the problems of low conversion efficiency of cycloastragenol, and achieve the effects of reduced production cost, low temperature and high conversion rate

Inactive Publication Date: 2016-05-11
安徽本森堂生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a method for efficiently preparing cycloastragenol to solve the above problems in order to solve the defect of low conversion efficiency of cycloastragenol in the prior art

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A method for efficiently preparing cycloastragenol is:

[0025] (1) Fermentation: put 100 grams of 100% astragalus total saponins into a fermenter, inject buffer solution containing 5 grams of compound enzyme until the sample is completely submerged, and carry out catalytic fermentation; the β-glucose in the mixed enzyme preparation used in the enzymatic hydrolysis glycosidase 60%; β-xylosidase 20%; naringinase 20%; the enzymolysis time is 6 hours, the temperature is 35°C, and the pH is 4.0.

[0026] (2) Alkali adjustment: After the catalysis, the fermentation broth obtained in step (1) was adjusted to pH 8.0 with sodium hydroxide, and left to stand for 1 hour.

[0027] (3) Countercurrent extraction: pump the fermented broth and extractant dichloromethane obtained in step (2) into a countercurrent extraction tower for extraction. The ratio of the flow rate of the fermented broth to the extractant is 1:2, and the extraction temperature is 20°C.

[0028] (4) Concentratin...

Embodiment 2

[0031] (1) Fermentation: Put 100 grams of 60% total astragalosides into a fermenter, inject buffer solution containing 0.5 grams of compound enzyme until the sample is completely submerged, and carry out catalytic fermentation; the β-glucose in the mixed enzyme preparation used in the enzymatic hydrolysis glycosidase 40%; β-xylosidase 10%; naringinase 50%; the enzymolysis time is 4h, the temperature is 60°C, and the pH is 6.0;

[0032] (2) Alkali adjustment: After the catalysis, the fermentation broth obtained in step (1) was adjusted to pH 10.0 with potassium hydroxide, and left to stand for 1 hour.

[0033] (3) Countercurrent extraction: pump the fermented broth and extractant methylene chloride obtained in step (2) into a countercurrent extraction tower for extraction, the ratio of the flow rate of the fermented broth to the extractant is 1:6, and the extraction temperature is 30°C

[0034] (4) Concentrate and dry the mixture of cycloastragenol and extractant obtained in st...

Embodiment 3

[0037] A method for efficiently preparing cycloastragenol is:

[0038] (1) Fermentation: Put 1 kg of 80% total astragalosides into a fermenter, inject a buffer solution containing 25 grams of compound enzyme until the sample is completely submerged, and carry out catalytic fermentation; the β-glucose in the mixed enzyme preparation used in the enzymatic hydrolysis glycosidase 50%; β-xylosidase 20%; naringinase 30%; the enzymolysis time is 5h, the temperature is 50°C, and the pH is 5.0;

[0039] (2) Alkali adjustment: After the catalysis, the fermentation broth obtained in step (1) was adjusted to pH 9.0 with sodium hydroxide, and left to stand for 1 hour.

[0040] (3) Countercurrent extraction: pump the fermented broth and extractant methylene chloride obtained in step (2) into a countercurrent extraction tower for extraction, the ratio of the flow rate of the fermented broth to the extractant is 1:4, and the extraction temperature is 25°C

[0041] (4) Concentrating and dryin...

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Abstract

The invention provides a method for efficiently preparing cycloastragenol. The method sequentially comprises the following steps of A, fermenting: filling total saponins of radix astragali seu hedysari into a fermentation tank, injecting a buffer solution containing compound enzyme into the fermentation tank until a sample is completely immersed, stirring and then performing catalytic fermentation; B, regulating alkali: after catalysis is ended, regulating fermentation liquid obtained in the step A into an alkali solution, and standing for one hour; C, performing counter-current extraction: pumping the fermentation liquid obtained in the step B and an extraction agent into a counter-current extraction tower for extracting; D, performing vacuum concentration on a mixed solution, which is obtained by extracting, of the cycloastragenol and the extraction agent. The method provided by the invention has the beneficial effects that firstly, a compound enzyme fermentation technology and a continuous counter-current extraction technology are combined for extracting and purifying to obtain high-purity cycloastragenol, and high conversion rate and higher purity of products are realized; secondly, the finishing time of the whole process does not exceed eight hours; the temperature is low in the operation process, so that biological activity of the cycloastragenol is guaranteed; the production efficiency is improved, energy consumption is reduced, and the production cost is reduced by 50 percent or above 50 percent.

Description

technical field [0001] The invention relates to the field of medicine and chemical industry, in particular to a method for efficiently preparing cycloastragenol. Background technique [0002] Cycloastragenol (CAG) is a tetracyclic triterpenoid cycloartane compound derived from the traditional Chinese medicine Astragalus, with a molecular formula of C30H50O5 and a relative molecular weight of 490.71. Cycloastragenol can promote the proliferation of lymphocytes through the activation of telomerase, thereby delaying the aging of cells. At present, CAG is used as an effective wound repair medicine and the only raw material of health care products that can activate telomerase to play an anti-aging effect on the market. It plays an irreplaceable role in the anti-aging product market and has broad market prospects. [0003] There are nearly 20 saponins isolated and identified in Astragalus membranaceus, most of which are cycloartane-type tetracyclic triterpene saponins. However,...

Claims

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Application Information

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IPC IPC(8): C07J53/00C12P33/20
CPCC07J53/004C12P33/20
Inventor 王亚储浩侠刘玉周伟王佳丽周雨晴
Owner 安徽本森堂生物科技有限公司
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