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Production method of selenium-rich ganoderma lucidum mycelium raw material

A technology of ganoderma mycelium and production method, which is applied in the field of selenium-enriched ganoderma lucidum mycelium raw materials, can solve the problems of high labor intensity, unstable quality and output, long cycle of ganoderma lucidum, etc., and achieve the effect of reducing production costs and protecting public health

Inactive Publication Date: 2016-05-18
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, Ganoderma lucidum is mainly obtained by artificial cultivation, using sporocarp or spore powder as medicine, but artificial cultivation of Ganoderma lucidum has long cycle, low production efficiency, high labor intensity, and is subject to season and environment restrictions. It is vulnerable to pests and diseases, and its quality and output are unstable

Method used

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  • Production method of selenium-rich ganoderma lucidum mycelium raw material
  • Production method of selenium-rich ganoderma lucidum mycelium raw material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0073] Ganoderma lucidum CCTCCM2015066 was used as the strain of Ganoderma lucidum. The amount of rice bran used is 8.0g / 100mL medium, the amount of bran used is 8.0g / 100mL medium, and KH 2 PO 4 0.36g / 100mL, MgSO 4‘ 7H 2O0.36g / 100mL, cysteine ​​hydrochloride 50mg / 100mL, sodium copper chlorophyllin 10mg / 100mL, pH natural, add water to the required volume, sterilize at 121°C for 30min, use it as a medium for fermentation, shake bottle The sample volume was 40%, the inoculum volume was 10%, and the culture temperature was 28 o C, rotating speed 180r / min, add 2.68mg / 100mL sterilized sodium selenite solution after 2 days of fermentation, the total fermentation time is 7 days. The following steps (3) to (9) in the technical solution described in the foregoing summary of the invention are implemented. The results are: mycelium dry weight 5.2g / 100mL medium, mycelium polysaccharide 256.8mg / 100mL medium, exopolysaccharide 573.9mg / 100mL medium, the content of selenium in mycelium is...

Embodiment 2

[0075] Ganoderma lucidum CCTCCM2015066 was used as the strain of Ganoderma lucidum. The amount of rice bran used is 5.0g / 100mL medium, the amount of bran used is 5.0g / 100mL medium, and KH 2 PO 4 0.015g / 100mL, MgSO 4‘ 7H 2 O0.015g / 100mL, cysteine ​​hydrochloride 20mg / 100mL, sodium copper chlorophyllin 5mg / 100mL, pH natural, add water to the required volume, sterilize at 121°C for 30min, use as medium for fermentation, shake bottle The sample volume was 40%, the inoculum volume was 8%, and the culture temperature was 24 o C, rotating speed 150r / min, add 0.45mg / 100mL sterilized sodium selenite solution after fermentation 2d, total fermentation time is 5d. The following steps (3) to (9) in the technical solution described in the foregoing summary of the invention are implemented. The results are: mycelium dry weight 3.3g / 100mL medium, mycelium polysaccharide 157.5mg / 100mL medium, exopolysaccharide 340.8mg / 100mL medium, the content of selenium in mycelia is 22.57μg / g dry mycel...

Embodiment 3

[0077] Ganoderma lucidum CCTCCM2015066 was used as the strain of Ganoderma lucidum. The amount of rice bran used is 6.5g / 100mL medium, the amount of bran used is 6.5g / 100mL medium, and KH 2 PO 4 0.18g / 100mL, MgSO 4‘ 7H 2 O0.18g / 100mL, cysteine ​​hydrochloride 35mg / 100mL, sodium copper chlorophyllin 7.5mg / 100mL, pH natural, add water to the required volume, sterilize at 121°C for 30min, use it as a medium for fermentation, shake The sample volume in the bottle is 40%, the inoculum volume is 9%, and the culture temperature is 26 o C, rotating speed 165r / min, add 1.34mg / 100mL sterilized sodium selenite solution after 2 days of fermentation, the total fermentation time is 9 days. The following steps (3) to (9) in the technical solution described in the foregoing summary of the invention are implemented. The results are: the dry weight of mycelia is 4.3g / 100mL medium, the mycelium polysaccharide is 209.6mg / 100mL medium, the exopolysaccharide is 462.7mg / 100mL medium, the conten...

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Abstract

The invention discloses a production method of a selenium-rich ganoderma lucidum mycelium raw material and relates to the technical field of food microorganism application. According to the production method, a new ganoderma lucidum strain obtained through ultraviolet mutagenesis is adopted to ferment new liquid-state rice bran and wheat bran complete culture media with addition of selenium, cysteine hydrochloride and sodium copper chlorophyllin, rice bran and wheat bran are efficiently converted into the selenium-rich ganoderma lucidum mycelium raw material and more crude extracellular selenium polysaccharide, the production cost is reduced, the yield is increased, and the selenium-rich ganoderma lucidum mycelium raw material with better quality is prepared; the crude extracellular selenium polysaccharide is reserved as a raw material for further deep processing. The low-value rice bran and wheat bran are converted into the selenium-rich ganoderma lucidum mycelium raw material with multiple healthcare functions, and the production method of the selenium-rich ganoderma lucidum mycelium raw material has beneficial social and economic effects in the aspects of production cost reduction, efficient and reasonable low-grade resource utilization and public health protection, thereby having practicability.

Description

technical field [0001] The invention relates to the technical field of food microorganism application, in particular to a method for producing selenium-enriched Ganoderma lucidum mycelia raw material by using a new strain of Ganoderma lucidum obtained by mutagenesis. In the method, the new strain is added with cysteine ​​hydrochloride and copper chlorophyll. Fermentation is carried out in the liquid rice bran whole material new medium of sodium salt and sodium selenite to obtain the selenium-enriched ganoderma mycelia raw material. Background technique [0002] Medicinal and edible fungi have a long history of use in China and a wide range of users. Modern science reveals that the mycelia, fruiting bodies or spores of commonly used medicinal and edible fungi such as Ganoderma lucidum, Hericium erinaceus, Cordyceps sinensis, and Grifola frondosa can produce amino acids, proteins, vitamins, polysaccharides, nucleosides, Various active ingredients such as flavonoids and antibi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12P19/04C12R1/645
CPCC12N1/14C12P19/04
Inventor 刘伟民李婷婷王冲之任晓峰朱文静王雪梅伍娟程宇
Owner JIANGSU UNIV
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