Biomarker for detecting esophagus cancer

A biomarker, esophageal cancer technology, applied in the field of tumor markers

Active Publication Date: 2016-05-11
CANCER INST & HOSPITAL CHINESE ACADEMY OF MEDICAL SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the accuracy of MALDI-TOF is as high as 0.1%-0.01%, which is much higher than that of sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and high-performance liqui...

Method used

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  • Biomarker for detecting esophagus cancer
  • Biomarker for detecting esophagus cancer
  • Biomarker for detecting esophagus cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0080] Example 1 Mass Spectrometry Detection of Esophageal Cancer Serum Polypeptides and Establishment of Esophageal Cancer Screening Model

[0081] 1. Samples and instruments:

[0082] Serum samples were selected from 397 cases, of which 201 were from patients with esophageal cancer and the other 196 were from healthy people. All patients with esophageal cancer were diagnosed as esophageal squamous cell carcinoma by postoperative pathological reports. All serum samples were taken in the morning on an empty stomach, and the serum was separated and stored in a -80°C low-temperature refrigerator.

[0083] Matrix-assisted laser desorption time-of-flight mass spectrometry Clin-TOF (Yixing Industry (Beijing) Technology Co., Ltd.) and the experimental WCX magnetic bead kit (SPE-C magnetic bead kit, see Chinese patent ZL2008101879684) were developed by Yixing Industry Company . The analysis processing of data can adopt the software commonly used in this field, for example the Clinp...

Embodiment 2

[0119] Example 2 Blind selection test of esophageal cancer screening model

[0120] 198 of 397 serum samples (201 from patients with esophageal cancer and 196 from healthy people) were randomly selected as training samples for the establishment of the model, 100 of which were from patients with esophageal cancer, including 24 cases of early esophageal cancer ( TNM stage I), and the other 98 cases were from healthy people; patients with esophageal cancer were confirmed by postoperative pathological reports. All serum samples were taken in the morning on an empty stomach, and the serum was separated and stored in a -80°C low-temperature refrigerator.

[0121] Another 199 remaining samples were selected as verification samples for blind selection testing, among which 101 were known to be from patients with esophageal cancer, including 19 cases of early-stage esophageal cancer (TNM stage I), and the other 98 were from healthy people. The processing method is the same as above.

...

Embodiment 3

[0132] Example 3 TSP1 protein can be used to distinguish esophageal squamous cell carcinoma and normal population at the tissue level

[0133] The identified thrombospondin 1 (TSP1) polypeptide fragment (2950.6m / z) was highly expressed in the serum of patients with esophageal squamous cell carcinoma (ESCC). In order to detect the expression of TSP1 in tissues, we carried out Immunohistochemical detection.

[0134] Anti-TSP1 antibody (Cat. No. 18304-1-AP, ProteintechGroup Inc., IL, USA) was used to detect the tissue chip (Shanghai Xinchao Biotechnology Co., Ltd., lot No. -Squ172Sur-01) for staining. The enhanced one-step Polymer detection system (Beijing Zhongshan Jinqiao Biotechnology Co., Ltd., Cat. No. PV-6001) was used for staining. The tissue chip was dewaxed with xylene, hydrated with graded ethanol, and immersed in 30 mL / L H 2 o 2 10 min to block endogenous peroxidase activity. After the slices were washed with PBS, they were placed in a 0.01 mol / L, pH 6.0 citrate bu...

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Abstract

The invention provides a biomarker compound for early diagnosing esophagus cancer and specifically provides a characteristic polypeptide compound capable of passing mass spectrum detection. The compound comprises three polypeptides at the mass-to-charge ratio of 1925.5m/z, 2950.6m/z and 5900.0m/z. When the 2950.6m/z and 5900.0m/z polypeptides are subjected to up-regulation expression and 1925.5m/z polypeptide is subjected to down-regulation expression, the subject is confirmed as an esophagus cancer patient or a potential esophagus cancer patient. The biomarker provided by the invention can be used for early diagnosing and screening the esophagus cancer; the method is simple, easy in operation and high in accuracy; a new method and thought for early diagnosing and screening the esophagus cancer are supplied; and meanwhile, the invention also discovers that thrombin sensitive protein 1 (TSP1) can be singly taken as a biomarker used for diagnosing the esophagus cancer or forecasting the prognosis of the esophagus cancer in serum or tissue.

Description

technical field [0001] The present invention relates to the field of tumor markers, and relates to biomarkers for early diagnosis of esophageal cancer. Specifically, the present invention provides a characteristic polypeptide combination that can be detected by mass spectrometry as a biomarker for early diagnosis of esophageal cancer. Background technique [0002] Esophageal cancer is the eighth most common cancer worldwide and the sixth leading cause of cancer-related death. Although medical research institutions in various countries have made progress in the diagnosis and treatment of esophageal cancer, its five-year survival rate is only 15% on average, and there is still a lack of reliable diagnostic methods, early detection methods and effective individualized treatment programs. And the prognosis is poor. According to research reports, if the tumor in the early stage is successfully removed, the 5-year overall survival rate of patients with esophageal squamous cell ca...

Claims

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Application Information

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IPC IPC(8): G01N33/574
CPCG01N33/57407G01N33/57484
Inventor 赵晓航孙玉琳贾坤周兰萍马庆伟王凤林燕
Owner CANCER INST & HOSPITAL CHINESE ACADEMY OF MEDICAL SCI
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