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Method for preparing biological butyl alcohol with marine algae as raw materials

A technology of biological butanol and a production method, applied in the field of bioengineering, can solve problems such as affecting the decomposition and utilization of polysaccharides, and achieve the effects of improving high-efficiency output and promoting industrialization development.

Inactive Publication Date: 2016-05-25
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

First, the algae cell wall barrier affects the decomposition and utilization of intracellular polysaccharides, and the brown algae polyphenols in the seaweed treatment liquid have an inhibitory effect on the growth of bacteria, etc.

Method used

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  • Method for preparing biological butyl alcohol with marine algae as raw materials

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Seaweed pretreatment and saccharification: After pretreatment of seaweed homogenate with a concentration of 180g / L, suction filtration, centrifugation and solid-liquid separation, the solid part was taken for saccharification, the amount of cellulase added was 15FPU / g substrate, and the enzymatic hydrolysis treatment was carried out for 3 days. The enzymatic hydrolysis solution is vacuum-distilled to half of the original volume after suction filtration and centrifugation for solid-liquid separation;

[0043] Fermentation medium preparation: After the seaweed hydrolyzate is cooled to room temperature, add this as the main raw material, add ammonium sulfate until the ammonium sulfate in the hydrolyzate is 10.8g / L, add potassium dihydrogen phosphate until the content of potassium dihydrogen phosphate in the hydrolyzate is 5.0g / L, and 1.1g / L, add sodium acetate until the sodium acetate content in the hydrolyzate is 3.0g / L, add potassium dihydrogen phosphate until the conten...

Embodiment 2

[0052] Seaweed pretreatment and saccharification: After pretreatment, the laver homogenate with a concentration of 90g / L was pretreated, suction filtered, centrifuged to separate the solid from the liquid, and the solid part was taken for saccharification. The amount of cellulase added was 15FPU / g substrate, and the enzymatic hydrolysis treatment was carried out for 3 days. The enzymatic hydrolysis solution is vacuum-distilled to half of the original volume after suction filtration and centrifugation for solid-liquid separation;

[0053] Preparation of fermentation medium: After the seaweed hydrolyzate is cooled to room temperature, add this as the main raw material, add ammonium sulfate until the ammonium sulfate in the hydrolyzate is 5.4g / L, add potassium dihydrogen phosphate until the content of potassium dihydrogen phosphate in the hydrolyzate is 5.0g / L, 1.1g / L Add sodium acetate until the sodium acetate content in the hydrolyzate is 3.0g / L, add potassium dihydrogen phosph...

Embodiment 3

[0057] Seaweed pretreatment and saccharification: pretreat the undaria pinnata homogenate with a concentration of 120g / L, suction filter, centrifuge to separate the solid from the liquid, take the solid part for saccharification, add 15 FPU / g substrate of cellulase, and enzymatically hydrolyze for 3 days , the enzymolyzed solution was filtered and centrifuged for solid-liquid separation, and the liquid part was distilled under reduced pressure to half of the original volume;

[0058] Fermentation medium preparation: After the seaweed hydrolyzate is cooled to room temperature, add this as the main raw material, add ammonium sulfate until the ammonium sulfate in the hydrolyzate is 5.4g / L, add potassium dihydrogen phosphate until the content of potassium dihydrogen phosphate in the hydrolyzate is 5.0g / L, 1.1g / L Add sodium acetate until the sodium acetate content in the hydrolyzate is 3.0g / L, add potassium dihydrogen phosphate until the content of potassium dihydrogen phosphate in...

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Abstract

The invention belongs to the technical field of bioengineering and particularly relates to a method for preparing biological butyl alcohol with marine algae as raw materials. The method comprises the steps that marine algae are pretreated and saccharified to obtain marine alga hydrolysate, and the marine alga hydrolysate serves as the raw materials to prepare biological butyl alcohol. According to the method, marine algae serve as the raw materials and are prepared and then used for preparing a fermentation medium, and continuous anaerobic fermentation is carried out for 72-120 h at 35-40 DEG C to prepare biological butyl alcohol through inoculated fermentation. Compared with a traditional method, the method has the advantages that land for people is not occupied, and grain in the land is not influenced. In the method, cellulase is used for carrying out saccharifying pretreatment on the marine algae, the cell wall screen of the algae is broken through, and polysaccharide in cells of the marine algae is decomposed and utilized. In addition, by means of the method, the inhibiting effect of brown algae polyphenol and the like in a marine algae treatment solution on growth of thalli is eliminated, the high-efficiency yield of biological butyl alcohol is achieved and improved, and great significance is achieved on promoting the industrialization development of production of marine algae biological butyl alcohol, protecting the environment and guaranteeing grain safety.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and in particular relates to a method for producing bio-butanol by using marine algae as a raw material, obtaining a seaweed hydrolyzate through pretreatment and saccharifying the seaweed. Background technique [0002] With the gradual depletion of fossil energy, especially oil. The development of new alternative energy sources mainly based on bioenergy is imperative. Butanol is an important platform chemical, mainly used in the manufacture of plasticizers or as solvents, extractants, etc.; in recent years, researchers have found that the calorific value and octane number of butanol are comparable to gasoline; its oxygen content It is similar to methyl tert-butyl ether commonly used in gasoline; it does not corrode pipes, is not easy to absorb water, and is convenient for pipeline transportation; it has low vapor pressure, high safety, and can be mixed with gasoline in any ratio. Therefo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/16C12R1/145
CPCC12P7/16Y02E50/10
Inventor 叶乃好张晓雯徐东范晓
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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