Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A kind of trichoderma spore preparation and preparation method thereof

A technology of Trichoderma and spores, applied in the field of bioengineering, to achieve the effects of reducing production costs, promoting plant growth, and improving soil fertilizer supply capacity

Active Publication Date: 2018-10-23
CHAMBROAD CHEM IND RES INST CO LTD
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

With the increase of chitosan oligosaccharide production, the amount of residue also gradually increases, which brings pressure on the later solid waste treatment

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of trichoderma spore preparation and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] (1) Activation of Trichoderma strains: transfer the Trichoderma viride strains stored on the PDA medium at 4°C to 25°C for activation for 6 hours;

[0059] (2) Trichoderma liquid seed preparation:

[0060] The composition of the liquid seed medium is by weight (w / w): 3% sucrose, 0.8% yeast extract powder, 0.08% magnesium sulfate, 0.04% potassium dihydrogen phosphate, and add water to 1000mL;

[0061] Sterilize the Erlenmeyer flask containing the above culture medium in an autoclave at 121°C for 30 minutes, take it out, and cool it to room temperature;

[0062] On the aseptic operating table, use 10mL of sterile water to make the activated test tube Trichoderma slant strain into a bacterial suspension, rinse it into a triangular flask containing liquid seed medium, and the inoculum amount is 1 test tube strain Inoculate 1 triangular flask;

[0063] Liquid seed culture conditions: pH value is natural, culture temperature is 28°C, shaker speed is 180r / min, culture time i...

Embodiment 2

[0081] (1) Activation of Trichoderma strains: transfer the Trichoderma konii strains stored on the PDA medium at 4°C to 22°C for activation for 4 hours;

[0082] (2) Trichoderma liquid seed preparation:

[0083] The composition of the liquid seed medium is by weight (w / w): 4% sucrose, 1% yeast extract powder, 0.1% magnesium sulfate, 0.02% potassium dihydrogen phosphate, and add water to 1000mL;

[0084] Sterilize the Erlenmeyer flask containing the above culture medium in an autoclave at 121°C for 30 minutes, take it out, and cool it to room temperature;

[0085] On the aseptic operating table, use 10 mL of sterile water to prepare the activated test tube slant bacteria into a bacterial suspension, rinse it into a triangular flask containing liquid seed medium, and inoculate 1 test tube with 1 a triangular flask;

[0086] Liquid seed culture conditions: pH value is natural, culture temperature is 26°C, shaker speed is 140r / min, culture time is 48h, and Trichoderma liquid see...

Embodiment 3

[0102] (1) Activation of Trichoderma strains: transfer Trichoderma harzianum strains stored on the PDA medium at 4°C to 23°C for 8 hours;

[0103] (2) Trichoderma liquid seed preparation:

[0104] The composition of the liquid seed medium is by weight (w / w): 4% sucrose, 0.6% yeast extract powder, 0.04% magnesium sulfate, 0.03% potassium dihydrogen phosphate, and add water to 1000mL;

[0105] Sterilize the Erlenmeyer flask containing the above culture medium in an autoclave at 121°C for 30 minutes, take it out, and cool it to room temperature;

[0106] On the aseptic operating table, use 10 mL of sterile water to prepare the activated test tube slant bacteria into a bacterial suspension, rinse it into a triangular flask containing liquid seed medium, and inoculate 1 test tube with 1 a triangular flask;

[0107] Liquid seed cultivation conditions: natural pH value, cultivation temperature of 24°C, shaker rotation speed of 160r / min, cultivation time of 36h, to obtain Trichoderm...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the technical field of bioengineering, and particularly relates to a trichoderma spore preparation and a preparation method thereof. The trichoderma spore preparation comprises the effective ingredients of chitosan enzymatic hydrolysis residues and trichoderma spores. The growth promoting and bacterium preventing functions of chitosan and the bacterium preventing function of trichoderma are combined, the biocontrol activity of the produced trichoderma spore preparation is improved by 30% or above, plant growth is obviously promoted, the yield of crops is increased by 15% or above, soil can further be improved, the fertilizer supply capacity of the soil can be improved, the growth of beneficial microorganisms in soil can be promoted, the colonization capacity of trichoderma in the soil is enhanced, and therefore the biocontrol of trichoderma is improved. The chitosan enzymatic hydrolysis residues generated in the production process of chitosan are adopted as raw materials, waste is really turned into wealth, the production cost is reduced, and meanwhile the processing problem of the chitosan enzymatic hydrolysis residues is solved. The process is reasonable, low in cost and easy to implement, product toxicity is low, no residue exists, and good economic benefits, environmental benefits and social benefits are achieved.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and in particular relates to a Trichoderma spore preparation and a preparation method thereof. Background technique [0002] Trichoderma (Trichoderma spp.) belongs to the subphylum Deuteromycotina, Hyphophyceae, Synchospora, Cyclosporaceae, Myxosporum, widely exists in soil and air, and can survive in tropical to tundra zones It is the most studied plant biocontrol fungus so far. A large number of studies suggest that Trichoderma has multiple antagonistic mechanisms against plant pathogens, such as competition, reparasitism, enzyme lysis, antibiotic production, and induction of plant resistance. Most Trichoderma, such as Trichoderma viride, Trichoderma harzianum, Trichoderma korningen, Glioma viridans and other Trichoderma have broad-spectrum antagonistic effects, and the pathogenic fungi that can be antagonized include Rhizoctonia solani, Pythium, Phytophthora, Fusarium, white silkworm,...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A01N63/04A01N43/16A01N25/12A01N25/14A01P21/00A01P3/00C05G3/00
CPCA01N25/12A01N25/14A01N43/16A01N63/30C05G3/00A01N2300/00
Inventor 秦森姚刚杨传伦张心青王建平
Owner CHAMBROAD CHEM IND RES INST CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com