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Method for modifying magnetic bentonite by biosurfactant and application of modified magnetic bentonite

A biological surface, active agent technology, applied in the methods of using microorganisms, chemical instruments and methods, radioactive purification, etc., can solve the problems of difficult and rapid separation and recovery, affecting recycling, etc., to achieve strong chemical stability, ensure contact, and reaction. mild conditions

Active Publication Date: 2016-06-01
NANHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, after the adsorbent with smaller particle size absorbs uranium ions, it will be suspended in the uranium-containing wastewater for a longer time, which makes it difficult to separate and recover quickly, thus affecting its recycling.

Method used

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  • Method for modifying magnetic bentonite by biosurfactant and application of modified magnetic bentonite
  • Method for modifying magnetic bentonite by biosurfactant and application of modified magnetic bentonite

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] The first step, the enrichment culture and domestication of strains

[0036] Air-dried 100g of rice straw and 100g of chaff were crushed and sieved to obtain powdery biomass, and 500g of farmland soil samples were collected from field farmland. Use powdered biomass as the initial compost material, bury it in the farmland soil, and add 10g of air-dried and crushed bark as a filler to improve the porosity of the compost. After culturing at 4-5°C for 7 days, store them until use.

[0037] Take 10g of compost samples and place them in a 250mL Erlenmeyer flask filled with 90mL of sterile water, vibrate for 25min, then stand still for 30min to obtain a soil suspension. Take 2mL of soil suspension and inoculate it into the enrichment medium, put it into a constant temperature incubator with a temperature of 25°C and a rotation speed of 220r / min for shaking culture for 3-5 days.

[0038] Draw 1mL of the above-mentioned enriched culture solution and inoculate it into the ferme...

Embodiment 2

[0047] The first step, the enrichment culture and domestication of strains

[0048] Specifically with embodiment 1.

[0049] The second step, the preparation of biosurfactant

[0050] After the acclimatized strains were continuously cultured in the enriched culture medium for 1 day, 1 mL of the bacterial liquid was inoculated into the fermentation medium at 4.5%, adjusted to a pH of 6, and cultured at a constant temperature at a temperature of 25°C and a rotation speed of 200r / min. In the tank, continue to shake and cultivate for 2.5 days, take out the fermentation broth, centrifuge, collect flocculent precipitates, and then use dichloromethane to resuspend, filter, extract, centrifuge, collect precipitates, and dry naturally at room temperature to obtain the purified biological surface active agent.

[0051] The third step, biosurfactant magnetically modified bentonite

[0052] Weigh 4g of bentonite into a 500mL three-neck flask, add 100mL of deionized water, 120mL of ethy...

Embodiment 3

[0057] The first step, the enrichment culture and domestication of strains

[0058] Specifically with embodiment 1.

[0059] The second step, the preparation of biosurfactant

[0060] After the acclimatized strains were continuously cultured in the enriched culture medium for 1 day, 1 mL of the bacterial liquid was inoculated into the fermentation medium at a rate of 4.5%, adjusted to a pH value of 7, and cultured at a constant temperature of 25°C and 200r / min. In the tank, continue to shake and cultivate for 2.5 days, take out the fermentation broth, centrifuge, collect flocculent precipitates, and then use dichloromethane to resuspend, filter, extract, centrifuge, collect precipitates, and dry naturally at room temperature to obtain the purified biological surface active agent.

[0061] The third step, biosurfactant modified magnetic bentonite

[0062]Weigh 5g of bentonite into a 500mL three-neck flask, add 100mL of deionized water, 120mL of ethylene glycol, and 60mL of p...

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Abstract

The invention relates to a method for modifying magnetic bentonite by a biosurfactant. The method comprises the steps of firstly burying straws and rice husks in farmland soil for composting, performing enrichment culture on compost and domesticating a biosurfactant to generate a bacterial strain, continuously performing fermental cultivation, re-suspending fermentation liquor by using dichloromethane, filtering, extracting, centrifuging, and collecting sediment, thus obtaining a purified biosurfactant. The purified biosurfactant is used for replacing a conventional chemical surfactant, modifying the magnetic bentonite, and preparing to obtain a novel adsorber; the novel adsorber is applied to removal of uranium in low-concentration uranium-containing wastewater. The adsorber has good adsorptive property, needs main raw materials of agricultural waste biomass and natural minerals, is pollution-free to environment, and truly realizes resource conservation and environment friendliness. The method is simple, mild in reaction condition, and easy in magnetism control, and the uranium content in the low-concentration uranium-containing wastewater with the concentration of 0.45-0.55 mg / L can be reduced by 95 percent or more.

Description

technical field [0001] The invention belongs to the field of resource utilization and environmental protection, and in particular relates to a method of biosurfactant-modified magnetic bentonite and its application in the treatment of uranium-containing wastewater. Background technique [0002] With the rapid development of my country's national economy and society, the development and utilization of new energy is becoming more and more important. Nuclear energy is a kind of clean energy, and its large-scale construction has been restarted one after another. A large number of nuclear power plants consume a lot of nuclear fuel while providing power for the national economy. As the main component of nuclear fuel, uranium will be mined and smelted on an increasing scale. Uranium is a radioactive element that has received widespread attention due to its long half-life and can be released into soil, sediment, and groundwater through mining operations, nuclear testing, nuclear fu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J20/12B01J20/28B01J20/30G21F9/12C12N1/36C12P1/00
CPCB01J20/12B01J20/28009C12N1/36C12P1/00G21F9/12
Inventor 肖方竹彭国文符建文谢睿余丽梅蒲移秋
Owner NANHUA UNIV
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