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Method for extracting lysozyme from egg white or whole egg juice

A technology of egg liquid and egg white, applied in the biological field, can solve the problems that are not conducive to co-separation and preparation of egg white, and achieve high purity and biological activity, cost reduction, and high biological activity.

Inactive Publication Date: 2016-05-25
YANTAI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above methods are all unfavorable for co-separation and preparation of other active proteins such as ovalbumin and ovotransferrin in egg white

Method used

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  • Method for extracting lysozyme from egg white or whole egg juice

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Effect test

Embodiment 1

[0034] Such as figure 1 As shown, the method for extracting lysozyme from egg white or whole egg liquid of the present embodiment comprises the following steps:

[0035] (1) Add 1 times the weight of water to egg white or whole egg liquid, mix evenly, filter with a filter cloth, remove the lipid components in the egg yolk, and obtain a dilution;

[0036] (2) The diluted solution obtained in step (1) is ultrafiltered with an ultrafiltration membrane with a molecular weight cut-off of 20000Da to obtain a thin solution and a concentrated solution, and the concentrated solution is dried to obtain a high-molecular protein, and the high-molecular protein is used for Preparation of ovalbumin and ovotransferrin;

[0037] (3) Adding the cation exchange resin that model is 001*7 to the weak liquid that step (2) obtains is adsorbed, and the weight ratio of described weak liquid and resin is 1:3, obtains the resin after adsorption;

[0038] (4) eluting the adsorbed resin obtained in ste...

Embodiment 2

[0042] Such as figure 1 As shown, the method for extracting lysozyme from egg white or whole egg liquid of the present embodiment comprises the following steps:

[0043] (1) Add 5 times the weight of water to egg white or whole egg liquid, mix evenly, filter with a filter cloth, remove the lipid components in the egg yolk, and obtain a dilution;

[0044] (2) The diluted solution obtained in step (1) is ultrafiltered with an ultrafiltration membrane with a molecular weight cut-off of 30000Da to obtain a thin solution and a concentrated solution, and the concentrated solution is dried to obtain a high-molecular protein, and the high-molecular protein is used for Preparation of ovalbumin, ovotransferrin, ovomucin and vitellin;

[0045] (3) in the weak liquid that step (2) obtains, add the cationic exchange resin that model is AmberliteIR-120 to adsorb, and the weight ratio of described weak liquid and resin is 1:5, obtains the resin after adsorption;

[0046] (4) eluting the ad...

Embodiment 3

[0050] Such as figure 1 As shown, the method for extracting lysozyme from egg white or whole egg liquid of the present embodiment comprises the following steps:

[0051] (1) Add 10 times the weight of water to egg white or whole egg liquid, mix evenly, filter with a filter cloth, remove the lipid components in the egg yolk, and obtain a dilution;

[0052](2) The diluted solution obtained in step (1) is ultrafiltered with an ultrafiltration membrane with a molecular weight cut-off of 15000Da to obtain a thin solution and a concentrated solution, and the concentrated solution is dried to obtain a high-molecular protein, and the high-molecular protein is used for Preparation of ovalbumin, ovotransferrin, ovomucin and vitellin;

[0053] (3) add the macroporous adsorption resin that model is D101 in the dilute liquid that step (2) obtains and adsorb, and the weight ratio of described dilute liquid and resin is 1:8, obtains the resin after adsorption;

[0054] (4) eluting the adso...

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Abstract

The invention discloses a method for extracting lysozyme from egg white or whole egg juice, and belongs to the technical field of biology. The method comprises the following steps: (1) adding 1-10-fold water to the egg white or whole egg juice, mixing evenly, and filtering out impurities to obtain a diluent; (2) carrying out ultrafiltration on the diluent with an ultrafiltration membrane to obtain a thin liquid and a thick liquid; (3) adding resin to the thin liquid for adsorption, wherein the weight ratio of the thin liquid to the resin is (1:3) to (1:8), and the adsorbed resin is obtained; (4) eluting the adsorbed resin with a 0.001-0.5mol / L NaCl water solution to obtain an eluant; (5) desalting the eluant with the ultrafiltration membrane to obtain ultrafiltrate; and (6) drying the ultrafiltrate to obtain the lysozyme. The method is simple and fast in process, mild in condition, and free of complicated equipment; high-purity and high-bioactivity lysozyme can be obtained; and the defects of an existing lysozyme preparation technology can be overcome.

Description

technical field [0001] The invention relates to a method for extracting lysozyme from egg white or whole egg liquid, and belongs to the field of biotechnology. Background technique [0002] Lysozyme is a basic protein, which is widely distributed in animals, plants and microorganisms, and is expressed in mammalian plasma, trachea, intestinal tract, stomach, kidney and liver tissue cells. Lysozyme can be used as a potential substitute for antibiotics in the fields of food, bioengineering, medical treatment, and livestock and poultry feed due to its antibacterial properties, non-toxicity, and side effects. [0003] At present, the preparation methods of lysozyme mainly include direct crystallization, ion exchange, ultrafiltration, polyelectrolyte precipitation, affinity chromatography and so on. The operation of the direct crystallization method is simple, but the production cycle is long, the yield is low, the purity and activity are also low, and it is not suitable for indu...

Claims

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Application Information

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IPC IPC(8): C12N9/36
CPCC12N9/2462C12Y302/01017
Inventor 姜爱莉刘雪梅单守水石礼娟
Owner YANTAI UNIV
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