Method for producing heavy metal absorbent through microbiological treatment on lignite and related compound microbial agent

A technology of composite microbial agent and heavy metal, applied in the field of resource utilization of lignite, can solve few problems and achieve the effect of improving capacity

Inactive Publication Date: 2016-05-18
江苏加德绿色能源有限公司
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  • Abstract
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  • Application Information

AI Technical Summary

Problems solved by technology

The above-mentioned patents or documents all use physical and chemical methods to process lignite to produce adsorbents, which involve strong acid and strong alkali treatment. While obtaining products, they also cause a certain pressure on the environment. Microbiological methods are rarely used to process lignite to produce heavy metal adsorbents.

Method used

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  • Method for producing heavy metal absorbent through microbiological treatment on lignite and related compound microbial agent

Examples

Experimental program
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Effect test

Embodiment 1

[0024] The preparation of embodiment 1 fermented liquid

[0025] In this example, Beauveria bassiana was purchased from ATCC, numbered ATCC7159; Bacillus subtilis was purchased from ACCC, numbered ACCC19374; Candida tropicalis was purchased from ATCC, numbered ATCC20326.

[0026] (1) Activate the above-mentioned Beauveria bassiana (ATCC7159), Bacillus subtilis (ATCC11775), and Candida tropicalis (ATCC20326) into their respective slant media for preservation, and culture them at 26-37°C for 16-72 hours.

[0027] Preservation of three kinds of microorganisms uses the slant culture medium formula as follows:

[0028] The medium used for Beauveria bassiana: 200 grams of potato, 20 grams of glucose, 10 grams of peptone, 15 grams of agar, 1000 ml of tap water, natural pH;

[0029] Medium for Bacillus subtilis: 10 grams of peptone, 5 grams of glucose, 3 grams of beef extract, 5 grams of sodium chloride, 15 grams of agar, 1000 ml of tap water, pH7.2±0.2;

[0030] The medium used for...

Embodiment 2

[0035] Example 2 Adsorption of copper-containing test solution after processing lignite with composite microbial bacterial agent

[0036] 1. Select 8kg of lignite coal powder (moisture content 20.0%) that has been crushed through a 80 mesh sieve, divide it into 4 parts on average, add in 4 20L reactors respectively, add 150g of soybean meal hydrolyzate (Shandong Yangcheng Biotechnology Co., Ltd.) and A total of 50g of urea, 100g of glucose, adding water to adjust the moisture content of the material to 90%, adjust the pH to 7.0, and the total volume is 16L;

[0037] 2, three kinds of microbial fermented liquids prepared in embodiment 1 are combined into composite microbial inoculum by following parts by weight: 20 parts by weight of Beauveria bassiana fermented liquid, 9 parts by weight of Bacillus subtilis fermented liquid, 8 parts by weight of Candida tropicalis fermented liquid parts by weight;

[0038] 3. Add 48ml each of the following microbial fermentation broth or micr...

Embodiment 3

[0044] Example 3 Adsorption of lead-containing test solution after processing lignite with composite microbial bacterial agent

[0045] 1. Select 12kg of lignite coal powder (moisture content 28.6%) that has been crushed through a 80-mesh sieve, divide it into 4 parts on average, add them to 4 20L reactors, then add 150g of peptone and 210g of glucose, and add water to adjust the moisture content of the material to 88%. , adjust the pH to 6.8, and the total volume is 17.9L;

[0046] 2. Prepare three kinds of microbial fermented liquids according to the method of Example 1, and mix uniformly by the following parts by weight to become composite microbial inoculum I: 19 parts by weight of Beauveria bassiana fermented liquid, 10 parts by weight of Bacillus subtilis fermented liquid, tropical pseudo 7 parts by weight of fermented liquid of Trichosomyces; mix uniformly according to the following parts by weight to form composite microbial agent II: 4 parts by weight of Beauveria bas...

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Abstract

The invention discloses a method for producing a heavy metal absorbent through microbiological treatment on lignite and a related compound microbial agent, relating to a method for preparing the heavy metal absorbent through the microbiological treatment. The compound microbial agent is composed of fermentation liquor of beauveria bassiana, bacillus subtilis and candida tropicalis. The compound microbial agent can react with various groups in the lignite, so that the capacity of the lignite for adsorbing heavy metal ions is further promoted, and the synergy of the three microbial strains is superior to the effect of any one microbial strain and the effect of any two microbial strains.

Description

technical field [0001] The invention belongs to the field of resource utilization of lignite, and relates to a method for microbially treating lignite to produce a heavy metal adsorbent and a composite microbial bacterial agent involved. Background technique [0002] Lignite is the initial product of peat deposition and dehydration and compaction into organic biorock. The degree of aromatic nuclei condensation of humic acid in lignite is higher, the oxygen-containing functional groups are reduced, and the side chains are shorter and less in number. The phenolic hydroxyl group, carboxyl group, ether bond and carbonyl group contained in the polycyclic aromatic structure of lignite polycondensation are easy to dissociate, have hydrophilic properties, and can interact with metal ions through complexation or ion exchange. It is a good metal ion adsorption material . Regarding the use of lignite as an adsorbent to treat metal ions such as Cr in water 6+ 、Cu 2+ , Pb 2+ etc. Th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/16C12N1/14B01J20/24C02F1/28C12R1/125C12R1/645C12R1/74C02F101/20
Inventor 李登奎
Owner 江苏加德绿色能源有限公司
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